Streptavidin-coated surfaces suppress bacterial colonization by inhibiting non-specific protein adsorption

2017 ◽  
Vol 106 (3) ◽  
pp. 758-768 ◽  
Author(s):  
Volker Ettelt ◽  
Katharina Ekat ◽  
Peer W. Kämmerer ◽  
Bernd Kreikemeyer ◽  
Matthias Epple ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Bacterial Colonization ◽  
Specific Protein ◽  
Coated Surfaces

scholarly journals Nanogel surface coatings for improved single-molecule imaging substrates

2011 ◽  
Vol 8 (63) ◽  
pp. 1400-1408 ◽  
Author(s):  
Lee A. Tessler ◽  
Casey D. Donahoe ◽  
Daniel J. Garcia ◽  
Young-Shin Jun ◽  
Donald L. Elbert ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Single Molecule ◽  
High Sensitivity ◽  
Specific Protein ◽  
Dna Adsorption ◽  
Lower Protein ◽  
Bioanalytical Applications ◽  
Poly Ethylene ◽  
Coated Surfaces ◽  
Sm Protein

Surfaces that resist protein adsorption are important for many bioanalytical applications. Bovine serum albumin (BSA) coatings and multi-arm poly(ethylene glycol) (PEG) coatings display low levels of non-specific protein adsorption and have enabled highly quantitative single-molecule (SM) protein studies. Recently, a method was developed for coating a glass with PEG–BSA nanogels, a promising hybrid of these two low-background coatings. We characterized the nanogel coating to determine its suitability for SM protein experiments. SM adsorption counting revealed that nanogel-coated surfaces exhibit lower protein adsorption than covalently coupled BSA surfaces and monolayers of multi-arm PEG, so this surface displays one of the lowest degrees of protein adsorption yet observed. Additionally, the nanogel coating was resistant to DNA adsorption, underscoring the utility of the coating across a variety of SM experiments. The nanogel coating was found to be compatible with surfactants, whereas the BSA coating was not. Finally, applying the coating to a real-world study, we found that single ligand molecules could be tethered to this surface and detected with high sensitivity and specificity by a digital immunoassay. These results suggest that PEG–BSA nanogel coatings will be highly useful for the SM analysis of proteins.

A Novel Method to Attenuate Protein Adsorption Using Combinations of Polyethylene Glycol (PEG) Grafts and Piezoelectric Actuation

2010 ◽  
Vol 1 (4) ◽  
Author(s):  
Po-Ying Yeh ◽  
Jayachandran N. Kizhakkedathu ◽  
Mu Chiao
Keyword(s):  
Polyethylene Glycol ◽  
Protein Adsorption ◽  
Lead Zirconate Titanate ◽  
Photoelectron Spectroscopy ◽  
Theoretical Calculations ◽  
Lead Zirconate ◽  
Piezoelectric Composite ◽  
Coated Surfaces ◽  
Bsa Adsorption

An antifouling treatment based on the combined effects of grafted polyethylene glycol (PEG) polymers and the application of vibration is reported. A gold-coated lead zirconate titanate piezoelectric composite was grafted with PEG used as a model substrate. The PEG grafted surfaces were thoroughly characterized by attenuated total reflectance-Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. In vitro protein adsorption onto PEG coated surfaces was studied with and without the application of vibration. Bovine serum albumin (BSA) adsorption onto PEG grafted surfaces followed a similar pattern as reported in literature. However, when piezoelectric vibration was applied on the PEG grafted surface, BSA desorption was observed. At very low graft densities, the vibration significantly reduced the BSA adsorption compared with high PEG graft densities. Theoretical calculations showed that the thickness of PEG layer on the surface was affecting vibration induced protein desorption.

Polyglycerol based coatings to reduce non-specific protein adsorption in sample vials and on SPR sensors

2015 ◽  
Vol 867 ◽  
pp. 47-55 ◽  
Author(s):  
Tobias Becherer ◽  
Christian Grunewald ◽  
Vivienne Engelschalt ◽  
Gerhard Multhaup ◽  
Thomas Risse ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Specific Protein

Counterion-Specific Protein Adsorption on Polyelectrolyte Brushes

Langmuir ◽  
2015 ◽  
Vol 31 (22) ◽  
pp. 6078-6084 ◽  
Author(s):  
Jun Yang ◽  
Zan Hua ◽  
Tao Wang ◽  
Bo Wu ◽  
Guangming Liu ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Specific Protein ◽  
Polyelectrolyte Brushes

Effect of Surface Wettability on Ion-Specific Protein Adsorption

Langmuir ◽  
2012 ◽  
Vol 28 (41) ◽  
pp. 14642-14653 ◽  
Author(s):  
Xiaowen Wang ◽  
Guangming Liu ◽  
Guangzhao Zhang
Keyword(s):  
Protein Adsorption ◽  
Specific Protein ◽  
Surface Wettability ◽  
Effect Of Surface
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