Neurite extension and neuronal differentiation of human induced pluripotent stem cell derived neural stem cells on polyethylene glycol hydrogels containing a continuous Young's Modulus gradient

2016 ◽  
Vol 105 (3) ◽  
pp. 824-833 ◽  
Author(s):  
Matthew C. Mosley ◽  
Hyun Ju Lim ◽  
Jing Chen ◽  
Yueh-Hsun Yang ◽  
Shenglan Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Polyethylene Glycol ◽  
Neural Stem Cells ◽  
Young’S Modulus ◽  
Neuronal Differentiation ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Neurite Extension ◽  
Induced Pluripotent

scholarly journals Targeted Inhibition of the miR-199a/214 Cluster by CRISPR Interference Augments the Tumor Tropism of Human Induced Pluripotent Stem Cell-Derived Neural Stem Cells under Hypoxic Condition

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yumei Luo ◽  
Xuehu Xu ◽  
Xiuli An ◽  
Xiaofang Sun ◽  
Shu Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Stem Cell ◽  
Cell Migration ◽  
Neural Stem Cells ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Tumor Tropism ◽  
Targeted Inhibition ◽  
Induced Pluripotent

The human induced pluripotent stem cell (hiPSC) provides a breakthrough approach that helps overcoming ethical and allergenic challenges posed in application of neural stem cells (NSCs) in targeted cancer gene therapy. However, the tumor-tropic capacity of hiPSC-derived NSCs (hiPS-NSCs) still has much room to improve. Here we attempted to promote the tumor tropism of hiPS-NSCs by manipulating the activity of endogenous miR-199a/214 cluster that is involved in regulation of hypoxia-stimulated cell migration. We first developed a baculovirus-delivered CRISPR interference (CRISPRi) system that sterically blocked the E-box element in the promoter of the miR-199a/214 cluster with an RNA-guided catalytically dead Cas9 (dCas9). We then applied this CRISPRi system to hiPS-NSCs and successfully suppressed the expression of miR-199a-5p, miR-199a-3p, and miR-214 in the microRNA gene cluster. Meanwhile, the expression levels of their targets related to regulation of hypoxia-stimulated cell migration, such as HIF1A, MET, and MAPK1, were upregulated. Further migration assays demonstrated that the targeted inhibition of the miR-199a/214 cluster significantly enhanced the tumor tropism of hiPS-NSCs both in vitro and in vivo. These findings suggest a novel application of CRISPRi in NSC-based tumor-targeted gene therapy.

scholarly journals Retraction of “Glioma Gene Therapy Using Induced Pluripotent Stem Cell Derived Neural Stem Cells”

2019 ◽  
Vol 16 (9) ◽  
pp. 4088-4088
Author(s):  
Esther Xingwei Lee ◽  
Dang Hoang Lam ◽  
Chunxiao Wu ◽  
Jing Yang ◽  
Chee Kian Tham ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent
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