scholarly journals Fibrinogen adsorption to biomaterials

2018 ◽  
Vol 106 (10) ◽  
pp. 2777-2788 ◽  
Author(s):  
Thomas A. Horbett
Keyword(s):  
Fibrinogen Adsorption

Baboon Fibrinogen Adsorption and Platelet Adhesion to Polymeric Materials

1991 ◽  
Vol 65 (05) ◽  
pp. 608-617 ◽  
Author(s):  
Joseph A Chinn ◽  
Thomas A Horbett ◽  
Buddy D Ratner
Keyword(s):  
Platelet Adhesion ◽  
Coagulation Factor ◽  
Polymeric Materials ◽  
Platelet Suspension ◽  
Perfusion Chamber ◽  
Static Conditions ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Fibrinogen Adsorption

SummaryThe role of fibrinogen in mediating platelet adhesion to polymers exposed to blood plasma was studied by comparison of the effect of plasma dilution on fibrinogen adsorption and platelet adhesion, and by the use of coagulation factor deficient plasmas. Polyetherurethane substrates were first preadsorbed with dilute plasma, then contacted with washed platelets suspended in a modified, apyrase containing Tyrode’s buffer. Platelet adhesion was studied under static conditions in Multiwell dishes, and also under shearing conditions using a parallel plate perfusion chamber. Fibrinogen adsorption and platelet adhesion were measured using 125I radiolabeled baboon fibrinogen and min radiolabeled baboon platelets, respectively. Surfaces were characterized by electron spectroscopy for chemical analysis (ESCA).When fibrinogen adsorption to Biomer was measured after 2 h contact with a series of dilute plasma solutions under static conditions, a peak in adsorption was observed from 0.26% plasma, i.e., adsorption was greater from 0.26% plasma than from either more or less dilute plasma. A peak in subsequent platelet adhesion to the plasma preadsorbed surfaces, measured after 2 h static incubation with washed platelets, was also observed but occurred on Biomer preadsorbed with 1.0% plasma.When fibrinogen adsorption was measured after 5 min contact under shearing conditions, the fibrinogen adsorption peak occurred on surfaces that had been exposed to 1.0% plasma. A peak in platelet adhesion to these preadsorbed surfaces, measured after 5 min contact with the platelet suspensions under shearing conditions, was observed on Biomer preadsorbed with 0.1% plasma. Shifts between the positions of the peaks in protein adsorption and platelet adhesion occurred on other polymers tested as well.Platelet adhesion was almost completely inhibited when baboon and human plasmas lacking fibrinogen (i. e., serum, heat defibrinogenated plasma, and congenitally afibrinogénémie plasma) were used. Platelet adhesion was restored to near normal when exogenous fibrinogen was added to fibrinogen deficient plasmas. Adhesion was also inhibited completely when a monoclonal antibody directed against the glycoprotein IIb/IIIa complex was added to the platelet suspension. Platelet adhesion to surfaces preadsorbed to von Willebrand factor deficient plasma was the same as to surfaces preadsorbed with normal plasma.While it appears that surface bound fibrinogen does mediate the initial attachment of platelets to Biomer, the observation that the fibrinogen adsorption and platelet adhesion maxima do not coincide exactly also suggests that the degree of subsequent platelet adhesion is dictated not only by the amount of surface bound fibrinogen but also by its conformation.

Fibrinogen adsorption on Pyrex glass tubes: A continuous kinetic study

1992 ◽  
Vol 42 (1) ◽  
pp. 87-92 ◽  
Author(s):  
F. Boumaza ◽  
Ph. Déjardin ◽  
F. Yan ◽  
F. Bauduin ◽  
Y. Holl
Keyword(s):  
Kinetic Study ◽  
Pyrex Glass ◽  
Glass Tubes ◽  
Fibrinogen Adsorption

A look into the interaction of metal oxide thin films with biological media: Albumin and Fibrinogen adsorption

2012 ◽  
Vol 1376 ◽  
Author(s):  
P. Silva-Bermudez ◽  
S. Muhl ◽  
M. Rivera ◽  
S. E. Rodil
Keyword(s):  
Surface Roughness ◽  
Surface Energy ◽  
Protein Adsorption ◽  
Photoelectron Spectroscopy ◽  
Ex Situ ◽  
Polar Component ◽  
Metal Oxide Thin Films ◽  
Adsorbed Protein ◽  
Major Chemical ◽  
Fibrinogen Adsorption

