Generation of reactive oxygen species from porous silicon microparticles in cell culture medium

2009 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Suet Peng Low ◽  
Keryn A. Williams ◽  
Leigh T. Canham ◽  
Nicolas H. Voelcker
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Culture ◽  
Porous Silicon ◽  
Culture Medium ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cell Culture Medium

scholarly journals Cellular Effects of T-2 Toxin on Primary Hepatic Cell Culture Models of Chickens

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 46 ◽  
Author(s):  
Máté Mackei ◽  
Kata Orbán ◽  
Andor Molnár ◽  
László Pál ◽  
Károly Dublecz ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Culture ◽  
Cell Cultures ◽  
Metabolic Activity ◽  
Incubation Time ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cellular Effects ◽  
Culture Models ◽  
Cell Culture Models

Trichothecene mycotoxins such as T-2 toxin cause severe problems for agriculture, as well as for veterinary medicine. As liver is one of the key organs in metabolism, the main aim of our study was to investigate the immunomodulatory and cytotoxic effects of T-2 toxin, using primary hepatocyte mono-culture and hepatocyte—nonparenchymal cell (predominantly Kupffer cell) co-culture models of chicken. Cultures were exposed to 10 (T10 group), 100 (T100 group) and 1000 (T1000 group) nmol/L T-2 toxin treatment for 8 or 24 h. Alterations of cellular metabolic activity, the production of reactive oxygen species (extracellular H2O2), heat shock protein 70 (HSP70), and the concentration of different inflammatory cytokines such as interleukin (IL-)6 and IL-8 were investigated. Metabolic activity was intensely decreased by T-2 toxin administration in all of the cell culture models, in every applied concentration and incubation time. Concentrations of HSP70 and IL-8 were significantly increased in hepatocyte mono-cultures exposed to higher T-2 toxin levels (both in T100 and T1000 groups for HSP70 and in T1000 group for IL-8, respectively) compared to controls after 24 h incubation. Similarly, IL-6 levels were also significantly elevated in the T100 and T1000 groups in both of mono- and co-cultures, but only after 8 h of incubation time. In spite of the general harmful effects of T-2 toxin treatment, no significant differences were observed on reactive oxygen species production. Furthermore, the two cell culture models showed different levels of H2O2, HSP70, and IL-8 concentrations independently of T-2 toxin supplementation. In conclusion, the established primary cell cultures derived from chicken proved to be proper models to study the specific molecular effects caused by T-2 toxin. Metabolic activity and immune status of the different examined cell cultures were intensively affected; however, no changes were found in H2O2 levels.

scholarly journals Cell-biological effects of zinc oxide spheres and rods from the nano- to the microscale at sub-toxic levels

2020 ◽  
Author(s):  
M. Olejnik ◽  
M. Kersting ◽  
N. Rosenkranz ◽  
K. Loza ◽  
M. Breisch ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Zinc Oxide ◽  
Zinc Acetate ◽  
Culture Medium ◽  
Biological Effects ◽  
Oxygen Species ◽  
Cell Culture Medium ◽  
Oxide Particles ◽  
Zinc Oxide Particles

AbstractZinc oxide particles were synthesized in various sizes and shapes, i.e., spheres of 40-nm, 200-nm, and 500-nm diameter and rods of 40∙100 nm2 and 100∙400 nm2 (all PVP-stabilized and well dispersed in water and cell culture medium). Crystallographically, the particles consisted of the hexagonal wurtzite phase with a primary crystallite size of 20 to 100 nm. The particles showed a slow dissolution in water and cell culture medium (both neutral; about 10% after 5 days) but dissolved within about 1 h in two different simulated lysosomal media (pH 4.5 to 4.8). Cells relevant for respiratory exposure (NR8383 rat alveolar macrophages) were exposed to these particles in vitro. Viability, apoptosis, and cell activation (generation of reactive oxygen species, ROS, release of cytokines) were investigated in an in vitro lung cell model with respect to the migration of inflammatory cells. All particle types were rapidly taken up by the cells, leading to an increased intracellular zinc ion concentration. The nanoparticles were more cytotoxic than the microparticles and comparable with dissolved zinc acetate. All particles induced cell apoptosis, unlike dissolved zinc acetate, indicating a particle-related mechanism. Microparticles induced a stronger formation of reactive oxygen species than smaller particles probably due to higher sedimentation (cell-to-particle contact) of microparticles in contrast to nanoparticles. The effect of particle types on the cytokine release was weak and mainly resulted in a decrease as shown by a protein microarray. In the particle-induced cell migration assay (PICMA), all particles had a lower effect than dissolved zinc acetate. In conclusion, the biological effects of zinc oxide particles in the sub-toxic range are caused by zinc ions after intracellular dissolution, by cell-to-particle contacts, and by the uptake of zinc oxide particles into cells.

Hydrocyanines: a versatile family of probes for imaging radical oxidants in vitro and in vivo

2017 ◽  
Vol 2 (3) ◽  
pp. 191-200 ◽  
Author(s):  
Corinne M. Sadlowski ◽  
Santanu Maity ◽  
Kousik Kundu ◽  
Niren Murthy
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Culture ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Tissue Explants

The hydrocyanines are a class of dyes that can detect reactive oxygen species (ROS) in cell culture, tissue explants, and in vivo.

