Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization

2009 ◽  
Vol 88A (4) ◽  
pp. 841-848 ◽  
Author(s):  
Rosemeire de Lordo Franco ◽  
Roberto Chiesa ◽  
Marcio Mateus Beloti ◽  
Paulo Tambasco de Oliveira ◽  
Adalberto Luiz Rosa
Keyword(s):  
Cell Response ◽  
Titanium Surface ◽  
Osteoblastic Cell ◽  
Human Osteoblastic Cell

Initial MC3T3-E1 cell response on magnesium ion implanted titanium surface

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S125
Author(s):  
H. Lee ◽  
L.-R. Cho ◽  
C.-J. Park ◽  
H. Shin
Keyword(s):  
Cell Response ◽  
Titanium Surface ◽  
Magnesium Ion ◽  
Ion Implanted

scholarly journals Immunomodulatory Potential of Differently-Terminated Ultra-Small Silicon Carbide Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 573
Author(s):  
Tereza Bělinová ◽  
Iva Machová ◽  
David Beke ◽  
Anna Fučíková ◽  
Adam Gali ◽  
...  
Keyword(s):  
Silicon Carbide ◽  
Cell Line ◽  
Metabolic Activity ◽  
Immune Cell ◽  
Cellular Membrane ◽  
Osteoblastic Cell ◽  
Osteoblastic Cell Line ◽  
Human Osteoblastic Cell ◽  
Different Response ◽  
Small Nanoparticles

Ultra-small nanoparticles with sizes comparable to those of pores in the cellular membrane possess significant potential for application in the field of biomedicine. Silicon carbide ultra-small nanoparticles with varying surface termination were tested for the biological system represented by different human cells (using a human osteoblastic cell line as the reference system and a monocyte/macrophage cell line as immune cells). The three tested nanoparticle surface terminations resulted in the observation of different effects on cell metabolic activity. These effects were mostly noticeable in cases of monocytic cells, where each type of particle caused a completely different response (‘as-prepared’ particles, i.e., were highly cytotoxic, –OH terminated particles slightly increased the metabolic activity, while –NH2 terminated particles caused an almost doubled metabolic activity) after 24 h of incubation. Subsequently, the release of cytokines from such treated monocytes and their differentiation into activated cells was determined. The results revealed the potential modulation of immune cell behavior following stimulation with particular ultra-small nanoparticles, thus opening up new fields for novel silicon carbide nanoparticle biomedical applications.

scholarly journals Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium-8tantalum-3niobium alloy

2012 ◽  
Vol 42 (6) ◽  
pp. 248 ◽  
Author(s):  
Bo-Ah Lee ◽  
Choong-Hee Kang ◽  
Mong-Sook Vang ◽  
Young-Suk Jung ◽  
Xing Hui Piao ◽  
...  
Keyword(s):  
Cell Response ◽  
Surface Characteristics ◽  
Osteoblastic Cell ◽  
Heat Treated

scholarly journals Phase-independent Inhibition by Retinoic Acid of Mineralization Correlated with Loss of Tetranectin Expression in a Human Osteoblastic Cell Line.

2001 ◽  
Vol 26 (4) ◽  
pp. 227-233 ◽  
Author(s):  
Kousuke Iba ◽  
Hideki Chiba ◽  
Toshihiko Yamashita ◽  
Seiichi Ishii ◽  
Norimasa Sawada
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Osteoblastic Cell ◽  
Osteoblastic Cell Line ◽  
Human Osteoblastic Cell

scholarly journals Connexin43 mediates direct intercellular communication in human osteoblastic cell networks.

1993 ◽  
Vol 91 (5) ◽  
pp. 1888-1896 ◽  
Author(s):  
R Civitelli ◽  
E C Beyer ◽  
P M Warlow ◽  
A J Robertson ◽  
S T Geist ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Osteoblastic Cell ◽  
Human Osteoblastic Cell ◽  
Cell Networks

Comparison of Gene-Expression of MC3T3-E1 Osteoblastic Cell Cultured on Machined Titanium Surface and Resorbable Blast Material Titanium Surface

2007 ◽  
Vol 361-363 ◽  
pp. 1095-1098
Author(s):  
S.H. Park ◽  
H.H. Kim ◽  
H.S. Lee ◽  
Y.S. Choi ◽  
A.R. Pae ◽  
...  
Keyword(s):  
Gene Expression ◽  
Titanium Surface ◽  
Cell Attachment ◽  
Single Gene ◽  
Rna Extraction ◽  
Genetic Effect ◽  
Osteoblastic Cell ◽  
Machined Surface ◽  
Osteoblastic Cell Line ◽  
Microarray Assay

Because of its high biocompatibility, hydroxyapatite(HA) has been considered as a good blasting material. DNA microarray is a new molecular technology that enables the analysis of gene expression in parallel on a very large number of genes, spanning a significant fraction of the human genome. It is a qualitive analysis (e.g. it can differentiate each single gene) and quantitative, since it has the sensitivity to detect a change of expression level in the investigated cells when compared to normal samples. The aim of this study is to define the cell attachment and the genetic effect of machined surface implant and RBM (resorbable blast media) surface implant on the osteoblastic cell (MC3T3-E1 osteoblastic cell line) by cDNA microarray slide containing 21575 genes. Cells were cultured on machined grade 4 titanium disks(Group 1, machined surface) and disks of RBM (Group 2) and the samples were moved to new dishes and media were added and the plated disks were cultured for 24 hours. Total RNA extraction was performed with Qiagen mini kit (Qiagen, Chatsworth, CA, USA) for microarray assay. Microarray assay after culturing the cells on the machined surface and RBM surface revealed that osteoinductive molecules appeared more prominent on the RBM surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than RBM surface.

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