scholarly journals Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells

2010 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Eric Hesse ◽  
Theresa E. Hefferan ◽  
James E. Tarara ◽  
Carl Haasper ◽  
Rupert Meller ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Collagen Type ◽  
Collagen Type I ◽  
Marrow Stromal Cells ◽  
Type I ◽  
Rat Bone

scholarly journals Pro-fibrotic phenotype of bone marrow stromal cells in Modic type 1 changes

2021 ◽  
Vol 41 ◽  
pp. 648-667
Author(s):  
I Heggli ◽  
◽  
S Epprecht ◽  
A Juengel ◽  
R Schuepbach ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Collagen Type ◽  
Collagen Type I ◽  
Marrow Stromal Cells ◽  
Type I ◽  
Cell Contractility ◽  
Modic Type

Modic type 1 changes (MC1) are painful vertebral bone marrow lesions frequently found in patients suffering from chronic low-back pain. Marrow fibrosis is a hallmark of MC1. Bone marrow stromal cells (BMSCs) are key players in other fibrotic bone marrow pathologies, yet their role in MC1 is unknown. The present study aimed to characterise MC1 BMSCs and hypothesised a pro-fibrotic role of BMSCs in MC1. BMSCs were isolated from patients undergoing lumbar spinal fusion from MC1 and adjacent control vertebrae. Frequency of colony-forming unit fibroblast (CFU-F), expression of stem cell surface markers, differentiation capacity, transcriptome, matrix adhesion, cell contractility as well as expression of pro-collagen type I alpha 1, α-smooth muscle actin, integrins and focal adhesion kinase (FAK) were compared. More CFU-F and increased expression of C-X-C-motif-chemokine 12 were found in MC1 BMSCs, possibly indicating overrepresentation of a perisinusoidal BMSC population. RNA sequencing analysis showed enrichment in extracellular matrix proteins and fibrosis-related signalling genes. Increases in pro-collagen type I alpha 1 expression, cell adhesion, cell contractility and phosphorylation of FAK provided further evidence for their pro-fibrotic phenotype. Moreover, a leptin receptor high expressing (LEPRhigh) BMSC population was identified that differentiated under transforming growth factor beta 1 stimulation into myofibroblasts in MC1 but not in control BMSCs. In conclusion, pro-fibrotic changes in MC1 BMSCs and a LEPRhigh MC1 BMSC subpopulation susceptible to myofibroblast differentiation were found. Fibrosis is a hallmark of MC1 and a potential therapeutic target. A causal link between the pro-fibrotic phenotype and clinical characteristics needs to be demonstrated.

Repair of large cranial defects byhBMP-2expressing bone marrow stromal cells: Comparison between alginate and collagen type I systems

2010 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Sophia Chia Ning Chang ◽  
Hui-Ying Chung ◽  
Ching-Lung Tai ◽  
Philips Kuo Ting Chen ◽  
Tsung-Min Lin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Collagen Type ◽  
Collagen Type I ◽  
Marrow Stromal Cells ◽  
Type I

Solanum Muricatum Promotes Osteogenic Differentiation of Rat Bone Marrow Stromal Cells

2017 ◽  
Vol 82 (7) ◽  
pp. 1775-1780 ◽  
Author(s):  
Nan Wang ◽  
Feng Wang ◽  
Youshui Gao ◽  
Zubin Zhou ◽  
Wei Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Solanum Muricatum ◽  
Rat Bone

scholarly journals Regulation of osteogenic differentiation of rat bone marrow stromal cells on 2D nanorod substrates

Biomaterials ◽  
2010 ◽  
Vol 31 (7) ◽  
pp. 1732-1741 ◽  
Author(s):  
Gagandeep Kaur ◽  
Mani T. Valarmathi ◽  
Jay D. Potts ◽  
Esmaiel Jabbari ◽  
Tara Sabo-Attwood ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Rat Bone
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