The progress and challenges for dermal regeneration in tissue engineering

2017 ◽  
Vol 105 (4) ◽  
pp. 1208-1218 ◽  
Author(s):  
Hanlei Zhou ◽  
Chuangang You ◽  
Xingang Wang ◽  
Ronghua Jin ◽  
Pan Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Dermal Regeneration

scholarly journals Enhanced stabilization of collagen-based dermal regeneration scaffolds through the combination of physical and chemical crosslinking

2008 ◽  
Vol 104 (11/12) ◽  
Author(s):  
Q.B. Wessels ◽  
E. Pretorius
Keyword(s):  
Tissue Engineering ◽  
Enzymatic Degradation ◽  
Physiochemical Properties ◽  
Biomedical Device ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Dermal Regeneration ◽  
Cytotoxicity Assessment ◽  
Physical And Chemical

The use of collagen in the biomedical device industry has led to major advances in soft tissue repair. This is attributed largely to the favourable biological and physiochemical properties of collagen. Regenerative medicine and tissue engineering favoured the use of this biomaterial and various commercial products have become available in the past few decades. This study aims to develop a collagen and chondroitin-6-sulphate dermal regeneration scaffold with enhanced resistance against enzymatic degradation. Frozen slurries (0.5% collagen) were dried under vacuum, coated with silicone, crosslinked and then thoroughly rinsed. The scaffolds were subjected to a range of quantitative and qualitative tests that included: scanning electron microscopy analysis, collagenase enzymatic degradation, and cytotoxicity assessment. Scaffold resistance to enzymatic degradation was manipulated after dehydrothermal treatment by employing combinations of crosslinking agents, such as glutaraldehyde and/or carbodiimide, with or without the presence of L-lysine. Results indicate that highly porous (mean pore diameter of 87.3 µm), bioactive, non-cytotoxic tissue engineering matrices were obtained. Enhanced stability of these scaffolds was achieved through extensive crosslinking and suggests the potential to prevent in vivo wound contraction sufficiently.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Combination of MRI and 99mTc-NTP 15-5 Scintigraphy for the Assessment of Intervertebral Disk Degeneration and Tissue Engineering

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1319873-s-0032-1319873
Author(s):  
P. Colombier ◽  
J. Clouet ◽  
E. Miot-Noirault ◽  
A. Vidal ◽  
F. Cachin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Intervertebral Disk ◽  
Intervertebral Disk Degeneration ◽  
Disk Degeneration
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