In vitro biocompatibility of degradable biopolymers in cell line cultures from various ocular tissues: Direct contact studies

2007 ◽  
Vol 83A (2) ◽  
pp. 407-413 ◽  
Author(s):  
A. Huhtala ◽  
T. Pohjonen ◽  
L. Salminen ◽  
A. Salminen ◽  
K. Kaarniranta ◽  
...  
Keyword(s):  
Cell Line ◽  
Direct Contact ◽  
Ocular Tissues ◽  
In Vitro Biocompatibility

In vitro biocompatibility of degradable biopolymers in cell line cultures from various ocular tissues: extraction studies

2007 ◽  
Vol 19 (2) ◽  
pp. 645-649 ◽  
Author(s):  
Anne Huhtala ◽  
Timo Pohjonen ◽  
Lotta Salminen ◽  
Antero Salminen ◽  
Kai Kaarniranta ◽  
...  
Keyword(s):  
Cell Line ◽  
Ocular Tissues ◽  
In Vitro Biocompatibility

In vitro Cytotoxicity of Bisphenol A in Monocytes Cell Line

2015 ◽  
Vol 40 (2) ◽  
pp. 180-186 ◽  
Author(s):  
Mauro Neri ◽  
Grazia Maria Virzì ◽  
Alessandra Brocca ◽  
Francesco Garzotto ◽  
Jeong Chul Kim ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Cell Line ◽  
Direct Contact ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Maximum Value ◽  
In Vitro Investigation ◽  
Apoptotic Effect ◽  
Monocyte Cell

Background/Aim: Bisphenol A (BPA) is used in the production of many plastics, which are used to build biomaterials that sometimes are in direct contact with blood. It is believed that the release of BPA into bloodstream may give rise to cytotoxic events for blood components. The aim of the present study was to perform an in vitro investigation of the observable cytotoxic effect of BPA, at increasing concentrations, on the monocyte cell line. Methods: We incubated in vitro monocyte cells (U937) for 24 h in cell line medium samples (RPMI 1640) at different concentrations of BPA. We then generated curves to evaluate viability, necrosis and apoptosis of monocytes against increasing concentrations of BPA. Results: The percentage values of concentrations of BPA corresponding to 50% of the viability and necrosis of the monocytes were 1.39 and 1.48 ng/ml, respectively. Based on our observations, we reported an increasing cytotoxic effect for higher concentrations. The apoptotic effect reached the maximum value at BPA concentration of 1.5 ng/ml; at still higher concentrations, we observed a predominantly necrotic cell death. Conclusion: Viability, necrosis and apoptosis of monocytes are strongly and positively correlated with BPA concentration. A direct contact of such compound with biological components of blood may lead to high levels of cytotoxicity, and require us to evaluate additional factors while judging the bio-incompatibility of BPA.

scholarly journals Influence of ZnO thin film crystallinity on in vitro biocompatibility

2018 ◽  
Vol 7 (5) ◽  
pp. 754-759 ◽  
Author(s):  
Nastassja A. Lewinski ◽  
Vitaliy Avrutin ◽  
Tanin Izadi ◽  
Lynn E. Secondo ◽  
Md. Barkat Ullah ◽  
...  
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Direct Contact ◽  
Zno Thin Films ◽  
Zno Thin Film ◽  
In Vitro Biocompatibility

This study evaluated the cytocompatibility of single- and poly-crystalline ZnO thin films using extract and direct contact methods.

In vitro biocompatibility evaluation of biscoumarin based random copolyesters

2015 ◽  
Vol 39 (6) ◽  
pp. 4948-4956 ◽  
Author(s):  
Kandaswamy Narendran ◽  
Raveendiran Nanthini
Keyword(s):  
Cell Line ◽  
Normal Cell ◽  
Vero Cells ◽  
Normal Cell Line ◽  
In Vitro Biocompatibility

Copolyester CP5 exhibits cytocompatible properties toward a normal cell line (Vero cells) and requires 13-fold higher concentration in comparison with Hep-2 cells.

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

ANTITUMOR EFFECT OF SUNITINIB AND BORTEZOMIB ON BREAST CANCER CELL LINE IN VITRO

2017 ◽  
Vol 63 (1) ◽  
pp. 141-145
Author(s):  
Yuliya Khochenkova ◽  
Eliso Solomko ◽  
Oksana Ryabaya ◽  
Yevgeniya Stepanova ◽  
Dmitriy Khochenkov
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Antitumor Effect ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Actual Problem

The discovery for effective combinations of anticancer drugs for treatment for breast cancer is the actual problem in the experimental chemotherapy. In this paper we conducted a study of antitumor effect of the combination of sunitinib and bortezomib against MDA-MB-231 and SKBR-3 breast cancer cell lines in vitro. We found that bortezomib in non-toxic concentrations can potentiate the antitumor activity of sunitinib. MDA-MB-231 cell line has showed great sensitivity to the combination of bortezomib and sunitinib in vitro. Bortezomib and sunitinib caused reduced expression of receptor tyrosine kinases VEGFR1, VEGFR2, PDGFRa, PDGFRß and c-Kit on HER2- and HER2+ breast cancer cell lines

New Platinum (II) Ternary Complexes of Formamidine and Pyrophosphate: Synthesis, Characterization and DFT Calculations and In vitro Cytotoxicity

2020 ◽  
Vol 23 (7) ◽  
pp. 611-623
Author(s):  
Ahmed A. Soliman ◽  
Fawzy A. Attaby ◽  
Othman I. Alajrawy ◽  
Azza A.A. Abou-hussein ◽  
Wolfgang Linert
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Theoretical Calculations ◽  
Thermal Analyses ◽  
Cancer Cell Line ◽  
Cellular Growth ◽  
Planar Geometry ◽  
Square Planar ◽  
Vis Spectroscopy

Aim and Objective: Platinum (II) and platinum (IV) of pyrophosphate complexes have been prepared and characterized to discover their potential as antitumor drugs. This study was conducted to prepare and characterize new ternary platinum (II) complexes with formamidine and pyrophosphate as an antitumor candidate. Materials and Methods: The complexes have been characterized by mass, infrared, UV-Vis. spectroscopy, elemental analysis, magnetic susceptibility, thermal analyses, and theoretical calculations. They have been tested for their cytotoxicity, which was carried out using the fastcolorimetric assay for cellular growth and survival against MCF-7 (breast cancer cell line), HCT- 116 (colon carcinoma cell line), and HepG-2 (hepatocellular cancer cell line). Results: All complexes are diamagnetic, and the electronic spectral data displayed the bands due to square planar Pt(II) complexes. The optimized complexes structures (1-4) indicated a distorted square planar geometry where O-Pt-O and N-Pt-N bond angles were 82.04°-96.44°, respectively. Conclusion: The complexes showed noticeable cytotoxicity and are considered as promising antitumor candidates for further applications.

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