Human umbilical cord mesenchymal stem cells: Osteogenesisin vivoas seed cells for bone tissue engineering

2009 ◽  
Vol 91A (1) ◽  
pp. 123-131 ◽  
Author(s):  
Yinze Diao ◽  
Qingjun Ma ◽  
Fuzhai Cui ◽  
Yanfeng Zhong
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Seed Cells

scholarly journals Layer by layer deposited osteoinductive scaffolds for bone tissue engineering

2020 ◽  
Vol 9 (2) ◽  
pp. 1089-1098
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Layer By Layer ◽  
Cissus Quadrangularis ◽  
Culture Extract

There are various bone regenerative and repair methods, but the use of osteoinductive scaffolds as bone grafts/substitute has gained wide importance worldwide. Here, an osteoinductive scaffold is developed which spontaneously stimulates stem cells to osteoblast formation without the use of any growth factor or differentiation media. We prepared electrospun PCL scaffold which is further modified for osteoinductivity by layer-by-layer method using graphene and Cissus quadrangularis callus culture extract (PCL-GP-CQ). The modified PCL-GP-CQ scaffold was compared with plain PCL scaffold and PCL coated only with GP. Physical properties such as roughness, wettability, yield strength and tensile strength of PCL-GP-CQ scaffold were found to be superior. Also, PCL-GP-CQ scaffold when seeded with human umbilical cord Wharton's jelly derived mesenchymal stem cells showed higher in vitro biocompatibility with enhanced cellular proliferation on its surface. Synergistic effect of graphene and Cissus Quadrangularis callus culture extract in scaffold boosted the differentiation of human Umbilical Cord Wharton’s jelly derived Mesenchymal Stem Cells into osteogenic lineage without any differentiation media in less than 20 days. The PCL-GP-CQ scaffold enhanced osteoblastic differentiation, osteoconduction and osteoinduction potential of scaffolds making them highly suitable for bone regeneration and bone tissue engineering applications.

hCTLA4-Gene Modified Human Bone Marrow?Derived Mesenchymal Stem Cells as Allogeneic Seed Cells in Bone Tissue Engineering

2006 ◽  
Vol 0 (0) ◽  
pp. 060913044658010
Author(s):  
Fei Dai ◽  
Dongwen Shi ◽  
Weifeng He ◽  
Jun Wu ◽  
Gaoxin Luo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Seed Cells

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

Dextran-coated fluorapatite nanorods doped with lanthanides in labelling and directing osteogenic differentiation of bone marrow mesenchymal stem cells

2014 ◽  
Vol 2 (23) ◽  
pp. 3609-3617 ◽  
Author(s):  
Haifeng Zeng ◽  
Xiyu Li ◽  
Fang Xie ◽  
Li Teng ◽  
Haifeng Chen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Novel Approach ◽  
Marrow Mesenchymal Stem Cells

A novel approach for labelling and tracking BMSCs in bone tissue engineering by using dextran-coated fluorapatite nanorods doped with lanthanides.

A Novel Strategy Incorporated the Power of Mesenchymal Stem Cells to Allografts for Segmental Bone Tissue Engineering

2010 ◽  
Vol 19 (9) ◽  
pp. 1215-1215 ◽  
Author(s):  
Xiao Hui Zou ◽  
Hong Xin Cai ◽  
Zi Yin ◽  
Xiao Chen ◽  
Yang Zi Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Segmental Bone ◽  
Novel Strategy
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