Chitosan/collagen scaffold containing bone morphogenetic protein‐7 DNA supports dental pulp stem cell differentiation in vitro and in vivo

2012 ◽  
Vol 108 (12) ◽  
pp. 2519-2526 ◽  
Author(s):  
Xuechao Yang ◽  
Guangli Han ◽  
Xin Pang ◽  
Mingwen Fan
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Dental Pulp ◽  
Collagen Scaffold ◽  
Stem Cell Differentiation ◽  
Bone Morphogenetic Protein 7 ◽  
Morphogenetic Protein ◽  
Dental Pulp Stem Cell

scholarly journals Macrophage modulation of dental pulp stem cell activity during tertiary dentinogenesis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Vitor C. M. Neves ◽  
Val Yianni ◽  
Paul T. Sharpe
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Immune Cells ◽  
Dental Pulp ◽  
Cell Activation ◽  
Cell Activity ◽  
Stem Cell Differentiation ◽  
Dental Pulp Stem Cell ◽  
Anti Inflammatory

AbstractThe interaction between immune cells and stem cells is important during tissue repair. Macrophages have been described as being crucial for limb regeneration and in certain circumstances have been shown to affect stem cell differentiation in vivo. Dentine is susceptible to damage as a result of caries, pulp infection and inflammation all of which are major problems in tooth restoration. Characterising the interplay between immune cells and stem cells is crucial to understand how to improve natural repair mechanisms. In this study, we used an in vivo damage model, associated with a macrophage and neutrophil depletion model to investigate the role of immune cells in reparative dentine formation. In addition, we investigated the effect of elevating the Wnt/β-catenin pathway to understand how this might regulate macrophages and impact upon Wnt receiving pulp stem cells during repair. Our results show that macrophages are required for dental pulp stem cell activation and appropriate reparative dentine formation. In addition, pharmacological stimulation of the Wnt/β-catenin pathway via GSK-3β inhibitor small molecules polarises macrophages to an anti-inflammatory state faster than inert calcium silicate-based materials thereby accelerating stem cell activation and repair. Wnt/β-catenin signalling thus has a dual role in promoting reparative dentine formation by activating pulp stem cells and promoting an anti-inflammatory macrophage response.

scholarly journals Human adipose stem cell differentiation is highly affected by cancer cells both in vitro and in vivo: implication for autologous fat grafting

2017 ◽  
Vol 8 (1) ◽  
pp. e2568-e2568 ◽  
Author(s):  
Francesca Paino ◽  
Marcella La Noce ◽  
Diego Di Nucci ◽  
Giovanni Francesco Nicoletti ◽  
Rosa Salzillo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Fat Grafting ◽  
Adipose Stem Cell ◽  
Autologous Fat Grafting ◽  
Autologous Fat ◽  
Human Adipose Stem Cell

Photobiomodulatory effect delivered by low-level laser on dental pulp stem cell differentiation for osteogenic lineage

2019 ◽  
Vol 3 (3) ◽  
pp. 175-181 ◽  
Author(s):  
Trivandrum T. Sivakumar ◽  
Alex M. Muruppel ◽  
Anna P. Joseph ◽  
A. Reshmi ◽  
Rajesh Ramachandran ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Dental Pulp ◽  
Stem Cell Differentiation ◽  
Low Level ◽  
Low Level Laser ◽  
Osteogenic Lineage ◽  
Level Laser ◽  
Dental Pulp Stem Cell

scholarly journals Functions of Circular RNAs in Regulating Adipogenesis of Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Fanglin Wang ◽  
Xiang Li ◽  
Zhiyuan Li ◽  
Shoushuai Wang ◽  
Jun Fan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Circular Rna ◽  
Circular Rnas ◽  
Differentiation Process

The mesenchymal stem cells (MSCs) are known as highly plastic stem cells and can differentiate into specialized tissues such as adipose tissue, osseous tissue, muscle tissue, and nervous tissue. The differentiation of mesenchymal stem cells is very important in regenerative medicine. Their differentiation process is regulated by signaling pathways of epigenetic, transcriptional, and posttranscriptional levels. Circular RNA (circRNA), a class of noncoding RNAs generated from protein-coding genes, plays a pivotal regulatory role in many biological processes. Accumulated studies have demonstrated that several circRNAs participate in the cell differentiation process of mesenchymal stem cells in vitro and in vivo. In the current review, characteristics and functions of circRNAs in stem cell differentiation will be discussed. The mechanism and key role of circRNAs in regulating mesenchymal stem cell differentiation, especially adipogenesis, will be reviewed and discussed. Understanding the roles of these circRNAs will present us with a more comprehensive signal path network of modulating stem cell differentiation and help us discover potential biomarkers and therapeutic targets in clinic.

In vitro and in vivo study of human amniotic fluid-derived stem cell differentiation into myogenic lineage

2009 ◽  
Vol 10 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Jean Gekas ◽  
Guillaume Walther ◽  
Daniel Skuk ◽  
Emmanuel Bujold ◽  
Isabelle Harvey ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Amniotic Fluid ◽  
Stem Cell Differentiation ◽  
In Vivo Study ◽  
Human Amniotic Fluid ◽  
Myogenic Lineage

scholarly journals Decellularized Extracellular Matrix as anIn VitroModel to Study the Comprehensive Roles of the ECM in Stem Cell Differentiation

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Takashi Hoshiba ◽  
Guoping Chen ◽  
Chiho Endo ◽  
Hiroka Maruyama ◽  
Miyuki Wakui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Regenerative Medicine ◽  
Intracellular Signaling ◽  
Complex Structure ◽  
Stem Cell Differentiation

Stem cells are a promising cell source for regenerative medicine. Stem cell differentiation must be regulated for applications in regenerative medicine. Stem cells are surrounded by extracellular matrix (ECM)in vivo. The ECM is composed of many types of proteins and glycosaminoglycans that assemble into a complex structure. The assembly of ECM molecules influences stem cell differentiation through orchestrated intracellular signaling activated by many ECM molecules. Therefore, it is important to understand the comprehensive role of the ECM in stem cell differentiation as well as the functions of the individual ECM molecules. Decellularized ECM is a usefulin vitromodel for studying the comprehensive roles of ECM because it retains a native-like structure and composition. Decellularized ECM can be obtained fromin vivotissue ECM or ECM fabricated by cells culturedin vitro. It is important to select the correct decellularized ECM because each type has different properties. In this review, tissue-derived and cell-derived decellularized ECMs are compared asin vitroECM models to examine the comprehensive roles of the ECM in stem cell differentiation. We also summarize recent studies using decellularized ECM to determine the comprehensive roles of the ECM in stem cell differentiation.

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