scholarly journals Black phosphorus incorporation modulates nanocomposite hydrogel properties and subsequent MC3T3 cell attachment, proliferation, and differentiation

2021 ◽  
Author(s):  
Haocheng Xu ◽  
Xifeng Liu ◽  
Matthew N. George ◽  
A. Lee Miller ◽  
Sungjo Park ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Black Phosphorus ◽  
Nanocomposite Hydrogel ◽  
Mc3t3 Cell ◽  
Proliferation And Differentiation

Glycans in scaffold design in tissue reconstruction

2021 ◽  
pp. 088391152199784
Author(s):  
Nipun Jain ◽  
Shashi Singh
Keyword(s):  
Cell Attachment ◽  
Low Cost ◽  
Growth Conditions ◽  
Downstream Signaling ◽  
Cell Carrier ◽  
Wide Range ◽  
Proliferation And Differentiation ◽  
Different Types ◽  
Tissue Reconstruction ◽  
A Cell

Development of an artificial tissue by tissue engineering is witnessed to be one of the long lasting clarified solutions for the damaged tissue function restoration. To accomplish this, a scaffold is designed as a cell carrier in which the extracellular matrix (ECM) performs a prominent task of controlling the inoculated cell’s destiny. ECM composition, topography and mechanical properties lead to different types of interactions between cells and ECM components that trigger an assortment of cellular reactions via diverse sensing mechanisms and downstream signaling pathways. The polysaccharides in the form of proteoglycans and glycoproteins yield better outcomes when included in the designed matrices. Glycosaminoglycan (GAG) chains present on proteoglycans show a wide range of operations such as sequestering of critical effector morphogens which encourage proficient nutrient contribution toward the growing stem cells for their development and endurance. In this review we discuss how the glycosylation aspects are of considerable importance in everyday housekeeping functions of a cell especially when placed in a controlled environment under ideal growth conditions. Hydrogels made from these GAG chains have been used extensively as a resorbable material that mimics the natural ECM functions for an efficient control over cell attachment, permeability, viability, proliferation, and differentiation processes. Also the incorporation of non-mammalian polysaccharides can elicit specific receptor responses which authorize the creation of numerous vigorous frameworks while prolonging the low cost and immunogenicity of the substance.

scholarly journals Drug-Loaded Biomimetic Ceramics for Tissue Engineering

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 272 ◽  
Author(s):  
Patricia Diaz-Rodriguez ◽  
Mirian Sánchez ◽  
Mariana Landin
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Release Kinetics ◽  
Drug Loading ◽  
Critical Parameters ◽  
Tissue Engineering Scaffolds ◽  
Biomimetic Scaffolds ◽  
Proliferation And Differentiation ◽  
Therapeutic Molecules ◽  
Biological Performance

The mimesis of biological systems has been demonstrated to be an adequate approach to obtain tissue engineering scaffolds able to promote cell attachment, proliferation, and differentiation abilities similar to those of autologous tissues. Bioceramics are commonly used for this purpose due to their similarities to the mineral component of hard tissues as bone. Furthermore, biomimetic scaffolds are frequently loaded with diverse therapeutic molecules to enhance their biological performance, leading to final products with advanced functionalities. In this review, we aim to describe the already developed bioceramic-based biomimetic systems for drug loading and local controlled release. We will discuss the mechanisms used for the inclusion of therapeutic molecules on the designed systems, paying special attention to the identification of critical parameters that modulate drug loading and release kinetics on these scaffolds.

Proliferation and Differentiation of MC3T3-E1 Cells on Calcium Phosphate/Chitosan Coatings

2008 ◽  
Vol 87 (7) ◽  
pp. 650-654 ◽  
Author(s):  
J. Wang ◽  
J. de Boer ◽  
K. de Groot
Keyword(s):  
Calcium Phosphate ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Bone Sialoprotein ◽  
Differentiation Potential ◽  
Collagen Expression ◽  
Significant Difference ◽  
Proliferation And Differentiation ◽  
Proliferative Ability

The incorporation of chitosan into electro-deposited calcium phosphate (CaP) coatings increases bone marrow stromal cell attachment. We hypothesized that such electrodeposited CaP/chitosan coatings can also enhance the proliferative ability and differentiation potential of osteoblasts. To verify this hypothesis, we cultured osteoblast-like MC3T3-E1 cells on these CaP coatings. It was found that MC3T3-E1 cells cultured on the electrodeposited CaP/chitosan coatings had cell proliferation rates higher than those on the electrodeposited CaP coatings. At the same time, both alkaline phosphatase activity and collagen expression were increased, and both bone sialoprotein and osteocalcin genes were up-regulated when MC3T3-E1 cells were cultured on the electrodeposited CaP/chitosan coatings. Additionally, within the range of selected chitosan concentrations in solution, no significant difference was found between the CaP/chitosan coatings. Our results suggest that the electrodeposited CaP/chitosan coatings are favorable to the proliferation and differentiation of MC3T3-E1 cells, which may endow them with great potential for future applications.