ABSTRACTIn the present work, the adsorption of albumin and fibrinogen on Ta, Nb, Ti and Zr oxidesthin films deposited on Si (100) wafers by magnetron sputtering was studied in order to get a better understanding of the correlation among the surface properties of these oxides and the protein adsorption phenomena on their surfaces. The surface energy, hydrophobicity, chemical composition, roughness and atomic order of the films were characterized. The films were immersedfor 45 minutes in single protein solutions; either albumin or fibrinogenand the adsorbed protein layer on the films was studied ex-situ in a dry ambient using bothX-ray photoelectron spectroscopy and atomic force microscopy.The adsorption of albumin and fibrinogen on the films modified the surface morphology and decreased the surface roughness for all the four different metal oxides. The XPS results confirmed the presence of the protein on the surface of the films and showed that the two proteins studied were adsorbed without undergoing a major chemical decomposition. A correlation between the surface roughness,the polar component of the surface energy of the films and the atomic percentage of nitrogen on the films after protein adsorption, an indirect signal of the amount of protein adsorbed, was found for albumin and fibrinogen adsorption on Ta, Nb and Ti oxides; the largest the roughness or the polar component the largest amount of adsorbed protein.

scholarly journals Excess fibrinogen adsorption to monolayers of mixed lipids

2010 ◽  
Vol 81 (2) ◽  
pp. 607-613 ◽  
Author(s):  
V. Deshmukh ◽  
D.W. Britt ◽  
V. Hlady
Keyword(s):  
Fibrinogen Adsorption

Protein Adsorption on Surface-Attached Weak Polyelectrolyte Layers

2012 ◽  
Vol 621 ◽  
pp. 93-96
Author(s):  
Qiong Li ◽  
Hai Ning Zhang
Keyword(s):  
Protein Adsorption ◽  
Methacrylic Acid ◽  
Electrostatic Interactions ◽  
Adsorbed Amount ◽  
Topmost Layer ◽  
Charge Sign ◽  
Fibrinogen Adsorption

Fibrinogen adsorption onto surface-attached poly(methacrylic acid) monolayers and poly(methacrylic acid)/polyethyleneimine bilayers has been investigated. The adsorbed amount of fibrinogen was found to be dependant on the charge sign of the topmost layer because of electrostatic interactions.

Die Fibrinogen-Adsorption – eine neue Therapieoption des Ulcus cruris venosum?

VASA ◽  
2003 ◽  
Vol 32 (3) ◽  
pp. 173-177 ◽  
Author(s):  
Stücker ◽  
Moll ◽  
Rudolph ◽  
Robak-Pawelczyk ◽  
Jünger ◽  
...  
Keyword(s):  
Case Report ◽  
Adverse Effects ◽  
Surface Area ◽  
Blood Viscosity ◽  
Lower Leg ◽  
Ulcus Cruris Venosum ◽  
Venous Blood Flow ◽  
Venous Ulceration ◽  
Compression Bandages ◽  
Fibrinogen Adsorption

The initial element in the causation of venous ulceration is a disturbance of venous blood flow that leads to an increase in venous pressure. Eventually, however, it is the microcirculatory consequences of venous hypertension that lead to trophic skin changes and finally to ulceration. A reduction in blood viscosity results in an improvement at the microcirculatory level. The elimination of fibrinogen from plasma improves blood viscosity. This case report concerns a 75-year-old woman with venous ulcers of both legs (left lower leg: deep ulceration with a surface area of 3x5 cm; right lower leg: superficial, confluent ulceration with a total surface area of 5x10 cm). The patient underwent 20 sessions of fibrinogen adsorption, while simultaneously continuing with a regimen of conservative measures (activated charcoal cloth dressing with silver, calcium alginate dressings and short-stretch compression bandages). Following binding to a peptide (Gly-Pro-Arg-Pro-Lys), fibrinogen and fibrin were specifically removed from the patient’s plasma: her fibrinogen concentration was lowered from an original mean level of 310 mg/dl (SD ± 104 mg/dl) to 136 mg/dl (SD ± 54 mg/dl), and there was no return to the baseline concentration by the time of the next fibrinogen adsorption session. In response to this treatment the patient’s ulcers healed rapidly within 9 weeks. Dizziness and hematomas at the vascular access sites in both antecubital fossae were reported as adverse effects. A fall in hematocrit was also noted (before treatment 37% ± 1%; after treatment 35% ± 2%). This may have been caused by hemodilution due to the procedure and to cell losses during blood-plasma separation, a phenomenon that is known to occur during apheresis. This case report suggests that fibrinogen adsorption is low in adverse effects and is a useful addition to the range of treatments available for ulcers of venous etiology.

