scholarly journals Electroactive polyurethane/siloxane derived from castor oil as a versatile cardiac patch, part I: Synthesis, characterization,and myoblast proliferation and differentiation

2016 ◽  
Vol 104 (6) ◽  
pp. 1570-1570
Author(s):  
Nafiseh Baheiraei ◽  
Reza Gharibi ◽  
Hamid Yeganeh ◽  
Michele Miragoli ◽  
Nicolò Salvarani ◽  
...  
Keyword(s):  
Castor Oil ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation ◽  
Cardiac Patch

scholarly journals MicroRNA-27b-3p Targets the Myostatin Gene to Regulate Myoblast Proliferation and Is Involved in Myoblast Differentiation

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 423
Author(s):  
Genxi Zhang ◽  
Mingliang He ◽  
Pengfei Wu ◽  
Xinchao Zhang ◽  
Kaizhi Zhou ◽  
...  
Keyword(s):  
Muscle Growth ◽  
Luciferase Reporter ◽  
Myoblast Differentiation ◽  
Regulation Mechanism ◽  
Rna Seq ◽  
Myostatin Gene ◽  
Primary Myoblasts ◽  
Chicken Muscle ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation

microRNAs play an important role in the growth and development of chicken embryos, including the regulation of skeletal muscle genesis, myoblast proliferation, differentiation, and apoptosis. Our previous RNA-seq studies showed that microRNA-27b-3p (miR-27b-3p) might play an important role in regulating the proliferation and differentiation of chicken primary myoblasts (CPMs). However, the mechanism of miR-27b-3p regulating the proliferation and differentiation of CPMs is still unclear. In this study, the results showed that miR-27b-3p significantly promoted the proliferation of CPMs and inhibited the differentiation of CPMs. Then, myostatin (MSTN) was confirmed to be the target gene of miR-27b-3p by double luciferase reporter assay, RT-qPCR, and Western blot. By overexpressing and interfering with MSTN expression in CPMs, the results showed that overexpression of MSTN significantly inhibited the proliferation and differentiation of CPMs. In contrast, interference of MSTN expression had the opposite effect. This study showed that miR-27b-3p could promote the proliferation of CPMs by targeting MSTN. Interestingly, both miR-27b-3p and MSTN can inhibit the differentiation of CPMs. These results provide a theoretical basis for further understanding the function of miR-27b-3p in chicken and revealing its regulation mechanism on chicken muscle growth.

scholarly journals CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Xuemei Shen ◽  
Jia Tang ◽  
Rui Jiang ◽  
Xiaogang Wang ◽  
Zhaoxin Yang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Muscle Development ◽  
Inhibitory Effect ◽  
Akt Signaling ◽  
Luciferase Assay ◽  
Circular Rnas ◽  
Akt Signaling Pathway ◽  
Sequencing Data ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation

AbstractMany novel non-coding RNAs, such as microRNAs (miRNAs) and circular RNAs (circRNAs), are involved in various physiological and pathological processes. The PI3K/AKT signaling pathway is important for its role in regulating skeletal muscle development. In this study, molecular and biochemical assays were used to confirm the role of miRNA-145 (miR-145) in myoblast proliferation and apoptosis. Based on sequencing data and bioinformatics analysis, we identified a new circRILPL1, which acts as a sponge for miR-145. The interactions between circRILPL1 and miR-145 were examined by bioinformatics, a luciferase assay, and RNA immunoprecipitation. Mechanistically, knockdown or exogenous expression of circRILPL1 in the primary myoblasts was performed to prove the functional significance of circRILPL1. We investigated the inhibitory effect of miR-145 on myoblast proliferation by targeting IGF1R to regulate the PI3K/AKT signaling pathway. A novel circRILPL1 was identified that could sponge miR-145 and is related to AKT activation. In addition, circRILPL1 was positively correlated with muscle proliferation and differentiation in vitro and could inhibit cell apoptosis. The newly identified circRILPL1 functions as a miR-145 sponge to regulate the IGF1R gene and rescue the inhibitory effect of miR-145 on the PI3K/AKT signaling pathway, thereby promoting myoblast growth.

scholarly journals Gga-miR-205a Affecting Myoblast Proliferation and Differentiation by Targeting CDH11

2018 ◽  
Vol 9 ◽  
Author(s):  
Zhijun Wang ◽  
Hongjia Ouyang ◽  
Xiaolan Chen ◽  
Jiao Yu ◽  
Bahareldin A. Abdalla ◽  
...  
Keyword(s):  
Proliferation And Differentiation ◽  
Myoblast Proliferation

miR-22 regulates C2C12 myoblast proliferation and differentiation by targeting TGFBR1

2018 ◽  
Vol 97 (4) ◽  
pp. 257-268 ◽  
Author(s):  
Han Wang ◽  
Qian Zhang ◽  
BinBin Wang ◽  
WangJun Wu ◽  
Julong Wei ◽  
...  
Keyword(s):  
C2c12 Myoblast ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation

scholarly journals A role for Regulator of G protein Signaling-12 (RGS12) in the balance between myoblast proliferation and differentiation

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0216167 ◽  
Author(s):  
Adam B. Schroer ◽  
Junaith S. Mohamed ◽  
Melinda D. Willard ◽  
Vincent Setola ◽  
Emily Oestreich ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Proliferation And Differentiation ◽  
Myoblast Proliferation

scholarly journals Involvement of ST6Gal I‐mediated α2,6 sialylation in myoblast proliferation and differentiation

FEBS Open Bio ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 56-69 ◽  
Author(s):  
Caroline Vergé ◽  
Amel Bouchatal ◽  
Frédéric Chirat ◽  
Yann Guérardel ◽  
Abderrahman Maftah ◽  
...  
Keyword(s):  
Proliferation And Differentiation ◽  
Myoblast Proliferation ◽  
St6gal I
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