Nanoscale RGD Peptide Organization Regulates Cell Proliferation and Differentiation

2004 ◽  
Vol 845 ◽  
Author(s):  
Susan Hsiong ◽  
Kuen Yong Lee ◽  
Eben Alsberg ◽  
David Mooney
Keyword(s):  
Cell Attachment ◽  
Cell Types ◽  
Important Variable ◽  
Rgd Peptide ◽  
Polymer Chains ◽  
Cell Phenotype ◽  
Rgd Peptides ◽  
Ligand Density ◽  
Proliferation And Differentiation

ABSTRACTRGD (arginine-glycine-aspartic acid) containing peptide sequences, common cell attachment sites present in many extracellular matrix (ECM) proteins, mediate many important cellular processes. The role of nanoscale organization of RGD peptides in the regulation of the adhesion, proliferation and differentiation of both preosteoblasts (MC3T3-E1) and multipotential (D1) cell lines in vitro was investigated in this study. Alginate polymer chains with varying RGD peptide degree of substitution were mixed with unmodified polymer chains at different ratios to allow variation of RGD peptide spacing in the nanometer scale, independently of the overall bulk density of peptides presented from the material. Proliferation of both cell types was observed to be closely correlated to RGD island (defined as a cluster of RGD peptides) spacing, independently of overall bulk ligand density, following cell adhesion to alginate hydrogels. Increased RGD island spacing was observed to promote spreading of MC3T3-E1 cells while simultaneously suppressing their proliferation. However, increased RGD island spacing decreased spreading of D1 cells while also decreasing proliferation. Moreover, differentiation of preosteoblasts was significantly upregulated in response to decreased RGD island spacing, whereas differentiation of multipotential cells was modestly regulated by this variable. These results demonstrate that the nanoscale organization of adhesion ligands may be an important variable in controlling cell phenotype and function. In addition, cellular responses to nanoscale ligand organization differ depending on the cell type, and this may be related to the differentiation stage of the cells.

scholarly journals Flexible, Biocompatible PET Sheets: A Platform for Attachment, Proliferation and Differentiation of Eukaryotic Cells

Surfaces ◽  
2021 ◽  
Vol 4 (4) ◽  
pp. 306-322
Author(s):  
Soumen Samanta ◽  
Diana Gaad ◽  
Eva Cabet ◽  
Alain Lilienbaum ◽  
Ajay Singh ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Muscle Fibers ◽  
Eukaryotic Cells ◽  
Glass Slides ◽  
Free Radical Photopolymerization ◽  
Proliferation And Differentiation ◽  
Coupling Procedure ◽  
Positive Effect ◽  
Oriented Polyethylene

Transparent, flexible, biaxially oriented polyethylene terephthalate (PET) sheets were modified by bioactive polymer-fibronectin top layers for the attachment of cells and growth of muscle fibers. Towards this end, PET sheets were grafted with 4-(dimethylamino)phenyl (DMA) groups from the in situ generated diazonium cation precursor. The arylated sheets served as macro-hydrogen donors for benzophenone and the growth of poly(2-hydroxy ethyl methacrylate) (PHEMA) top layer by surface-confined free radical photopolymerization. The PET-PHEMA sheets were further grafted with fibronectin (FBN) through the 1,1-carbonyldiimidazole coupling procedure. The bioactive PET-PHEMA-I-FBN was then employed as a platform for the attachment, proliferation and differentiation of eukaryotic cells which after a few days gave remarkable muscle fibers, of ~120 µm length and ~45 µm thickness. We demonstrate that PET-PHEMA yields a fast growth of cells followed by muscle fibers of excellent levels of differentiation compared to pristine PET or standard microscope glass slides. The positive effect is exacerbated by crosslinking PHEMA chains with ethylene glycol dimethacrylate at initial HEMA/EGDA concentration ratio = 9/1. This works conclusively shows that in situ generated diazonium salts provide aryl layers for the efficient UV-induced grafting of biocompatible coating that beneficially serve as platform for cell attachment and growth of muscle fibers.

scholarly journals Graphene: A Versatile Carbon-Based Material for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Nileshkumar Dubey ◽  
Ricardo Bentini ◽  
Intekhab Islam ◽  
Tong Cao ◽  
Antonio Helio Castro Neto ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Attachment ◽  
High Surface ◽  
High Surface Area ◽  
Reduced Graphene ◽  
Proliferation And Differentiation ◽  
Cell Growth And Differentiation ◽  
Large Bone ◽  
Carbon Based

