Control of cellular adhesiveness in hyaluronic acid‐based hydrogel through varying degrees of phenol moiety cross‐linking

2020 ◽  
Author(s):  
Sara Bagheri ◽  
Zohreh Bagher ◽  
Sajad Hassanzadeh ◽  
Sara Simorgh ◽  
Seyed Kamran Kamrava ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cross Linking ◽  
Phenol Moiety

scholarly journals Toward Physicochemical and Rheological Characterization of Different Injectable Hyaluronic Acid Dermal Fillers Cross-Linked with Polyethylene Glycol Diglycidyl Ether

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 948
Author(s):  
Nicola Zerbinati ◽  
Sabrina Sommatis ◽  
Cristina Maccario ◽  
Maria Chiara Capillo ◽  
Giulia Grimaldi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Polyethylene Glycol ◽  
Biological Activities ◽  
Diglycidyl Ether ◽  
Dermal Filler ◽  
Cross Linking ◽  
Matrix Structure ◽  
Dermal Fillers ◽  
Rheological Characterization

(1) Background: Injectable hyaluronic acid (HA) dermal fillers are used to restore volume, hydration and skin tone in aesthetic medicine. HA fillers differ from each other due to their cross-linking technologies, with the aim to increase mechanical and biological activities. One of the most recent and promising cross-linkers is polyethylene glycol diglycidyl ether (PEGDE), used by the company Matex Lab S.p.A., (Brindisi, Italy) to create the HA dermal filler PEGDE family. Over the last few years, several studies have been performed to investigate the biocompatibility and biodegradability of these formulations, but little information is available regarding their matrix structure, rheological and physicochemical properties related to their cross-linking technologies, the HA content or the degree of cross-linking. (2) Methods: Seven different injectable HA hydrogels were subjected to optical microscopic examination, cohesivity evaluation and rheological characterization in order to investigate their behavior. (3) Results: The analyzed cross-linked dermal fillers showed a fibrous “spiderweb-like” matrix structure, with each medical device presenting different and peculiar rheological features. Except for HA non cross-linked hydrogel 18 mg/mL, all showed an elastic and cohesive profile. (4) Conclusions: The comparative analysis with other literature works makes a preliminary characterization of these injectable medical devices possible.

scholarly journals 3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches

2019 ◽  
Vol 6 (2) ◽  
pp. 30 ◽  
Author(s):  
Chiara Campiglio ◽  
Francesca Ceriani ◽  
Lorenza Draghi
Keyword(s):  
Hyaluronic Acid ◽  
Regenerative Medicine ◽  
Closed Loop ◽  
Fiber Diameter ◽  
Cross Linking ◽  
Scaffold Fabrication ◽  
Cell Culturing ◽  
Viable Cells ◽  
Flow Circuit

To fully exploit the potential of hydrogel micro-fibers in the design of regenerative medicinal materials, we designed a simple, easy to replicate system for cell embedding in degradable fibrous scaffolds, and validated its effectiveness using alginate-based materials. For scaffold fabrication, cells are suspended in a hydrogel-precursor and injected in a closed-loop circuit, where a pump circulates the ionic cross-linking solution. The flow of the cross-linking solution stretches and solidifies a continuous micro-scaled, cell-loaded hydrogel fiber that whips, bends, and spontaneously assembles in a self-standing, spaghetti-like patch. After investigation and tuning of process- and solution-related parameters, homogeneous microfibers with controlled diameters and consistent scaffolds were obtained from different alginate concentrations and blends with biologically favorable macromolecules (i.e., gelatin or hyaluronic acid). Despite its simplicity, this coaxial-flow encapsulation system allows for the rapid and effortless fabrication of thick, well-defined scaffolds, with viable cells being homogeneously distributed within the fibers. The reduced fiber diameter and the inherent macro-porous structure that is created from the random winding of fibers can sustain mass transport, and support encapsulated cell survival. As different materials and formulations can be processed to easily create homogeneously cell-populated structures, this system appears as a valuable platform, not only for regenerative medicine, but also, more in general, for 3D cell culturing in vitro.

Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking

Biomaterials ◽  
2002 ◽  
Vol 23 (4) ◽  
pp. 1205-1212 ◽  
Author(s):  
Si-Nae Park ◽  
Jong-Chul Park ◽  
Hea Ok Kim ◽  
Min Jung Song ◽  
Hwal Suh
Keyword(s):  
Hyaluronic Acid ◽  
Cross Linking

The effect of oxidation degree and volume ratio of components on properties and applications of in situ cross-linking hydrogels based on chitosan and hyaluronic acid

2019 ◽  
Vol 103 ◽  
pp. 109670 ◽  
Author(s):  
Nghi Thi-Phuong Nguyen ◽  
Long Vuong-Hoang Nguyen ◽  
Nam Minh-Phuong Tran ◽  
Dat Tan Nguyen ◽  
Tien Ngoc-Thuy Nguyen ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Volume Ratio ◽  
Cross Linking ◽  
Oxidation Degree ◽  
Ratio Of Components

Effect of Chemical Cross-linking on Properties of Gelatin/Hyaluronic Acid Composite Hydrogels

