Chondrogenic potential of physically treated bovine cartilage matrix derived porous scaffolds on human dermal fibroblast cells

2015 ◽  
Vol 104 (1) ◽  
pp. 245-256 ◽  
Author(s):  
Ali Moradi ◽  
Forough Ataollahi ◽  
Katayoun Sayar ◽  
Sumit Pramanik ◽  
Pan-Pan Chong ◽  
...  
Keyword(s):  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Cartilage Matrix ◽  
Porous Scaffolds ◽  
Fibroblast Cells ◽  
Chondrogenic Potential ◽  
Bovine Cartilage

scholarly journals The Effect of Parietin Isolated From Rheum ribes L on In Vitro Wound Model Using Human Dermal Fibroblast Cells

2019 ◽  
Vol 18 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Gulsah Gundogdu ◽  
Koksal Gundogdu ◽  
Kemal Alp Nalci ◽  
Alper Kursat Demirkaya ◽  
Seymanur Yılmaz Tascı ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Dose Range ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cells ◽  
Wound Model ◽  
Dpph Method ◽  
Pipette Tip

Parietin is one of the well-known anthraquinone compounds that can be extracted from Rheum ribes L. In this study, we aimed to investigate the effects of parietin isolated from Rheum ribes L on an in vitro wound model using human dermal fibroblast cells and compare its effectiveness against zinc. The antioxidant effect of parietin was determined by using the 1,1-diphenyl-2-picrylhydrazine (DPPH) method. Human dermal fibroblast cells were cultured in proculture medium and were kept until 100% confluence was achieved. The wound model was created by using a pipette tip. After that, different concentrations of parietin and zinc (final concentrations in the well to be 5-250 µM and 25-200 µM, respectively) were added into the medium. The proliferation-inducing effect on cell viability was determined by using MTT assay. Images of cells were taken at 0, 12, and 24 hours. According to the DPPH method, parietin exhibited have antioxidant activity. According to the MTT results, parietin exhibited significant proliferation-inducing effect on cell viability in a dose range of 5 to 10 M, and zinc showed significant proliferation-inducing effect on cell viability at dose 50 µM ( P < .05). In addition, the image of cell proliferation was also shown at the same doses at 24 hours. In this study, we claim that parietin induces cell proliferation at low doses in cases of dermal fibroblast loss. In conclusion, parietin as an alternative to zinc in wound healing could be used by clinicians in the future with more extensive studies.

Modulation of heat shock protein 90 affects TGF-β-induced collagen synthesis in human dermal fibroblast cells

2016 ◽  
Vol 48 (6) ◽  
pp. 616-623 ◽  
Author(s):  
Sae Bin Lee ◽  
A-ram Lim ◽  
Dong Kyun Rah ◽  
Kyung Soo Kim ◽  
Hyun Jin Min
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Collagen Synthesis ◽  
Dermal Fibroblast ◽  
Heat Shock Protein 90 ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cells

Radioprotective role of uric acid: evidence from studies in Drosophila and human dermal fibroblast cells

2020 ◽  
Vol 47 (4) ◽  
pp. 2427-2436 ◽  
Author(s):  
Jagdish Gopal Paithankar ◽  
Avinash Kundadka Kudva ◽  
Shamprasad Varija Raghu ◽  
Rajashekhar K. Patil
Keyword(s):  
Uric Acid ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Fibroblast Cells
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