Platelet microparticles load a repertory of miRNAs programmed to drive osteogenic phenotype

2020 ◽  
Author(s):  
Marcel Rodrigues Ferreira ◽  
Willian Fernando Zambuzzi
Keyword(s):  
Platelet Microparticles

scholarly journals Calpain activity in patients with thrombotic thrombocytopenic purpura is associated with platelet microparticles

Blood ◽  
1992 ◽  
Vol 80 (9) ◽  
pp. 2246-2251 ◽  
Author(s):  
JG Kelton ◽  
TE Warkentin ◽  
CP Hayward ◽  
WG Murphy ◽  
JC Moore
Keyword(s):  
Thrombotic Thrombocytopenic Purpura ◽  
Membrane Association ◽  
Membrane Glycoproteins ◽  
Calpain Activity ◽  
Thrombocytopenic Purpura ◽  
Calcium Dependent ◽  
Active Protease ◽  
Triton X ◽  
Platelet Microparticles

Abstract Thrombotic thrombocytopenic purpura (TTP) is characterized by thrombocytopenia and disseminated platelet thrombi throughout the microvasculature. Studies by our group have demonstrated calcium- dependent proteolytic activity (calpain) that is no longer detectable in the serum of patients with acute TTP after their recovery. The purpose of this study was to investigate if the protease activity of TTP was detectable in plasma and, therefore, not an in vitro phenomenon secondary to the formation of serum. Additionally, we looked for evidence of membrane association of the active protease in the patients' samples, which would explain the persistence of its activity in the presence of plasma inhibitors. Acute TTP samples, both serum and plasma, were collected from 10 patients with TTP. Calpain was measured using bioassays for enzyme activity and also by detection of the protein using immunoblotting with an anticalpain monoclonal antibody (MoAb). In all instances, calpain could be detected both functionally and antigenically in the acute TTP sera and plasma. No calpain activity could be detected in any of the controls, although antigenic calpain was detectable in one sample from a patient who had undergone cardiopulmonary bypass surgery. To investigate whether the calpain was associated with microparticles in the plasma, the TTP plasma samples were ultrafiltered and ultracentrifuged. Activity was not lost by passage across a 0.2-micron filter but was detectable only in the pellet following ultracentrifugation. Membrane association of the calpain in the microparticles also was demonstrated using solubilization with Triton X-100. Immunoprecipitation studies demonstrated that the calpain activity could be removed by MoAbs against platelet membrane glycoproteins (IX and IIb/IIa) but not by a MoAb against red blood cell membrane glycophorin. These studies indicate that active calpain is associated with platelet microparticles in plasma from patients with TTP.

Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticles

2020 ◽  
Vol 18 (8) ◽  
pp. 620-627
Author(s):  
Fang-Fang CHEN ◽  
Meng-Meng WANG ◽  
Wen-Wen XIA ◽  
Dai-Yin PENG ◽  
Lan HAN
Keyword(s):  
Cerebral Ischaemia ◽  
Platelet Microparticles

Decrease in circulating percentage platelet microparticles during pregnancy—a different perspective

2015 ◽  
Vol 95 (3) ◽  
pp. 533-534
Author(s):  
Rucha Patil ◽  
Kanjaksha Ghosh ◽  
Shrimati Shetty
Keyword(s):  
Platelet Microparticles

Platelet microparticles are released from blood platelets and bone marrow cells and stimulate endothelial invasion and neovascularisation

2006 ◽  
Vol 45 (3) ◽  
pp. e120-e121
Author(s):  
Lukasz Partyka ◽  
Joanna Grzybowska ◽  
Urszula Czech ◽  
Anna Polus ◽  
Lukasz Wator ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Blood Platelets ◽  
Platelet Microparticles ◽  
Marrow Cells

scholarly journals Platelet microparticles induce angiogenesis in vitro

2004 ◽  
Vol 124 (3) ◽  
pp. 376-384 ◽  
Author(s):  
Hyun Kyung Kim ◽  
Kyung Soon Song ◽  
Jun-Ho Chung ◽  
Kyoung Rhan Lee ◽  
Se-Na Lee
Keyword(s):  
Platelet Microparticles

scholarly journals Plasma centrifugation does not influence thrombin-antithrombin and plasmin-antiplasmin levels but determines platelet microparticles count

2015 ◽  
Vol 25 (2) ◽  
pp. 222-229 ◽  
Author(s):  
Ewa Stępień ◽  
Krzysztof Gruszczyński ◽  
Przemysław Kapusta ◽  
Artur Kowalik ◽  
Iwona Wybrańska
Keyword(s):  
Platelet Microparticles
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