Modification of polymer surfaces: optimization of approaches

Perfusion ◽  
2003 ◽  
Vol 18 (1_suppl) ◽  
pp. 33-39 ◽  
Author(s):  
J M Courtney ◽  
X B Zhao ◽  
H Qian ◽  
A Sharma
Keyword(s):  
Surface Treatment ◽  
Vinyl Chloride ◽  
Cell Membranes ◽  
Clinical Success ◽  
Polymer Surfaces ◽  
Important Means ◽  
Modification Procedure ◽  
Modified Surface ◽  
Fibrinogen Adsorption

Modification of polymer surfaces to achieve a surface with enhanced compatibility is an important means of obtaining improved biomaterials. Techniques are available for altering the hydrophilicity or charge of a surface, attaching macromolecules or attempting to resemble cell membranes. Relevant to the clinical success of a modified surface is the modification procedure and a procedure based on incorporation as opposed to surface treatment has potential advantages. The modification of plasticized vinyl chloride (PVC) by the incorporation of cyclodextrins is described. In comparison to unmodified PVC controls, cyclodextrin incorporation reduced fibrinogen adsorption, with the extent of reduction dependent on the type and quantity of cyclodextrin incorporated.

Reduction of fibrinogen adsorption on PEG-coated polystyrene surfaces

1992 ◽  
Vol 26 (6) ◽  
pp. 779-790 ◽  
Author(s):  
Karin Bergström ◽  
Krister Holmberg ◽  
Agneza Safranj ◽  
Allan S. Hoffman ◽  
Michael J. Edgell ◽  
...  
Keyword(s):  
Fibrinogen Adsorption

scholarly journals Does the conformation of adsorbed fibrinogen dictate platelet interactions with artificial surfaces?

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 355-362 ◽  
Author(s):  
JN Lindon ◽  
G McManama ◽  
L Kushner ◽  
EW Merrill ◽  
EW Salzman
Keyword(s):  
Platelet Adhesion ◽  
Surface Coverage ◽  
Platelet Reactivity ◽  
Polymer Surfaces ◽  
Side Chain ◽  
Alkyl Side Chain ◽  
Fibrinogen Binding ◽  
Artificial Surfaces ◽  
Antibody Preparations ◽  
Fibrinogen Adsorption

Abstract Platelet activation by polymer surfaces is thought to require preliminary adsorption of fibrinogen and perhaps changes in fibrinogen conformation. We measured fibrinogen adsorption by a series of polymers by two methods, using either 125I-labeled fibrinogen or 125I-labeled antifibrinogen antibodies, and correlated the results with platelet reactivity (retention and secretion) in columns of beads coated with the polymers. For polyalkyl methacrylates with 1 to 4 carbon side chains, platelet reactivity varied directly with increasing length of the alkyl side chain and with the quantity of bound fibrinogen recognizable by antifibrinogen antibody but not with the total quantity of fibrinogen adsorbed. The same pattern of results was seen with five antibody preparations, including affinity-purified Fab fragments against the D or E domain of fibrinogen. Tests of platelet retention and fibrinogen binding to four polyalkyl acrylates and to three unrelated polymers (polystyrene, polymethyl methacrylate, and a polyether polyurethane) indicated that platelet retention correlated positively with both total fibrinogen binding and with the amount of antibody-recognizable fibrinogen bound. Drugs that block platelet aggregation, but not adhesion, did not alter the hierarchy of platelet retention to the polyalkyl methacrylates. These data suggest that, contrary to previous views, platelet adhesion to artificial surfaces increases with increasing surface coverage of adsorbed fibrinogen if the bound fibrinogen maintains a conformation such that its functional domains remain recognizable by antibody probes.

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