Hyaluronan size alters chondrogenesis of mesenchymal stem cells cultured on tricalcium phosphate‐collagen‐hyaluronan scaffolds

2021 ◽  
Author(s):  
Fangang Meng ◽  
Zibo Yang ◽  
Dianbo Long ◽  
Minghui Gu ◽  
Mei Shang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tricalcium Phosphate ◽  
Cells Cultured

Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid – Gelatin injectable hydrogels

2020 ◽  
Vol 134 ◽  
pp. 107536 ◽  
Author(s):  
Juan Jairo Vaca-González ◽  
Sandra Clara-Trujillo ◽  
María Guillot-Ferriols ◽  
Joaquín Ródenas-Rochina ◽  
María J. Sanchis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Hyaluronic Acid ◽  
Chondrogenic Differentiation ◽  
Injectable Hydrogels ◽  
Cells Cultured

In vitro Mineralization by Mesenchymal Stem Cells Cultured on Titanium Scaffolds

2007 ◽  
Vol 141 (5) ◽  
pp. 729-736 ◽  
Author(s):  
Masahiko Maeda ◽  
Motohiro Hirose ◽  
Hajime Ohgushi ◽  
Tadaaki Kirita
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Mineralization ◽  
Cells Cultured

Osteogenesis and angiogenesis of tissue-engineered bone constructed by prevascularized β-tricalcium phosphate scaffold and mesenchymal stem cells

Biomaterials ◽  
2010 ◽  
Vol 31 (36) ◽  
pp. 9452-9461 ◽  
Author(s):  
Le Wang ◽  
Hongbin Fan ◽  
Zhi-Yong Zhang ◽  
Ai-Ju Lou ◽  
Guo-Xian Pei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tricalcium Phosphate

scholarly journals Effects of Hypoxia and Chitosan on Equine Umbilical Cord-Derived Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
D. J. Griffon ◽  
J. Cho ◽  
J. R. Wagner ◽  
C. Charavaryamath ◽  
J. Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Negative Impact ◽  
Clinical Applications ◽  
Regenerative Therapy ◽  
Defect Model ◽  
Chitosan Films ◽  
Cells Cultured

Chitosan opens new perspectives in regenerative medicine as it enhances the properties of mesenchymal stem cells (MSCs) through formation of spheroids. Hypoxia has also been proposed to enhance stemness and survival of MSCs afterin vivoimplantation. These characteristics are relevant to the development of an off-the-shelf source of allogenic cells for regenerative therapy of tendinopathies. Umbilical cord-derived MSCs (UCM-MSCs) offer an abundant source of immature and immunoprivileged stem cells. In this study, equine UCM-MSCs (eqUCM-MSCs) conditioned for 3 and 7 days on chitosan films at 5% oxygen were compared to eqUCM-MSCs under standard conditions. Equine UCM-MSCs formed spheroids on chitosan but yielded 72% less DNA than standard eqUCM-MSCs. Expression ofSox2,Oct4, andNanogwas 4 to 10 times greater in conditioned cells at day 7. Fluorescence-labeled cells cultured for 7 days under standard conditions or on chitosan films under hypoxia were compared in a bilateral patellar tendon defect model in rats. Fluorescence was present in all treated tendons, but the modulus of elasticity under tension was greater in tendons treated with conditioned cells. Chitosan and hypoxia affected cell yield but improved the stemness of eqUCM-MSCs and their contribution to the healing of tissues. Given the abundance of allogenic cells, these properties are highly relevant to clinical applications and outweigh the negative impact on cell proliferation.

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