Directed osteogenic differentiation of human mesenchymal stem/precursor cells on silicate substituted calcium phosphate

2012 ◽  
Vol 101A (1) ◽  
pp. 13-22 ◽  
Author(s):  
Kate Cameron ◽  
Paul Travers ◽  
Chaman Chander ◽  
Tom Buckland ◽  
Charlie Campion ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Precursor Cells

Anionic diketopiperazine induces osteogenic differentiation and supports osteogenesis in a 3D cryogel microenvironment

2021 ◽  
Author(s):  
Apurva Panjla ◽  
Irfan Qayoom ◽  
Ashok Kumar ◽  
Sandeep Verma
Keyword(s):  
Tissue Engineering ◽  
Amino Acid ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Precursor Cells ◽  
Bioactive Molecules ◽  
Osteogenic Potential

Bioactive molecules that enhance or induce osteogenic potential of bone precursor cells have shown vital roles in bone tissue engineering. Herein, we report a novel diketopiperazine, containing taurine amino acid,...

Calcium phosphate-phosphorylated adenosine hybrid microspheres for anti-osteosarcoma drug delivery and osteogenic differentiation

Biomaterials ◽  
2017 ◽  
Vol 121 ◽  
pp. 1-14 ◽  
Author(s):  
Zi-Fei Zhou ◽  
Tuan-Wei Sun ◽  
Feng Chen ◽  
Dong-Qing Zuo ◽  
Hong-Sheng Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation

RNA Synthesis by DNA Methyltransferase 1 siRNA Transfection with Cationic Liposomes into Osteoblastic Progenitor Cells Based on SiO2 Nanomagnetic Beads for Proliferation and Differentiation

2020 ◽  
Vol 20 (10) ◽  
pp. 6077-6086
Author(s):  
Qingzhen Chen ◽  
Tao Jiang ◽  
Qinshen Wang ◽  
Yongqing Huang ◽  
Min Shao
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Osteogenic Differentiation ◽  
Dna Methyltransferase ◽  
Cationic Liposomes ◽  
Precursor Cells ◽  
Dna Methyltransferase 1 ◽  
Alizarin Red ◽  
Alp Activity ◽  
Proliferation And Differentiation

DNA methylation regulated gene expression is important for osteoblast proliferation and differentiation during bone remodeling and its deregulation leads to the development of osteoporosis. DNA methyltransferase 1 (DNMT1) is an important regulator of DNA methylation. To explore the effect and mechanism of differential expression of DNMT1 in osteoblast precursor cells, DNMT1 siRNAs were designed and synthesized to interfere with DNMT1 expression in the osteoblast precursor cells, MC3T3E1 (Clone 24; MC3T3E1-24). The expression of the target gene, DNMT1, and osteogenic differentiation indicators osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB). MTT assay was used to detect the effect on cell proliferation. Alkaline phosphatase (ALP) activity and alizarin red staining were used to detect the effect of DNMT1 on osteogenic differentiation. Hematoxylin and eosin (H&E) staining was used to detect the morphological changes in MC3T3E1-24 cells. Twenty-four hours following the transfection of MC3T3E1-24 cells with DNMT1 siRNA using cationic liposomes, DNMT1 mRNA and protein levels decreased significantly (P <0.001 for both). The reduced expression of DNMT1 promoted the OPG mRNA and protein expression (P <0.05), increased the ratio of OPG to RANKL (P <0.05), inhibited the expression of RANKL (P <0.01) without affecting the RANKL gene expression (not significant, P >0.05). The reduced expression of DNMT1 also promoted the proliferation of osteoblast precursor cells. In addition, ALP activity test and alizarin red staining showed that reduced expression of DNMT1 resulted in an increase in OPG/RANKL ratio and promoted the differentiation of the precursor cells. The cultured cells were found to have fibroblast-like appearance, and calcium nodules were observed after 7 days of conventional culture. In addition, to improve the efficiency of RNA extraction and save time, a type of silica nanomagnetic beads was used in the early stage of this study to extract RNA and assist qPCR detection of the target genes. The results showed that the magnetic beads could effectively extract RNA from the cells. In conclusion, low expression of DNMT1 affects proliferation and maturation of osteoblasts by upregulating OPG and OPG/RANKL ratio.

scholarly journals Glutamine-chitosan modified calcium phosphate nanoparticles for efficient siRNA delivery and osteogenic differentiation

2015 ◽  
Vol 3 (31) ◽  
pp. 6448-6455 ◽  
Author(s):  
Bogyu Choi ◽  
Zhong-Kai Cui ◽  
Soyon Kim ◽  
Jiabing Fan ◽  
Benjamin M. Wu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Gene Transfection ◽  
Sirna Delivery ◽  
Calcium Phosphate Nanoparticles

CaP nanoparticles coated with highly cationic, glutamine-conjugated oligochitosan (Gln-OChi) are developed for siRNA delivery to significantly enhance gene transfection and knockdown efficiency with minimal cytotoxicity. This new nanocarrier can potentially be used for gene therapy.

Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 401-409 ◽  
Author(s):  
Nora Hild ◽  
Oliver D. Schneider ◽  
Dirk Mohn ◽  
Norman A. Luechinger ◽  
Fabian M. Koehler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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