An artificial fusion protein between bone morphogenetic protein 2 and titanium-binding peptide is functional in vivo

2013 ◽  
Vol 102 (4) ◽  
pp. 1180-1186 ◽  
Author(s):  
Kazuaki Yuasa ◽  
Eitoyo Kokubu ◽  
Katsutoshi Kokubun ◽  
Kenichi Matsuzaka ◽  
Kiyotaka Shiba ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Binding Peptide ◽  
Morphogenetic Protein

scholarly journals In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

2016 ◽  
Vol 5 (6) ◽  
pp. 730-742 ◽  
Author(s):  
Salwa Suliman ◽  
Yang Sun ◽  
Torbjorn O. Pedersen ◽  
Ying Xue ◽  
Joachim Nickel ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Host Response ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

Immediate versus delayed application of bone morphogenetic protein-2 solution in damaged extraction sockets: a preclinical in vivo investigation

2020 ◽  
Vol 25 (1) ◽  
pp. 275-282
Author(s):  
Myong Ji Kim ◽  
Jae-Kook Cha ◽  
Kyeong-Won Paeng ◽  
Young Woo Song ◽  
Daniel S. Thoma ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals Surface Immobilization of TiO2 Nanotubes with Bone Morphogenetic Protein-2 Synergistically Enhances Initial Preosteoblast Adhesion and Osseointegration

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Ying Li ◽  
Yunjia Song ◽  
Aobo Ma ◽  
Changyi Li
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Surface Immobilization ◽  
Implant Surface ◽  
Cytoskeleton Organization ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Collagen Type 1

Although titanium (Ti) alloys have been widely used as implant materials, the bioinertness of pristine Ti impairs their bioactivity and early osseointegration. In the present work, we prepared TiO2 nanotubes (TNT) layer on the titanium (Ti) surface by anodic oxidation. The anodized surface was functionalized with human bone morphogenetic protein-2 coating to form the hBMP-2/TNT surface. The release behavior of hBMP-2 on the hBMP-2/TNT surface displayed a controlled and sustained pattern, compared to that on the hBMP-2/Ti surface, which showed a rapid release. In vitro cellular activity tests demonstrated that both TNT and hBMP-2/Ti surfaces, particularly the hBMP-2/TNT surface, enhanced adhesion, proliferation, and differentiation of osteoblast cells. Increased cell adhesion, improved cytoskeleton organization, and immunofluorescence staining of vinculin were observed on the modified surfaces. The TNT, hBMP-2/Ti, and hBMP-2/TNT surfaces, especially the hBMP-2/TNT surface, further displayed an upregulated gene expression of adhesion and osteogenic markers vinculin, collagen type 1, osteopontin, and osteocalcin, compared to the pristine Ti surface. In vivo experiments using a rat model demonstrated that the TNT and hBMP-2/Ti surfaces, in particular the hBMP-2/TNT surface, improved osseointegration and showed a superior bone bonding ability compared to Ti. Our study revealed a synergistic role played by TiO2 nanotubes nanotopography and hBMP-2 in promoting initial osteoblast adhesion, proliferation, differentiation, and osseointegration, thus suggesting a promising method for better modifying the implant surface.

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