scholarly journals Erratum: Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes

2011 ◽  
Vol 98A (2) ◽  
pp. 321-321
Author(s):  
Cristina Paternolli ◽  
Martin Neebe ◽  
Enrico Stura ◽  
Francesca Barbieri ◽  
Paola Ghisellini ◽  
...  
Author(s):  
A. Tonosaki ◽  
M. Yamasaki ◽  
H. Washioka ◽  
J. Mizoguchi

A vertebrate disk membrane is composed of 40 % lipids and 60 % proteins. Its fracture faces have been classed into the plasmic (PF) and exoplasmic faces (EF), complementary with each other, like those of most other types of cell membranes. The hypothesis assuming the PF particles as representing membrane-associated proteins has been challenged by serious questions if they in fact emerge from the crystalline formation or decoration effects during freezing and shadowing processes. This problem seems to be yet unanswered, despite the remarkable case of the purple membrane of Halobacterium, partly because most observations have been made on the replicas from a single face of specimen, and partly because, in the case of photoreceptor membranes, the conformation of a rhodopsin and its relatives remains yet uncertain. The former defect seems to be partially fulfilled with complementary replica methods.


1979 ◽  
Vol 88 (2) ◽  
pp. 856-858 ◽  
Author(s):  
V. E. Kagan ◽  
V. Z. Lankin ◽  
A. A. Shvedova ◽  
K. N. Novikov ◽  
S. K. Dobrina ◽  
...  

FEBS Letters ◽  
2001 ◽  
Vol 493 (2-3) ◽  
pp. 112-116 ◽  
Author(s):  
Joachim Bentrop ◽  
Markus Schillo ◽  
Gabriele Gerdon ◽  
Kazimir Draslar ◽  
Reinhard Paulsen

1974 ◽  
Vol 63 (2) ◽  
pp. 587-598 ◽  
Author(s):  
Izhak Nir ◽  
Michael O. Hall

The structure of lipid-depleted retinal rod photoreceptor membranes was studied by means of electron microscopy. Aldehyde-fixed retinas were exhaustively extracted with acetone, chloroform-methanol, and acidified chloroform-methanol. The effect of prefixation on the extractability of lipids was evaluated by means of thin-layer chromatography and fatty acid analysis. Prefixation with glutaraldehyde rendered 38% of the phospholipids unextractable, while only 7% were unextractable after formaldehyde fixation. Embedding the retina in a lipid-retaining, polymerizable glutaraldehyde-urea mixture allows a comparison of the interaction of OsO4 with lipid-depleted membranes and rod disk membranes which contain all their lipids. A decrease in electron density and a deterioration of membrane fine structure in lipid-depleted tissue are correlated with the extent of lipid extraction. These observations are indicative of the role of the lipid bilayer in the ultrastructural visualization of membrane structure with OsO4. Negatively stained thin sections of extracted tissue reveal substructures in the lipid-depleted rod membranes. These substructures are probably the opsin molecules which are the major protein component of retinal rod photoreceptor membranes.


Nature ◽  
1972 ◽  
Vol 236 (5345) ◽  
pp. 313-314 ◽  
Author(s):  
A. E. BLAUROCK ◽  
M. H. F. WILKINS

1974 ◽  
pp. 329-330
Author(s):  
F. J. M. Daemen ◽  
P. J. G. M. Breugel ◽  
S. L. Bonting

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