2013 ◽  
Vol 52 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Zhihua Zhou ◽  
Zhongmin Yang ◽  
Tianlong Huang ◽  
Lihua Liu ◽  
Qingquan Liu ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cross Linking ◽  
Composite Hydrogels ◽  
Chemical Cross Linking

Hyaluronic acid and alginate covalent nanogels by template cross-linking in polyion complex micelle nanoreactors

2014 ◽  
Vol 101 ◽  
pp. 96-103 ◽  
Author(s):  
Serena De Santis ◽  
Marco Diociaiuti ◽  
Cesare Cametti ◽  
Giancarlo Masci
Keyword(s):  
Hyaluronic Acid ◽  
Cross Linking ◽  
Polyion Complex

A novel DTPA cross-linking of hyaluronic acid and metal complexation thereof

2011 ◽  
Vol 346 (13) ◽  
pp. 1909-1915 ◽  
Author(s):  
Radovan Buffa ◽  
Jiří Běťák ◽  
Sofiane Kettou ◽  
Martina Hermannová ◽  
Lucie Pospíšilová ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Metal Complexation ◽  
Cross Linking

scholarly journals Clinicians’ Perspectives on the Use of Intra-Articular Hyaluronic Acid as a Treatment for Knee Osteoarthritis: A North American, Multidisciplinary Survey

2016 ◽  
Vol 9 ◽  
pp. CMAMD.S34496 ◽  
Author(s):  
Jeffrey Rosen ◽  
Victoria Avram ◽  
Anke Fierlinger ◽  
Faizan Niazi ◽  
Parag Sancheti ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Knee Osteoarthritis ◽  
Patient Outcomes ◽  
Current Literature ◽  
Early Stage ◽  
Sodium Hyaluronate ◽  
Cross Linking ◽  
Product Characteristics ◽  
Knee Oa ◽  
Orthopedic Surgeons

Introduction This study aims to describe the perceptions of orthopedic surgeons on the efficacy of intra-articular hyaluronic acid (IA-HA), the influence of IA-HA product characteristics on its efficacy, and to identify patterns and factors related to the use of IA-HA. Additionally, this study examines factors that influence IA-HA brand selection, focusing on Euflexxa¯ (1% sodium hyaluronate). Methods We developed survey questions by reviewing the current literature and consulting with experts on the use of IA-HA in the management of knee osteoarthritis (OA). The survey included questions on demographics, previous experience with knee OA treatment, opinions on different treatment methods, and where information regarding treatments is obtained. Additionally, questions specific to opinions regarding IA-HA and the reasoning behind these opinions were asked. Results A total of 117 orthopedic surgeons and physicians completed the survey. IA-HA is most frequently prescribed to patients with early-stage (82%) or mid-stage (82.8%) OA, while fewer orthopedic surgeons and physicians use IA-HA for patients with late-stage OA (57.4%). Respondents were generally uncertain of the effects that intrinsic characteristics, such as molecular weight, cross-linking, and production process, had on patient outcomes. Respondents typically use their own clinical experience and results as a deciding factor in utilizing IA-HA treatment, as well as in choosing an IA-HA brand. Conclusion Uncertainty regarding the efficacy of IA-HA treatments is likely due to inconsistency within clinical guidelines and the current literature. Additional research investigating the efficacy of IA-HA treatment and how product characteristics affect outcome and safety is required to provide clarity to the controversy surrounding IA-HA treatment for knee OA.

scholarly journals Hyaluronic Acid Based Hydrogels for Regenerative Medicine Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Assunta Borzacchiello ◽  
Luisa Russo ◽  
Birgitte M. Malle ◽  
Khadija Schwach-Abdellaoui ◽  
Luigi Ambrosio
Keyword(s):  
Hyaluronic Acid ◽  
Viscoelastic Properties ◽  
Structural Parameters ◽  
Weight Ratio ◽  
Rheological Behaviour ◽  
Biological Properties ◽  
Cross Linking ◽  
In Vitro Tests ◽  
The Cross

Hyaluronic acid (HA) hydrogels, obtained by cross-linking HA molecules with divinyl sulfone (DVS) based on a simple, reproducible, and safe process that does not employ any organic solvents, were developed. Owing to an innovative preparation method the resulting homogeneous hydrogels do not contain any detectable residual cross-linking agent and are easier to inject through a fine needle. HA hydrogels were characterized in terms of degradation and biological properties, viscoelasticity, injectability, and network structural parameters. They exhibit a rheological behaviour typical of strong gels and show improved viscoelastic properties by increasing HA concentration and decreasing HA/DVS weight ratio. Furthermore, it was demonstrated that processes such as sterilization and extrusion through clinical needles do not imply significant alteration of viscoelastic properties. Both SANS and rheological tests indicated that the cross-links appear to compact the network, resulting in a reduction of the mesh size by increasing the cross-linker amount. In vitro degradation tests of the HA hydrogels demonstrated that these new hydrogels show a good stability against enzymatic degradation, which increases by increasing HA concentration and decreasing HA/DVS weight ratio. Finally, the hydrogels show a good biocompatibility confirmed by in vitro tests.

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