The development of materials and strategies that can influence stem cell attachment, proliferation, and differentiation towards osteoblasts is of high interest to promote faster healing and reconstructions of large bone defects. Graphene and its derivatives (graphene oxide and reduced graphene oxide) have received increasing attention for biomedical applications as they present remarkable properties such as high surface area, high mechanical strength, and ease of functionalization. These biocompatible carbon-based materials can induce and sustain stem cell growth and differentiation into various lineages. Furthermore, graphene has the ability to promote and enhance osteogenic differentiation making it an interesting material for bone regeneration research. This paper will review the important advances in the ability of graphene and its related forms to induce stem cells differentiation into osteogenic lineages.

scholarly journals Development of magnesium calcium phosphate biocement for bone regeneration

2010 ◽  
Vol 7 (49) ◽  
pp. 1171-1180 ◽  
Author(s):  
Junfeng Jia ◽  
Huanjun Zhou ◽  
Jie Wei ◽  
Xin Jiang ◽  
Hong Hua ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Cell Attachment ◽  
Setting Time ◽  
Molar Ratio ◽  
Dihydrogen Phosphate ◽  
Good Ability ◽  
Degradation Ratio ◽  
Close Proximity ◽  
Proliferation And Differentiation ◽  
Fast Dissolution

Magnesium calcium phosphate biocement (MCPB) with rapid-setting characteristics was fabricated by using the mixed powders of magnesium oxide (MgO) and calcium dihydrogen phosphate (Ca(H 2 PO 4 ) 2 ·H 2 O). The results revealed that the MCPB hardened after mixing the powders with water for about 7 min, and the compressive strength reached 43 MPa after setting for 1 h, indicating that the MCPB had a short setting time and high initial mechanical strength. After the acid–base reaction of MCPB containing MgO and Ca(H 2 PO 4 ) 2 ·H 2 O in a molar ratio of 2 : 1, the final hydrated products were Mg 3 (PO 4 ) 2 and Ca 3 (PO 4 ) 2 . The MCPB was degradable in Tris–HCl solution and the degradation ratio was obviously higher than calcium phosphate biocement (CPB) because of its fast dissolution. The attachment and proliferation of the MG 63 cells on the MCPB were significantly enhanced in comparison with CPB, and the alkaline phosphatase activity of MG 63 cells on the MCPB was significantly higher than on the CPB at 7 and 14 days. The MG 63 cells with normal phenotype spread well on the MCPB surfaces, and were attached in close proximity to the substrate, as seen by scanning electron microscopy (SEM). The results demonstrated that the MCPB had a good ability to support cell attachment, proliferation and differentiation, and exhibited good cytocompatibility.

scholarly journals Cellular Performance Comparison of Biomimetic Calcium Phosphate Coating and Alkaline-Treated Titanium Surface

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaohua Yu ◽  
Mei Wei
Keyword(s):  
Calcium Phosphate ◽  
Titanium Surface ◽  
Cell Attachment ◽  
Substrate Surface ◽  
Calcium Phosphate Coating ◽  
Osteoblastic Cell ◽  
Phosphate Coating ◽  
Proliferation And Differentiation ◽  
The Impact

The influence of biomimetic calcium phosphate coating on osteoblasts behaviorin vitrois not well established yet. In this study, we investigated the behavior of osteoblastic rat osteosarcoma 17/2.8 cells (ROS17/2.8) on two groups of biomaterial surfaces: alkaline-treated titanium surface (ATT) and biomimetic calcium phosphate coated ATT (CaP). The cell attachment, proliferation, differentiation, and morphology on these surfaces were extensively evaluated to reveal the impact of substrate surface on osteoblastic cell responses. It was found that the ROS17/2.8 cells cultured on the ATT surface had higher attachment and proliferation rates compared to those on the CaP surface. Our results also showed that the calcium phosphate coatings generated in this work have an inhibiting effect on osteoblast adhesion and further influenced the proliferation and differentiation of osteoblast compared to the ATT surfacein vitro. Cells on the ATT surface also exhibited a higher alkaline phosphatase activity than on the CaP surface after two weeks of culture. Immunofluorescence staining and scanning electron microscopy results showed that the cells adhered and spread faster on the ATT surface than on the CaP surface. These results collectively suggested that substrate surface properties directly influence cell adhesion on different biomaterials, which would result in further influence on the cell proliferation and differentiation.

scholarly journals Mechanisms of Endothelial Cell Attachment, Proliferation, and Differentiation on 4 Types of Platinum-Based Endovascular Coils

2014 ◽  
Vol 82 (5) ◽  
pp. 684-695 ◽  
Author(s):  
Aditya S. Pandey ◽  
James D. San Antonio ◽  
Sankar Addya ◽  
Saul Surrey ◽  
Paolo Fortina ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Attachment ◽  
Proliferation And Differentiation ◽  
Endothelial Cell Attachment ◽  
Endovascular Coils
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