The stimulation of osteogenic differentiation of mesenchymal stem cells and vascular endothelial growth factor secretion of endothelial cells by β-CaSiO3/β-Ca3(PO4)2scaffolds

2013 ◽  
Vol 102 (7) ◽  
pp. 2096-2104 ◽  
Author(s):  
Chen Wang ◽  
Kaili Lin ◽  
Jiang Chang ◽  
Jiao Sun
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Factor Secretion ◽  
Growth Factor Secretion ◽  
Stimulation Of

Stromal-Derived Factor and Vascular Endothelial Growth Factor Synergistically Induce Mesenchymal Stem Cells To Differentiate into Endothelial Cells in Hypoxia Condition, which Incorporated Tumor Vasculogenesis and Angiogenesis.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2575-2575
Author(s):  
Gexiu Liu ◽  
Dongmei He ◽  
Yanhui Wu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Vascular Endothelial ◽  
Positive Ratio ◽  
Vasculogenesis And Angiogenesis ◽  
Endothelial Growth Factor

Abstract INTRODUCTION: High level of vascular endothelial growth factor (VEGF) and stromal-derived factor (SDF-1) are often expressed in many carcinoma tissues that are accompanied by intense neovascularization. VEGF is a key regulator of blood vessel formation during both vasculogenesis and angiogenesis. Mesenchymal stem cells (MSCs) in peripheral blood that are released from the bone marrow can be strongly attracted by SDF-1. Low oxygen tension is thought to be an integral component of the tumor microenvironment. To examine tumor angiogenesis in the tumor microenvironment, we studied that SDF-1 and VEGF both can induce MSCs to differentiate into endothelial cells (ECs) in hypoxia condition. METHODS: Adult Sprague-Dawley rat bone marrow MSCs were purified and maintained in DMEM/F12 containing 10% FBS, and were characterized by high expression of CD44, the lack of CD45 and CD11b molecules, their typical spindle-shaped morphology, together with their ability to differentiate into osteogenic, chondrogenic, and adipogenic cells. Subconfluent MSCs were used, were maintained with DMEM/F12 containing 10% FBS in hypoxia condition (93% N2 + 2% O2 +5% CO2), and were treated with or without VEGF and/or SDF-1. During induction, cell morphology, the vascular endothelial markers VE-cadherin and von Willebrand factor (vWF), and mesenchymal cell marker CD44 were observed. After induction, functions of cells were examined. RESULTS: MSCs treated with both VEGF and SDF-1 for 7 days in hypoxic condition changed morphologically and adopted a caudate appearance with rod-shaped microtubulated structures resembling Weibel-Palade bodies, and formed mature endothelial cell tubules in Matrigel(R) cultures after 15 days. Almost every cell expressed VE-cadherin and vWF [(98.32±1.39)%], but CD44 expression was markedly down-regulated. Functional characteristics of vWF release upon histamine stimulation in response to hypoxia were indistinguishable between the MSC-derived endothelial-like cells and cultured mature ECs. The best condition for the differentiation was the combination of 0.3 nM VEGF and 100 ng/ml SDF-1 at a culture duration of 10 to 15 days. However, the positive ratio of both VE-cadherin and vWF in cells induced by different concentration of SDF-1 alone or VEGF alone was lower [(8.35±2.89)% and (27.33±3.26)%, respectively]. The positive ratio of both VE-cadherin and vWF in cells induced by the best combination of 0.3 nM VEGF and 100 ng/ml SDF-1 in normoxic culture condition was (34.91±3.47)%. Finally, in xenogenic transplantation studies using a SCID mouse model, non-small cell lung cancer cells were injected subcutaneously with the MSC-derived endothelial-like cells induced by the best condition. As a result, co-transplantation of the cells promoted the formation of blood vessels. Immunohistochemistry and FISH analysis showed that the MSC-derived cells incorporated tumor vasculogenesis and angiogenesis. CONCLUSION: Stromal-derived factor and vascular endothelial growth factor synergistically induce bone marrow-derived mesenchymal stem cells to differentiate into endothelial cells in hypoxia condition, which incorporated tumor vasculogenesis and angiogenesis. These results suggest that targeting MSCs-derived angiogenesis might be a novel efficient antiangiogenesis strategy to treat cancer.

scholarly journals Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

2021 ◽  
Author(s):  
Kamil Wartalski ◽  
Gabriela Gorczyca ◽  
Jerzy Wiater ◽  
Zbigniew Tabarowski ◽  
Małgorzata Duda
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Primary Component ◽  
Marker Genes ◽  
Vascular Endothelial ◽  
Ovarian Cortex ◽  
Endothelial Growth Factor

AbstractEndothelial cells (ECs), the primary component of the vasculature, play a crucial role in neovascularization. However, the number of endogenous ECs is inadequate for both experimental purposes and clinical applications. Porcine ovarian putative stem cells (poPSCs), although not pluripotent, are characterized by great plasticity. Therefore, this study aimed to investigate whether poPSCs have the potential to differentiate into cells of endothelial lineage. poPSCs were immunomagnetically isolated from postnatal pig ovaries based on the presence of SSEA-4 protein. Expression of mesenchymal stem cells (MSCs) markers after pre-culture, both at the level of mRNA: ITGB1, THY, and ENG and corresponding protein: CD29, CD90, and CD105 were significantly higher compared to the control ovarian cortex cells. To differentiate poPSCs into ECs, inducing medium containing vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), ascorbic acid, and heparin was applied. After 14 days, poPSC differentiation into ECs was confirmed by immunofluorescence staining for vascular endothelial cadherin (VECad) and vascular endothelial growth factor receptor-2 (VEGFR-2). Semi-quantitative WB analysis of these proteins confirmed their high abundance. Additionally, qRT-PCR showed that mRNA expression of corresponding marker genes: CDH5, KDR was significantly higher compared with undifferentiated poPSCs. Finally, EC functional status was confirmed by the migration test that revealed that they were capable of positive chemotaxis, while tube formation assay demonstrated their ability to develop capillary networks. In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document