Light-dependent generation of reactive oxygen species in cell culture media

2001 ◽  
Vol 30 (12) ◽  
pp. 1418-1425 ◽  
Author(s):  
Agnieszka Grzelak ◽  
Błażej Rychlik ◽  
Grzegorz Bartosz
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Culture ◽  
Culture Media ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cell Culture Media

84 PTEROSTILBENE CAN REDUCE THE PERCENTAGE OF LIPIDS AND REACTIVE OXYGEN SPECIES IN IN VITRO-PRODUCED BOVINE EMBRYOS

2017 ◽  
Vol 29 (1) ◽  
pp. 149
Author(s):  
F. Sosa ◽  
J. Fernando de la Torre ◽  
H. Álvarez ◽  
S. Pérez ◽  
M. E. Kjelland ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Culture Medium ◽  
Statistical Significance ◽  
Cell Mass ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Embryo Production ◽  
Cell Counts ◽  
Significant Difference ◽  
And Control

The actual challenge for the majority of research centers involves the embryo culture medium, since it is known that the culture medium plays a large role in determining embryo quality. Pterostilbene (PT) is a natural analogue of Resveratrol, an antioxidant that can reduce lipids in embryos, but no reports exist of PT being used with IVF-produced embryos or gametes. The objective of the present research was to evaluate the effect of PT in culture media CDM1 and CDM2 on embryo production, cell count, lipid accumulation, and reactive oxygen species (ROS). A total of 4 concentrations of PT and a control were evaluated, i.e. 3, 1, 0.33, 0.11, and 0 μM, in 2 separate experiments. The first experiment was performed using 6 replicates (n = 204) to evaluate blastocyst production (n = 201) and determine the percentage of lipids using the stain Sudan-Black, (n = 100). Hoechst 33258 and propidium iodide were used for determining cell counts. The second experiment was performed using 7 replicates, the effect of using PT (0.33 μM) was compared with a control with 2 O2 concentrations (5 and 20%) for evaluating ROS production (n = 124). Blastocysts without zona pellucida were incubated 48 h at 38.5°C in PBS (without polyvinyl alcohol) with 60 μL of ro-green fluorescent protein. After incubation, 25 μL of 4′,6-diamidino-2-phenylindole (1 mg mL−1) was added and incubated for 5 min. A fluorescence microscope was used and positive ROS particles digitized using Photoshop CS6 and quantified using the program ImageJ®. The data were transformed to arcsin values for subsequent analysis. In the first experiment, ANOVA and least significant difference tests were used to determine statistical significance (Statgraphics). No significant differences were found among treatments (P > 0.05) for the internal cell mass: 3, 1, 0.33, 0.11 μM PT (29.2 ± 5.2; 28.9 ± 3.8; 22.2 ± 3.2; 29.0 ± 1.7, respectively) and the control (27.0 ± 1.7). The cells of the trophectoderm did not differ (P > 0.05) between treatments (31.7 ± 4.8; 31.3 ± 3.9; 38.6 ± 3.5; 30.8 ± 1.8) and control (33.4 ± 2.1). Total cells did not differ (P > 0.05) between treatments (72.0 ± 9.8; 82.6 ± 4.3; 94.8 ± 12.8; 73.2 ± 9.2) and control (83.8 ± 7.7). Embryo production (Day 8) was greater for control (33.5 ± 3.0) versus treatments (14.1 ± 1.7; 19.4 ± 1.9; 21.1 ± 2.6; 20.8 ± 2.1) (P < 0.05); however, PT reduced the percentage of lipids (11.0 ± 0.8; 10.7 ± 0.9; 11.6 ± 1.3; 11.3 ± 1.1) within the cytoplasm of the embryos (P < 0.05) versus control (17.01 ± 1.20). In the second experiment, a factorial 2 × 2 matrix demonstrated that the O2 concentration did not have an effect on ROS (P > 0.05); however, the PT had a significant effect on the reduction of ROS (P < 0.05), i.e. a negative correlation, r = −0.835. In summary, we determined that PT did not improve the production of blastocysts but resulted in a significant reduction of ROS and lipids.

Cell culture system for the controlled intracellular generation of reactive oxygen species

1997 ◽  
Vol 11 (5) ◽  
pp. 569-572 ◽  
Author(s):  
H. Hermersdörfer ◽  
B. Ozierenski ◽  
W.A. Schmalix ◽  
J. Doehmer ◽  
H.R. Glatt
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Culture ◽  
Culture System ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cell Culture System

Production of Reactive Oxygen and Nitrogen Species in a Cell Culture Medium Exposed to an Atmospheric Pressure Plasma Jet

MRS Advances ◽  
2017 ◽  
Vol 2 (18) ◽  
pp. 987-993 ◽  
Author(s):  
Hirofumi Kurita ◽  
Junichiro Miyamoto ◽  
Saki Miyachika ◽  
Yoshito Uchihashi ◽  
Hachiro Yasuda ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Liquid Surface ◽  
Reactive Oxygen ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Cell Culture Medium ◽  
Nitrogen Species ◽  
Pressure Plasma

ABSTRACTCold atmospheric pressure plasma has been intensively studied due to growing interest in biomedical applications. For example, it has been revealed that the plasma-irradiated cell culture medium (PAM: Plasma Activated Medium) shows antitumor effect as well as the direct plasma treatment. Long-lived reactive oxygen and nitrogen species (RONS), such as hydrogen peroxide (H2O2), nitrite (NO2-), and nitrate (NO3-), are considered as a main product in PAM. Therefore, investigation of the production characteristics of RONS in PAM with different experimental parameters is important. Here, we focused on humidification of feed gas (argon or helium) and effect of gap between the nozzle of the plasma jet and liquid surface. As a result, H2O2 concentration was increased by humidification of both feed gases. However, the concentration of NO2- and NO3- was decreased by the humidification of helium. In addition, the remarkable effect of the humidification on H2O2 production was observed when the plasma jet was in contact with the liquid surface.

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