Mixing conditions for cell scaffolds affect the bone formation induced by bone engineering with human bone marrow stromal cells, β-tricalcium phosphate granules, and rhBMP-2

2009 ◽  
Vol 91A (1) ◽  
pp. 84-91 ◽  
Author(s):  
Moritoshi Uchida ◽  
Hideki Agata ◽  
Hiroshi Sagara ◽  
Yoshinori Shinohara ◽  
Hideaki Kagami ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Mixing Conditions ◽  
Cell Scaffolds

Bone Formation in Transplants of Human Bone Marrow Stromal Cells and Hydroxyapatite–Tricalcium Phosphate: Prediction with Quantitative CT in Mice

Radiology ◽  
2004 ◽  
Vol 230 (2) ◽  
pp. 369-376 ◽  
Author(s):  
Mahesh H. Mankani ◽  
Sergei A. Kuznetsov ◽  
Nilo A. Avila ◽  
Albert Kingman ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Quantitative Ct

scholarly journals Lamellar Spacing in Cuboid Hydroxyapatite Scaffolds Regulates Bone Formation by Human Bone Marrow Stromal Cells

2011 ◽  
Vol 17 (11-12) ◽  
pp. 1615-1623 ◽  
Author(s):  
Mahesh H. Mankani ◽  
Shahrzad Afghani ◽  
Jaime Franco ◽  
Max Launey ◽  
Sally Marshall ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Lamellar Spacing ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Effects of dexamethasone on proliferation, activity, and cytokine secretion of normal human bone marrow stromal cells: possible mechanisms of glucocorticoid-induced bone loss

1999 ◽  
Vol 162 (3) ◽  
pp. 371-379 ◽  
Author(s):  
CH Kim ◽  
SL Cheng ◽  
GS Kim
Keyword(s):  
Bone Marrow ◽  
Bone Resorption ◽  
Bone Loss ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Cytokine Secretion ◽  
Human Bone Marrow

It is well documented that glucocorticoid excess causes bone loss, but the mechanisms of these effects remain poorly defined. To understand further the mechanisms of glucocorticoid-induced osteoporosis, we investigated the effects of glucocorticoids on bone formation and bone resorption by examining the proliferation, functional activities, and cytokine secretion of cultured human bone marrow stromal cells (hBMSC). Treatment with dexamethasone for 24 h at the concentration of 10(-8) M significantly suppressed [(3)H]thymidine incorporation and further inhibition was observed with longer treatment (8 days) or higher concentration (10(-7) M). Alkaline phosphatase activity of hBMSC was markedly stimulated with addition of dexamethasone (10(-8) M), to 191 +/- 22% (after 4 days) and 317 +/- 46% (after 7 days) of control. Dexamethasone (10(-8) M) treatment for 48 h decreased the incorporation of [(3)H]proline into collagenase-digestible protein (CDP; 43.7+/-7.9% of control) and non-collagen protein (65.2+/-8.4% of control), with a greater effect on CDP. Northern blot analysis indicated that alpha1(I)-collagen mRNA level was decreased by dexamethasone to 27.6 +/- 9.0% of the control value after 1 day of exposure, and to 55.2 +/- 6.2% after 7 days. Dexamethasone markedly suppressed basal production of interleukin (IL)-6 and IL-11 and that stimulated by parathyroid hormone (PTH), IL-1alpha, or tumour necrosis factor-alpha in a dose-dependent manner. These results suggest that the glucocorticoid-induced bone loss is derived at least in part via inhibition of bone formation, which includes the suppression of osteoblast proliferation and collagen synthesis. As both basal and PTH-stimulated production of IL-6 and IL-11 are decreased by dexamethasone, the increased bone resorption observed in glucocorticoid-induced osteopenia does not appear to be mediated by IL-6 or IL-11.

scholarly journals Incorporation of Fucoidan in β-Tricalcium phosphate-Chitosan scaffold prompts the differentiation of human bone marrow stromal cells into osteogenic lineage

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Subramaniam Puvaneswary ◽  
Hanumantharao Balaji Raghavendran ◽  
Sepehr Talebian ◽  
Malliga Raman Murali ◽  
Suhaeb A Mahmod ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Tricalcium Phosphate ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Chitosan Scaffold ◽  
Osteogenic Lineage

In Vivo Bone Formation by Human Bone Marrow Stromal Cells: Reconstruction of the Mouse Calvarium and Mandible

Stem Cells ◽  
2006 ◽  
Vol 24 (9) ◽  
pp. 2140-2149 ◽  
Author(s):  
Mahesh H. Mankani ◽  
Sergei A. Kuznetsov ◽  
Raymond M. Wolfe ◽  
Grayson W. Marshall ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vivo Bone Formation

Therapeutic Impact of Human Bone Marrow Stromal Cells Expanded by Animal Serum–Free Medium for Cerebral Infarct in Rats

Neurosurgery ◽  
2011 ◽  
Vol 68 (6) ◽  
pp. 1733-1742 ◽  
Author(s):  
Taku Sugiyama ◽  
Satoshi Kuroda ◽  
Yukari Takeda ◽  
Mitsufumi Nishio ◽  
Masaki Ito ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Therapeutic Impact
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