Regulation of vascular smooth muscle cells on poly(ethylene terephthalate) film byO‐carboxymethylchitosan surface immobilization

2008 ◽  
Vol 86A (2) ◽  
pp. 467-476 ◽  
Author(s):  
Ai‐Ping Zhu ◽  
Feng Zhao ◽  
Ning Fang
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Ethylene Terephthalate ◽  
Muscle Cells ◽  
Surface Immobilization ◽  
Poly Ethylene Terephthalate ◽  
Poly Ethylene

Biomimetic strategy towards gelatin coatings on PET. Effect of protocol on coating stability and cell-interactive properties

2019 ◽  
Vol 7 (8) ◽  
pp. 1258-1269 ◽  
Author(s):  
Elena Diana Giol ◽  
Sandra Van Vlierberghe ◽  
Ronald E. Unger ◽  
Ken Kersemans ◽  
Filip de Vos ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Ethylene Terephthalate ◽  
Vascular Grafts ◽  
Muscle Cells ◽  
Poly Ethylene Terephthalate ◽  
Coating Stability ◽  
Poly Ethylene

The potential in vascular grafts of gelatin-modified poly(ethylene terephthalate) (PET) was shown herein via their coating stability, ability to promote endothelial cells (ECs) and smooth muscle cells (SMCs) and positive cyto- and endotoxicity assessments.

Isolation of Multiple Types of Plasminogen Activator Inhibitors from Vascular Smooth Muscle Cells

1989 ◽  
Vol 61 (03) ◽  
pp. 517-521 ◽  
Author(s):  
Walter E Laug ◽  
Ruedi Aebersold ◽  
Ambrose Jong ◽  
Willian Rideout ◽  
Barbara L Bergman ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Affinity Purification ◽  
Muscle Cells ◽  
Exchange Chromatography ◽  
Natural Resistance ◽  
Protease Nexin ◽  
Pai 1

SummaryLarge arteries have a natural resistance to tumor cell invasion thought to be due to the production of protease inhibitors. Vascular smooth muscle cells (VSMC) representing the major cellular part of arteries were isolated from human aortas and grown in tissue culture. These cells were found to produce large amounts of inhibitors of plasminogen activators (PA). Fractionation of VSMC-conditioned medium by heparin-affigel chromatography separated three immunologically and functionally distinct PA inhibitors (PAI), namely PAI-1, PAI-2 and protease-nexin I. The three inhibitors were characterized by functional assays and immunoblotting. PA inhibitor 2 (PAI-2) had little affinity for heparin, whereas PA inhibitor 1 (PAI-l) bound to heparin and was eluted from the column at NaCl concentrations of 0. 1 to 0.35 M. Protease-nexin I, eluted at NaCl concentrations of 0.5 M and higher. Most of the PAI-1 was present in the latent, inactive form. PAI-1 was further purified by ion exchange chromatography on a Mono-Q column. Partial sequencing of the purified PAI-1 confirmed its nature by matching completely with the sequence deduced from the cDNA nucleotide sequence of endothelial cell PAI-1. Thus, human VSMC produce all three presently known PAI and these can be separated in a single heparin affinity purification step.

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