Aligned 3D human aortic smooth muscle tissue via layer by layer technique inside microchannels with novel combination of collagen and oxidized alginate hydrogel

2011 ◽  
Vol 98A (2) ◽  
pp. 235-244 ◽  
Author(s):  
Shahrzad Rayatpisheh ◽  
Yin Fun Poon ◽  
Ye Cao ◽  
Jie Feng ◽  
Vincent Chan ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscle Tissue ◽  
Layer By Layer ◽  
Alginate Hydrogel ◽  
Smooth Muscle Tissue ◽  
Aortic Smooth Muscle ◽  
Layer Technique ◽  
Layer By Layer Technique

Ca2+ signaling in fowl aortic smooth muscle increases during maturation but is impaired in neointimal plaques.

1998 ◽  
Vol 201 (11) ◽  
pp. 1695-1705 ◽  
Author(s):  
Z L Qin ◽  
H Nishimura
Keyword(s):  
Smooth Muscle ◽  
Arterial Pressure ◽  
Muscle Tissue ◽  
Sexual Maturation ◽  
Bird Species ◽  
Bay K 8644 ◽  
Dependent Manner ◽  
Smooth Muscle Tissue ◽  
Aortic Smooth Muscle ◽  
Dose Dependent

Many bird species show the spontaneous development of high arterial pressure and vascular lesions in the aorta and other large arteries. In chickens, arterial pressure tends to increase with age/maturation (particularly in males), and subendothelial hyperplasia (neointima) in the abdominal aorta is often seen prior to sexual maturation. The mechanisms involved, however, are not known. Our aim, therefore, was to determine (1) whether cytosolic Ca2+ signaling (CCS) responses to vasoactive substances in fowl aortic smooth muscle differ among chickens at different maturation stages and (2) whether CCS responses to Ca2+ channel agonists in neointimal plaques differ from those in normal aortic smooth muscle. K+ increased CCS in a dose-dependent manner in isolated and superfused abdominal aortic smooth muscle tissue from chicks (5-9 weeks old), pullets (11-18 weeks old) and adult hens (20 weeks and older); CCS responses increased as chickens matured. The addition of Bay K 8644 (10(-6)mol l-1) to Ringer's solution containing 50 mmol l-1 K+ further increased CCS, and this response was reduced to half by nifedipine (10(-6)mol l-1). Norepinephrine did not alter CCS in chicks, whereas marked dose-dependent increases in CCS were noted in pullets. In contrast to the CCS responses to K+, the norepinephrine-induced CCS responses became smaller in adult hens. Isolated neointimal plaques showed only slight increases in CCS in response to 50 mmol l-1 K+ plus Bay K 8644, whereas clear responses were noted in aortic smooth muscle tissue underlying the plaques. These results suggest (1) that CCS responses to Ca2+ channel agonists increased with sexual maturation in fowl, but (2) that CCS responses to norepinephrine were low in mature hens and to K+ plus Bay K 8644 were low in spontaneously developed neointima, suggesting that phenotypic modulation of Ca2+ channel/norepinephrine receptors may have occurred during maturation/aging and in neointima.

Pectin-Chitosan Multilayer Coated Microbeads of Diclofenac Sodium Prepared by Layer-by-Layer Technique

2014 ◽  
Vol 1060 ◽  
pp. 45-49
Author(s):  
Kamonrak Cheewatanakornkool ◽  
Pornsak Sriamornsak
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Diclofenac Sodium ◽  
Gastric Fluid ◽  
Layer By Layer ◽  
Release Behavior ◽  
Freeze Dried ◽  
Layer Technique ◽  
Calcium Pectinate ◽  
Layer By Layer Technique

The main objective of this study was to fabricate biopolymer-based microbeads, providing enteric properties and controlled release of diclofenac sodium, using layer-by-layer technique. The calcium pectinate microbeads have been designed and coated with chitosan and pectin multilayers. Drug release was performed in simulate gastric fluid (pH 1.2) for 2 hours, followed by pH 6.8 buffer for 8 hours. The effects of chitosan concentration, number of layer and drying technique on drug release were investigated. The results showed that the calcium pectinate microbeads could be simply prepared by ionotropic gelation and then coated with chitosan and pectin solutions using layer-by-layer procedure. The diameter of the microbeads ranged from 800 to 1000 μm for air-dried samples and from 1 to 2 mm for freeze-dried samples. The freeze-dried microbeads had a rough surface and many pores inside, as observed by SEM. The microbeads coated with 4% chitosan/4% pectin revealed a slower drug release than those coated with 1% chitosan/4% pectin and demonstrated a controlled release pattern. Moreover, different drying techniques and numbers of layer also influenced drug release behavior of the prepared microbeads.

A collagen matrix derived from bladder can be used to engineer smooth muscle tissue

2008 ◽  
Vol 26 (4) ◽  
pp. 307-314 ◽  
Author(s):  
Byung-Soo Kim ◽  
Anthony Atala ◽  
James J. Yoo
Keyword(s):  
Smooth Muscle ◽  
Muscle Tissue ◽  
Collagen Matrix ◽  
Smooth Muscle Tissue

Highly transparent and self-healing films based on the dynamic Schiff base linkage

RSC Advances ◽  
2016 ◽  
Vol 6 (116) ◽  
pp. 115247-115251 ◽  
Author(s):  
Jiaoyu Ren ◽  
Yanxi Zhu ◽  
Hongyun Xuan ◽  
Xuefan Liu ◽  
Zhichao Lou ◽  
...  
Keyword(s):  
Schiff Base ◽  
Layer By Layer ◽  
Self Healing ◽  
Layer Technique ◽  
Layer By Layer Technique

In this study, we report highly transparent self-healing films based on Schiff base linkages between DF-PEG and CS via a layer-by-layer technique.

Sign in / Sign up

Export Citation Format

Share Document