The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells

2018 ◽  
Vol 107 (1) ◽  
pp. 174-186 ◽  
Author(s):  
Milda Alksne ◽  
Egidijus Simoliunas ◽  
Migle Kalvaityte ◽  
Edvinas Skliutas ◽  
Ieva Rinkunaite ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Polylactic Acid ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Cell Diameter ◽  
Surface Patterns

scholarly journals Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells

2021 ◽  
Vol 6 (9) ◽  
pp. 2742-2751
Author(s):  
Myung Chul Lee ◽  
Hoon Seonwoo ◽  
Kyoung Je Jang ◽  
Shambhavi Pandey ◽  
Jaewoon Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Gene Carrier ◽  
Novel Gene ◽  
Equine Bone

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals A Comparative In Vitro Analysis of the Osteogenic Potential of Human Dental Pulp Stem Cells Using Various Differentiation Conditions

2020 ◽  
Vol 21 (7) ◽  
pp. 2280 ◽  
Author(s):  
Terezia Okajcekova ◽  
Jan Strnadel ◽  
Michal Pokusa ◽  
Romana Zahumenska ◽  
Maria Janickova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Osteogenic Potential ◽  
Surface Markers ◽  
Osteogenic Medium ◽  
Differentiated Cells ◽  
Mineralization Potential ◽  
Gene And Protein Expression

Dental pulp stem cells (DPSCs) have excellent proliferative properties, mineralization potential and can be easily obtained from third molar teeth. Recently, many studies have focused on isolation and differentiation of DPSCs. In our study, we focused on biological properties of non-differentiated DPSCs in comparison with osteogenic differentiated cells from DPSCs. We analyzed morphology as well as mineralization potential using three varied osteogenic differentiation media. After fifteen days of differentiation, calcium deposit production was observed in all three osteogenic differentiation media. However, only one osteogenic medium, without animal serum supplement, showed rapid and strong calcification—OsteoMAX-XF™ Differentiation Medium. Therefore, we examined specific surface markers, and gene and protein expression of cells differentiated in this osteogenic medium, and compared them to non-differentiated DPSCs. We proved a decrease in expression of CD9 and CD90 mesenchymal stem cell surface markers, as well as downregulation in the expression of pluripotency genes (NANOG and OCT-4) and increased levels of expression in osteogenic genes (ALP, BSP, OCN and RUNX2). Moreover, osteogenic proteins, such as BSP and OCN, were only produced in differentiated cells. Our findings confirm that carefully selected differentiation conditions for stem cells are essential for their translation into future clinical applications.

Effects of resveratrol on the proliferation and osteogenic differentiation of deciduous dental pulp stem cells from neurofibromatosis type 1 patient

2020 ◽  
Author(s):  
Claudinéia Pereira Maranduba ◽  
Gustavo Torres Souza ◽  
Antônio Márcio Resende do Carmo ◽  
José Marcelo Sallabert de Campos ◽  
Nádia Rezende Barbosa Raposo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Neurofibromatosis Type 1 ◽  
Dental Pulp ◽  
Neurofibromatosis Type ◽  
Dental Pulp Stem Cells
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