Polysaccharide-based vaccine delivery systems: Macromolecular assembly, interactions with antigen presenting cells, andin vivoimmunomonitoring

2009 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Caroline Weber ◽  
Alexandre Drogoz ◽  
Laurent David ◽  
Alain Domard ◽  
Marie-Hélène Charles ◽  
...  
Keyword(s):  
Vaccine Delivery ◽  
Antigen Presenting Cells ◽  
Delivery Systems ◽  
Macromolecular Assembly ◽  
Antigen Presenting

Cellular uptake of polylactide particles induces size dependent cytoskeletal remodeling in antigen presenting cells

2021 ◽  
Author(s):  
Jairam Meena ◽  
Dinesh Giri Goswami ◽  
Chakkumkal Anish ◽  
Amulya K. Panda
Keyword(s):  
Dendritic Cells ◽  
Cellular Uptake ◽  
Vaccine Delivery ◽  
Antigen Presenting Cells ◽  
Delivery Systems ◽  
Immune Mechanism ◽  
Size Dependent ◽  
Cytoskeletal Remodeling ◽  
Antigen Capture ◽  
Antigen Presenting

Phagocytosis of particulate vaccine delivery systems is a critical immune mechanism involved in antigen capture and processing by macrophages and dendritic cells. The internalization and degradation of the particles involves...

scholarly journals Effective Activation of Human Antigen-Presenting Cells and Cytotoxic CD8+ T Cells by a Calcium Phosphate-Based Nanoparticle Vaccine Delivery System

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 110 ◽  
Author(s):  
Florian Scheffel ◽  
Torben Knuschke ◽  
Lucas Otto ◽  
Sebastian Kollenda ◽  
Viktoriya Sokolova ◽  
...  
Keyword(s):  
T Cells ◽  
Calcium Phosphate ◽  
Delivery System ◽  
Cd8 T Cells ◽  
Vaccine Delivery ◽  
Antigen Presenting Cells ◽  
Poly I:C ◽  
Human Virus ◽  
Vaccine Delivery System ◽  
Antigen Presenting

The ability of vaccines to induce T cell responses is crucial for preventing diseases caused by viruses. Nanoparticles (NPs) are considered to be efficient tools for the initiation of potent immune responses. Calcium phosphate (CaP) NPs are a class of biodegradable nanocarriers that are able to deliver immune activating molecules across physiological barriers. Therefore, the aim of this study was to assess whether Toll-like receptor (TLR) ligand and viral antigen functionalized CaP NPs are capable of inducing efficient maturation of human antigen presenting cells (APC). To achieve this, we generated primary human dendritic cells (DCs) and stimulated them with CpG or poly(I:C) functionalized CaP NPs. DCs were profoundly stronger when activated upon NP stimulation compared to treatment with soluble TLR ligands. This is indicated by increased levels of costimulatory molecules and the secretion of proinflammatory cytokines. Consequently, coculture of NP-stimulated APCs with CD8+ T cells resulted in a significant expansion of virus-specific T cells. In summary, our data suggest that functionalized CaP NPs are a suitable tool for activating human virus-specific CD8+ T cells and may represent an excellent vaccine delivery system.

scholarly journals Cell-penetrating Peptides: Efficient Vectors for Vaccine Delivery

2019 ◽  
Vol 16 (5) ◽  
pp. 430-443 ◽  
Author(s):  
Jieru Yang ◽  
Yacheng Luo ◽  
Mohini Anjna Shibu ◽  
Istvan Toth ◽  
Mariusz Skwarczynskia
Keyword(s):  
Drug Transport ◽  
Vaccine Delivery ◽  
Cell Penetrating Peptides ◽  
Delivery Systems ◽  
Antigen Delivery ◽  
Antigen Uptake ◽  
Cell Penetrating ◽  
The Stability ◽  
Antigen Presenting

Subunit vaccines are composed of pathogen fragments that, on their own, are generally poorly immunogenic. Therefore, the incorporation of an immunostimulating agent, e.g. adjuvant, into vaccine formulation is required. However, there are only a limited number of licenced adjuvants and their immunostimulating ability is often limited, while their toxicity can be substantial. To overcome these problems, a variety of vaccine delivery systems have been proposed. Most of them are designed to improve the stability of antigen in vivo and its delivery into immune cells. Cell-penetrating peptides (CPPs) are especially attractive component of antigen delivery systems as they have been widely used to enhance drug transport into the cells. Fusing or co-delivery of antigen with CPPs can enhance antigen uptake, processing and presentation by antigen presenting cells (APCs), which are the fundamental steps in initiating an immune response. This review describes the different mechanisms of CPP intercellular uptake and various CPP-based vaccine delivery strategies.

