Visible light–induced cross‐linking of porcine pericardium for the improvement of endothelialization, anti‐tearing, and anticalcification properties

Author(s):  
Fan Yang ◽  
Liangpeng Xu ◽  
Gaoyang Guo ◽  
Yunbing Wang
2020 ◽  
Vol 30 (26) ◽  
pp. 2070173
Author(s):  
Jordan P. Hooker ◽  
Florian Feist ◽  
Laura Delafresnaye ◽  
Leonie Barner ◽  
Christopher Barner‐Kowollik

2019 ◽  
Vol 19 (6) ◽  
pp. 1900098 ◽  
Author(s):  
Khoon S. Lim ◽  
Barbara J. Klotz ◽  
Gabriella C. J. Lindberg ◽  
Ferry P. W. Melchels ◽  
Gary J. Hooper ◽  
...  

2020 ◽  
Vol 2 (11) ◽  
pp. 4802-4808
Author(s):  
Taylor Wright ◽  
Tanja Tomkovic ◽  
Savvas G. Hatzikiriakos ◽  
Michael O. Wolf

2019 ◽  
Vol 30 (26) ◽  
pp. 1905399 ◽  
Author(s):  
Jordan P. Hooker ◽  
Florian Feist ◽  
Laura Delafresnaye ◽  
Leonie Barner ◽  
Christopher Barner‐Kowollik
Keyword(s):  

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Jin Wook Hwang ◽  
Sungtae Kim ◽  
Se Won Kim ◽  
Jong Ho Lee

Absorbable extracellular matrix (ECM) membrane has recently been used as a barrier membrane (BM) in guided tissue regeneration (GTR) and guided bone regeneration (GBR). Absorbable BMs are mostly based on collagen, which is more biocompatible than synthetic materials. However, implanted absorbable BMs can be rapidly degraded by enzymesin vivo. In a previous study, to delay degradation time, collagen fibers were treated with cross-linking agents. These compounds prevented the enzymatic degradation of BMs. However, cross-linked BMs can exhibit delayed tissue integration. In addition, the remaining cross-linker could induce inflammation. Here, we attempted to overcome these problems using a natural ECM membrane. The membrane consisted of freshly harvested porcine pericardium that was stripped from cells and immunoreagents by a cleaning process. Acellular porcine pericardium (APP) showed a bilayer structure with a smooth upper surface and a significantly coarser bottom layer. APP is an ECM with a thin layer (0.18–0.35 mm) but with excellent mechanical properties. Tensile strength of APP was14.15±2.24 MPa. Inin vivoexperiments, APP was transplanted into rabbit tibia. The biocompatible material was retained for up to 3 months without the need for cross-linking. Therefore, we conclude that APP could support osteogenesis as a BM for up to 3 months.


Polyvinyl bromide in solution, in dry tetrahydrofuran (THF) yields a black polymer upon exposure to u.v. light of 260 nm or less. This is shown to be the result of ( a ) the elimination of bromine atoms in the primary reaction, and ( b ) the catalysed formation of a conjugated chain by the eliminated bromine atoms. The black polymer is bleached by visible light with the formation of a cross-linked product. This blackening takes place in the full light of the mercury lamp, but by the introduction of a suitable filter passing only visible light, the colour changes can be arrested and ultimately reversed though the viscosity continues to increase due to cross-linking. In dry solutions the liberated bromine is absorbed by the THF solvent and no free Br 2 or HBr is produced. In the presence of water, the products are completely changed. No blackening occurs, free HBr is eliminated, and the colourless PVB becomes cross-linked and ultimately yields a gel. On irradiating the black polymer in aqueous THF by visible light, it becomes bleached, yielding a cross-linked colourless polymer which ultimately gels.


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