Graft of the gelatin sponge scaffold containing genetically-modified neural stem cells promotes cell differentiation, axon regeneration, and functional recovery in rat with spinal cord transection

2014 ◽  
Vol 103 (4) ◽  
pp. 1533-1545 ◽  
Author(s):  
Bao-Ling Du ◽  
Xiang Zeng ◽  
Yuan-Huan Ma ◽  
Bi-Qin Lai ◽  
Jun-Mei Wang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Cell Differentiation ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Axon Regeneration ◽  
Genetically Modified ◽  
Spinal Cord Transection ◽  
Gelatin Sponge

Functional Recovery Following Spinal Cord Hemisection Mediated by a Unique Polymer Scaffold Seeded with Neural Stem Cells

2000 ◽  
Vol 662 ◽  
Author(s):  
Erin Lavik ◽  
Yang D. Teng ◽  
David Zurakowski ◽  
Xianlu Qu ◽  
Evan Snyder ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Axonal Guidance ◽  
General Character ◽  
Cord Injury ◽  
Scaffold Structure ◽  
Spinal Cord Hemisection

AbstractA dual scaffold structure made of biodegradable polymers and seeded with neural stem cells has been developed to address the issues of spinal cord injury including axonal severance and the loss of neurons and glia. The general design of the scaffold is derived the structure of the spinal cord with an outer section which mimics the white matter with long axial pores to provide axonal guidance and an inner section seeded with neural stem cells to address the issues of cell replacement and mimic the general character of the gray matter. The seeded scaffold leads to improved functional recovery as compared with the lesion control or cells alone following spinal cord injury.

scholarly journals Pre-Evaluated Safe Human iPSC-Derived Neural Stem Cells Promote Functional Recovery after Spinal Cord Injury in Common Marmoset without Tumorigenicity

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52787 ◽  
Author(s):  
Yoshiomi Kobayashi ◽  
Yohei Okada ◽  
Go Itakura ◽  
Hiroki Iwai ◽  
Soraya Nishimura ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Common Marmoset ◽  
Cord Injury ◽  
Human Ipsc

scholarly journals Therapeutic Effect of BDNF-Overexpressing Human Neural Stem Cells (F3.BDNF) in a Contusion Model of Spinal Cord Injury in Rats

2021 ◽  
Vol 22 (13) ◽  
pp. 6970
Author(s):  
Da-Jeong Chang ◽  
Hwi-Young Cho ◽  
Seyoung Hwang ◽  
Nayeon Lee ◽  
Chunggab Choi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Inflammatory Cells ◽  
Thoracic Spinal Cord ◽  
Human Neural Stem Cells ◽  
Neural Lineage ◽  
Cord Injury

The most common type of spinal cord injury is the contusion of the spinal cord, which causes progressive secondary tissue degeneration. In this study, we applied genetically modified human neural stem cells overexpressing BDNF (brain-derived neurotrophic factor) (F3.BDNF) to determine whether they can promote functional recovery in the spinal cord injury (SCI) model in rats. We transplanted F3.BDNF cells via intrathecal catheter delivery after a contusion of the thoracic spinal cord and found that they were migrated toward the injured spinal cord area by MR imaging. Transplanted F3.BDNF cells expressed neural lineage markers, such as NeuN, MBP, and GFAP and were functionally connected to the host neurons. The F3.BDNF-transplanted rats exhibited significantly improved locomotor functions compared with the sham group. This functional recovery was accompanied by an increased volume of spared myelination and decreased area of cystic cavity in the F3.BDNF group. We also observed that the F3.BDNF-transplanted rats showed reduced numbers of Iba1- and iNOS-positive inflammatory cells as well as GFAP-positive astrocytes. These results strongly suggest the transplantation of F3.BDNF cells can modulate inflammatory cells and glia activation and also improve the hyperalgesia following SCI.

scholarly journals Swimming kinematics and performance of spinal transected lampreys with different levels of axon regeneration

2021 ◽  
Author(s):  
Jacob Fies ◽  
Brad J. Gemmell ◽  
Stephanie M. Fogerson ◽  
Jennifer R. Morgan ◽  
Eric D. Tytell ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Functional Recovery ◽  
Axon Regeneration ◽  
Swimming Performance ◽  
High Amplitude ◽  
Body Waves ◽  
Spinal Cord Transection ◽  
Neural Function ◽  
Lower Amplitude ◽  
Cord Injury

Axon regeneration is critical for restoring neural function after spinal cord injury. This has prompted a series of studies on the neural and functional recovery of lampreys after spinal cord transection. Despite this, there are still many basic questions remaining about how much functional recovery depends on axon regeneration. Our goal was to examine how swimming performance was related to degree of axon regeneration in lampreys recovering from spinal cord transection by quantifying the relationship between swimming performance and percent axon regeneration of transected lampreys after 11 weeks of recovery. We found that while swimming speeds varied, they did not relate to percent axon regeneration. In fact, swimming speeds were highly variable within individuals meaning that most individuals could swim at both moderate and slow speeds, regardless of percent axon regeneration. However, none of the transected individuals were able to swim as fast as the control lampreys. To swim fast, control lamprey generated high amplitude body waves with long wavelengths. Transected lampreys generated body waves with lower amplitude and shorter wavelengths than controls and to compensate, transected lamprey increased their wave frequencies to swim faster. As a result, transected lampreys had significantly higher frequencies than control lamprey at comparable swimming velocities. These data suggest that the control lampreys swam more efficiently than transected lampreys. In conclusion, there appears to be a minimal recovery threshold in terms of percent axon regeneration required for lampreys to be capable of swimming, however, there also seems to be a limit to how much they can behaviorally recover.

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