scholarly journals Dual stimulation of antigen presenting cells using carbon nanotube-based vaccine delivery system for cancer immunotherapy

Biomaterials ◽  
2016 ◽  
Vol 104 ◽  
pp. 310-322 ◽  
Author(s):  
Hatem A.F.M. Hassan ◽  
Lesley Smyth ◽  
Julie T.-W. Wang ◽  
Pedro M. Costa ◽  
Kulachelvy Ratnasothy ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Cancer Immunotherapy ◽  
Delivery System ◽  
Vaccine Delivery ◽  
Antigen Presenting Cells ◽  
Vaccine Delivery System ◽  
Antigen Presenting ◽  
Stimulation Of

scholarly journals An Overview on the Field of Micro- and Nanotechnologies for Synthetic Peptide-Based Vaccines

2011 ◽  
Vol 2011 ◽  
pp. 1-18 ◽  
Author(s):  
Aiala Salvador ◽  
Manoli Igartua ◽  
Rosa Maria Hernández ◽  
José Luis Pedraz
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Innate Immune System ◽  
Synthetic Peptide ◽  
Vaccine Delivery ◽  
Delivery Systems ◽  
Innate Immune ◽  
Cross Presentation ◽  
Antigen Presenting

The development of synthetic peptide-based vaccines has many advantages in comparison with vaccines based on live attenuated organisms, inactivated or killed organism, or toxins. Peptide-based vaccines cannot revert to a virulent form, allow a better conservation, and are produced more easily and safely. However, they generate a weaker immune response than other vaccines, and the inclusion of adjuvants and/or the use of vaccine delivery systems is almost always needed. Among vaccine delivery systems, micro- and nanoparticulated ones are attractive, because their particulate nature can increase cross-presentation of the peptide. In addition, they can be passively or actively targeted to antigen presenting cells. Furthermore, particulate adjuvants are able to directly activate innate immune system in vivo. Here, we summarize micro- and nanoparticulated vaccine delivery systems used in the field of synthetic peptide-based vaccines as well as strategies to increase their immunogenicity.

scholarly journals Carbohydrate Immune Adjuvants in Subunit Vaccines

Pharmaceutics ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 965
Author(s):  
Sahra Bashiri ◽  
Prashamsa Koirala ◽  
Istvan Toth ◽  
Mariusz Skwarczynski
Keyword(s):  
Immune Responses ◽  
Delivery System ◽  
Antigen Presenting Cells ◽  
Delivery Systems ◽  
Subunit Vaccines ◽  
Immune Adjuvants ◽  
Carbohydrate Ligands ◽  
Antigen Presenting ◽  
Molecular Patterns

Modern subunit vaccines are composed of antigens and a delivery system and/or adjuvant (immune stimulator) that triggers the desired immune responses. Adjuvants mimic pathogen-associated molecular patterns (PAMPs) that are typically associated with infections. Carbohydrates displayed on the surface of pathogens are often recognized as PAMPs by receptors on antigen-presenting cells (APCs). Consequently, carbohydrates and their analogues have been used as adjuvants and delivery systems to promote antigen transport to APCs. Carbohydrates are biocompatible, usually nontoxic, biodegradable, and some are mucoadhesive. As such, carbohydrates and their derivatives have been intensively explored for the development of new adjuvants. This review assesses the immunological functions of carbohydrate ligands and their ability to enhance systemic and mucosal immune responses against co-administered antigens. The role of carbohydrate-based adjuvants/delivery systems in the development of subunit vaccines is discussed in detail.

Antigen-presenting cells and tolerance induction

Allergy ◽  
2002 ◽  
Vol 57 (1) ◽  
pp. 2-8 ◽  
Author(s):  
D. von Bubnoff ◽  
H. de la Salle ◽  
J. Wessendorf ◽  
S. Koch ◽  
D. Hanau ◽  
...  
Keyword(s):  
Tolerance Induction ◽  
Antigen Presenting Cells ◽  
Antigen Presenting
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