Adapted chondrogenic differentiation of human mesenchymal stem cells via controlled release of TGF-β1 from poly(ethylene oxide)-terephtalate/poly(butylene terepthalate) multiblock scaffolds

2014 ◽  
Vol 103 (1) ◽  
pp. 371-383 ◽  
Author(s):  
Ana Rey-Rico ◽  
Jagadeesh K. Venkatesan ◽  
Jerome Sohier ◽  
Lorenzo Moroni ◽  
Magali Cucchiarini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Controlled Release ◽  
Ethylene Oxide ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Tgf Β1

Effect of Surface Electrostatic Property on Chondrogenic Differentiation of Human Mesenchymal Stem Cells

2007 ◽  
Vol 330-332 ◽  
pp. 1189-1192
Author(s):  
Li Guo ◽  
Yu Jiang Fan ◽  
Naoki Kawazoe ◽  
Guo Ping Chen ◽  
M. Tanaka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Acrylic Acid ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Fast Green ◽  
Reactive Polymers ◽  
Poly Ethylene ◽  
Safranin O

In this study, three kinds of photo-reactive polymers, namely positively charged azidophenyl-derived polyallylamine (AzPhPAAm), negatively charged azidophenyl-derived poly(acrylic acid) (AzPhPAAc) and neutral azidophenyl-derived poly(ethylene glycol) (AzPhPEG), were synthesized by introduction of photo-reactive group (-N3) into polyallylamine, poly(acrylic acid) and poly(ethylene glycol), respectively. The structure of the azidophenyl-derived polymers was confirmed by 1H-NMR measurement. All of the photo-reactive polymers can be pattern-grafted on the surface of cells culture plate, which was confirmed by the optical microscopy observation. The contact angle decreased after surface modification by the photo-reactive polymers. Human mesenchymal stem cells (MSC) cultured on the modified surfaces showed different morphology. The cells adhered and spread more on the PAAm- and PAAc-grafted surfaces than on the PEG-grafted surface. The pellets formed on PAAm- and PEG-grafted surfaces in condrogenic differentiation medium were positively stained by safranin O/ fast green. But the pellet formed on PAAc-grafted surface did not show obviously positive staining for safranin O/ fast green. These results indicate that the PAAm- and PEG-grafted surfaces promoted chondrogenic differentiation of MSC.

Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage

Biomaterials ◽  
2010 ◽  
Vol 31 (28) ◽  
pp. 7298-7307 ◽  
Author(s):  
Shao Qiong Liu ◽  
Quan Tian ◽  
James L. Hedrick ◽  
James Hoi Po Hui ◽  
Pui Lai Rachel Ee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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