Angiogenesis and osteogenesis enhanced by bFGF ex vivo gene therapy for bone tissue engineering in reconstruction of calvarial defects

2011 ◽  
Vol 96A (3) ◽  
pp. 543-551 ◽  
Author(s):  
Dan Qu ◽  
Jihua Li ◽  
Yubao Li ◽  
Ying Gao ◽  
Yi Zuo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Ex Vivo ◽  
Calvarial Defects ◽  
Ex Vivo Gene Therapy

scholarly journals Vascularization Strategies in Bone Tissue Engineering

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1749
Author(s):  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Critical Size ◽  
Ex Vivo ◽  
Angiogenic Growth Factors ◽  
Hypoxic Conditions ◽  
Surgical Methods ◽  
Artificial Tissue

Bone is a highly vascularized tissue, and its development, maturation, remodeling, and regeneration are dependent on a tight regulation of blood vessel supply. This condition also has to be taken into consideration in the context of the development of artificial tissue substitutes. In classic tissue engineering, bone-forming cells such as primary osteoblasts or mesenchymal stem cells are introduced into suitable scaffolds and implanted in order to treat critical-size bone defects. However, such tissue substitutes are initially avascular. Because of the occurrence of hypoxic conditions, especially in larger tissue substitutes, this leads to the death of the implanted cells. Therefore, it is necessary to devise vascularization strategies aiming at fast and efficient vascularization of implanted artificial tissues. In this review article, we present and discuss the current vascularization strategies in bone tissue engineering. These are based on the use of angiogenic growth factors, the co-implantation of blood vessel forming cells, the ex vivo microfabrication of blood vessels by means of bioprinting, and surgical methods for creating surgically transferable composite tissues.

Comparison among Four Kinds of Bone Tissue Engineering Scaffold

2007 ◽  
Vol 330-332 ◽  
pp. 963-966 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Cai Li ◽  
Wei Dong Tian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Autogenous Bone ◽  
Calvarial Defects ◽  
Reconstruction Of Bone Defects

Bone tissue engineering is a promising way to repair of bone defects. To choose a proper scaffold is still a disputable problem in bone tissue engineering. This study aimed to compare the effects of repairing critical calvarial defects with the compounds of autogenous bone marrow stromal cells (BMSCs) and coral hydroxyapatite(CHA), hydroxyapatite/ tricalcium phosphate (HA/TCP), poly(lactide-co-glycolide) (PLGA) and alginate (AG). The results showed that CHA and AG were satisfactory bone tissues engineering scaffolds among the four kinds of materials. BMSCs/CHA and BMSCs/AG are promising techniques for reconstruction of bone defects.

scholarly journals Non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 29 (2) ◽  
pp. 206-220 ◽  
Author(s):  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J.A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy

scholarly journals Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

2020 ◽  
Vol 11 ◽  
pp. 204173142094273
Author(s):  
Karen M Marshall ◽  
Janos M Kanczler ◽  
Richard OC Oreffo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Experimental Methodology ◽  
Chick Chorioallantoic Membrane ◽  
Recent Developments ◽  
Chorioallantoic Membrane Assay

The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

scholarly journals Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Sahar Ansari ◽  
Marcelo O. Freire ◽  
Eun-Kyoung Pang ◽  
Alaa I. Abdelhamid ◽  
Mohammad Almohaimeed ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
De Novo ◽  
Isotype Control ◽  
Different Types ◽  
Calvarial Defects

Biomaterials are widely used as scaffolds for tissue engineering. We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR). The purpose of the current study was to compare the efficacy of immobilization of a specific murine anti-BMP-2 mAb on three different types of biomaterials and to evaluate their suitability as scaffolds for AMOR. Anti-BMP-2 mAb or isotype control mAb was immobilized on titanium (Ti) microbeads, alginate hydrogel, and ACS. The treated biomaterials were surgically implanted in rat critical-sized calvarial defects. After 8 weeks,de novobone formation was assessed using micro-CT and histomorphometric analyses. Results showedde novobone regeneration with all three scaffolds with immobilized anti-BMP-2 mAb, but not isotype control mAb. Ti microbeads showed the highest volume of bone regeneration, followed by ACS. Alginate showed the lowest volume of bone. Localization of BMP-2, -4, and -7 antigens was detected on all 3 scaffolds with immobilized anti-BMP-2 mAb implanted in calvarial defects. Altogether, these data suggested a potential mechanism for bone regeneration through entrapment of endogenous BMP-2, -4, and -7 proteins leading to bone formation using different types of scaffoldsviaAMOR.

scholarly journals Gene therapy for bone tissue engineering

2016 ◽  
Vol 13 (2) ◽  
pp. 111-125 ◽  
Author(s):  
Young-Dong Kim ◽  
Prasad Pofali ◽  
Tae-Eun Park ◽  
Bijay Singh ◽  
Kihyun Cho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Porous bioprinted constructs in BMP-2 non-viral gene therapy for bone tissue engineering

2013 ◽  
Vol 1 (48) ◽  
pp. 6619 ◽  
Author(s):  
Loek D. Loozen ◽  
Fiona Wegman ◽  
F. Cumhur Öner ◽  
Wouter J. A. Dhert ◽  
Jacqueline Alblas
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Viral Gene ◽  
Viral Gene Therapy
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