Biochemical comparisons of osteoarthritic human synovial fluid with calf sera used in knee simulator wear testing

2010 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
J.-M. Brandt ◽  
L. K. Brière ◽  
J. Marr ◽  
S. J. MacDonald ◽  
R. B. Bourne ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Wear Testing ◽  
Knee Simulator ◽  
Human Synovial Fluid

scholarly journals Isolation of human synovial-fluid hyaluronate by density-gradient ultracentrifugation and evaluation of its protein content

1972 ◽  
Vol 126 (5) ◽  
pp. 1073-1080 ◽  
Author(s):  
Irwin Scher ◽  
David Hamerman
Keyword(s):  
Synovial Fluid ◽  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Proteolytic Digestion ◽  
Peptide Fragments ◽  
Guanidinium Chloride ◽  
Caesium Chloride ◽  
Normal Human ◽  
Protein Moiety ◽  
Human Synovial Fluid

1. A compound of hyaluronate and protein, called hyaluronate–protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate–protein was labelled with [125I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety (125I counts) and hyaluronate (hexuronate) sedimented together in the middle of the gradient. (b) The labelled hyaluronate–protein was treated with trypsin, and ultracentrifugation showed that peptide fragments (125I counts) were dispersed throughout the gradient, indicating proteolytic digestion. Hyaluronate sedimented in the middle of the gradient. (c) The labelled hyaluronate–protein was digested with streptococcal hyaluronidase, and ultracentrifugation showed that hyaluronate fragments were dispersed throughout the gradient, indicating digestion of the polysaccharide. The protein moiety, without attached hyaluronate, now sedimented at the top of the gradient. (d) Ultracentrifugation of labelled hyaluronate–protein in 4m-guanidinium chloride showed that protein and hyaluronate sedimented together. 3. These studies confirm that hyaluronate is combined with a small quantity of protein in normal human synovial fluid. A mild method for the rapid isolation of hyaluronate–protein in good yield is described.

scholarly journals The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells

2017 ◽  
Vol 487 (2) ◽  
pp. 457-463 ◽  
Author(s):  
Farong Ou ◽  
Kai Su ◽  
Jiadong Sun ◽  
Wenting Liao ◽  
Yu Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Fluid ◽  
Human Synovial Fluid

scholarly journals SAT0028 The importance of proper handling of human synovial fluid for arthritis research

2018 ◽  
Author(s):  
H. Brouwers ◽  
J.H. von Hegedus ◽  
R.E.M. Toes ◽  
T.W.J. Huizinga ◽  
M.A. Giera ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Human Synovial Fluid

scholarly journals Effect of human synovial fluid in models of in vitro chondrogenic re-differentiation of human primary chondrocytes

2016 ◽  
Vol 24 ◽  
pp. S166
Author(s):  
C. Galeano-Garces ◽  
S.M. Riester ◽  
E.T. Camilleri ◽  
H.S. Ryan ◽  
J. Smith ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Primary Chondrocytes ◽  
Human Primary Chondrocytes ◽  
Human Synovial Fluid

Three-dimensional origami paper-based device for portable immunoassay applications

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 598-607 ◽  
Author(s):  
Chung-An Chen ◽  
Wen-Shin Yeh ◽  
Tsung-Ting Tsai ◽  
Yu-De Li ◽  
Chien-Fu Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Synovial Fluid ◽  
Three Dimensional ◽  
Surface Modified ◽  
Human Synovial Fluid ◽  
Dimensional Surface ◽  
Analytical Device

A three-dimensional surface-modified origami-paper-based analytical device for Staphylococcus aureus detection in highly viscous human synovial fluid.

scholarly journals Immunologic studies of the proteins of human synovial fluid

1963 ◽  
Vol 6 (2) ◽  
pp. 115-129 ◽  
Author(s):  
Peter H. Schur ◽  
John Sandson
Keyword(s):  
Synovial Fluid ◽  
Human Synovial Fluid

scholarly journals Quantification of peptides in human synovial fluid using liquid chromatography–tandem mass spectrometry

Talanta ◽  
2018 ◽  
Vol 186 ◽  
pp. 124-132 ◽  
Author(s):  
Araceli Garcia-Ac ◽  
Sung Vo Duy ◽  
Sébastien Sauvé ◽  
Florina Moldovan ◽  
V. Gaëlle Roullin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Synovial Fluid ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Human Synovial Fluid

Wear Testing of UHMWPE Tibial Components: Influence of Oxidation

1999 ◽  
Vol 122 (1) ◽  
pp. 323-331 ◽  
Author(s):  
Sarah K. Young ◽  
Tony S. Keller ◽  
Keith W. Greer ◽  
Michael C. Gorhan
Keyword(s):  
Gamma Irradiation ◽  
Wear Testing ◽  
Low Level ◽  
Valid Method ◽  
Knee Simulator ◽  
High Oxidation

An AMTI knee simulator was used to evaluate the wear of UHMWPE tibial inserts which were gamma sterilized in air and displayed a high or low level of oxidation. After 5 million cycles, four out of five samples from the high oxidation group displayed subsurface cracking and/or delamination. The five specimens in the low oxidation group experienced burnishing only. These results indicate that gamma irradiation in air together with high oxidation due to shelf aging can increase susceptibility to wear. The appearance of the wear scars indicates that knee simulator testing is a valid method of producing burnishing, cracking, and delamination similar to that seen in-vivo. [S0742-4787(00)04301-0]

Sign in / Sign up

Export Citation Format

Share Document