Substrate porosity enhances chondrocyte attachment, spreading, and cartilage tissue formation in vitro

2006 ◽  
Vol 78A (4) ◽  
pp. 676-683 ◽  
Author(s):  
C. G. Spiteri ◽  
R. M. Pilliar ◽  
R. A. Kandel
Keyword(s):  
Cartilage Tissue ◽  
Tissue Formation ◽  
And Cartilage

Synovial Mesenchymal Stem Cells Derived From the Cotyloid Fossa Synovium Have Higher Self-renewal and Differentiation Potential Than Those From the Paralabral Synovium in the Hip Joint

2018 ◽  
Vol 46 (12) ◽  
pp. 2942-2953 ◽  
Author(s):  
Yoichi Murata ◽  
Soshi Uchida ◽  
Hajime Utsunomiya ◽  
Akihisa Hatakeyama ◽  
Hirotaka Nakashima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Femoroacetabular Impingement ◽  
Impingement Syndrome ◽  
Cartilage Tissue ◽  
Gene Expressions ◽  
Differentiation Potential ◽  
Femoroacetabular Impingement Syndrome ◽  
And Cartilage

Background: Several studies have shown the relationship between poorer clinical outcomes of arthroscopic femoroacetabular impingement syndrome surgery and focal chondral defects or global chondromalacia/osteoarthritis. Although recent studies described good outcomes after the conjunctive application of synovial mesenchymal stem cells (MSCs), none demonstrated the application of synovial MSCs for cartilaginous hip injuries. Purpose: To compare the characteristics of MSCs derived from the paralabral synovium and the cotyloid fossa synovium and determine which is the better source. Study Design: Controlled laboratory study. Methods: Synovium was harvested from 2 locations of the hip—paralabral and cotyloid fossa—from 18 donors. The number of cells, colony-forming units, viability, and differentiation capacities of adipose, bone, and cartilage were collected and compared between groups. In addition, real-time polymerase chain reaction was used to assess the differentiation capacity of adipose, bone, and cartilage tissue from both samples. Results: The number of colonies and yield obtained at passage 0 of synovium from the cotyloid fossa was significantly higher than that of the paralabral synovium ( P < .01). In adipogenesis experiments, the frequency of detecting oil red O–positive colonies was significantly higher in the cotyloid fossa than in the paralabral synovium ( P < .05). In osteogenesis experiments, the frequency of von Kossa and alkaline phosphatase positive colonies was higher in the cotyloid fossa synovium than in the paralabral synovium ( P < .05). In chondrogenic experiments, the chondrogenic pellet culture and the gene expressions of COL2a1 and SOX9 were higher in the cotyloid fossa synovium than in the paralabral synovium ( P < .05). Conclusion: MSCs from the cotyloid fossa synovium have higher proliferation and differentiation potential than do those from the paralabral synovium and are therefore a better source. Clinical Relevance: Synovial cells from the cotyloid fossa synovium of patients with femoroacetabular impingement syndrome are more robust in vitro, suggesting that MSCs from this source may be strongly considered for stem cell therapy.

scholarly journals In Vitro Fabrication of Hybrid Bone/Cartilage Complex Using Mouse Induced Pluripotent Stem Cells

2020 ◽  
Vol 21 (2) ◽  
pp. 581 ◽  
Author(s):  
Phoonsuk Limraksasin ◽  
Takeru Kondo ◽  
Maolin Zhang ◽  
Hiroko Okawa ◽  
Thanaphum Osathanon ◽  
...  
Keyword(s):  
Cartilage Tissue ◽  
Marker Genes ◽  
Induction Medium ◽  
Mechanical Stimuli ◽  
Tissue Formation ◽  
Osteogenic Induction Medium ◽  
Osteogenic Induction ◽  
Chondrogenic Marker ◽  
Induced Pluripotent

Cell condensation and mechanical stimuli play roles in osteogenesis and chondrogenesis; thus, they are promising for facilitating self-organizing bone/cartilage tissue formation in vitro from induced pluripotent stem cells (iPSCs). Here, single mouse iPSCs were first seeded in micro-space culture plates to form 3-dimensional spheres. At day 12, iPSC spheres were subjected to shaking culture and maintained in osteogenic induction medium for 31 days (Os induction). In another condition, the osteogenic induction medium was replaced by chondrogenic induction medium at day 22 and maintained for a further 21 days (Os-Chon induction). Os induction produced robust mineralization and some cartilage-like tissue, which promoted expression of osteogenic and chondrogenic marker genes. In contrast, Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue, with greatly increased expression of chondrogenic marker genes along with osterix and collagen 1a1. Os-Chon induction enhanced mesodermal lineage commitment with brachyury expression followed by high expression of lateral plate and paraxial mesoderm marker genes. These results suggest that combined use of micro-space culture and mechanical stimuli facilitates hybrid bone/cartilage tissue formation from iPSCs, and that the bone/cartilage tissue ratio in iPSC constructs could be manipulated through the induction protocol.

scholarly journals Dual Delivery of TGF-β3 and Ghrelin in Microsphere/Hydrogel Systems for Cartilage Regeneration

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5732
Author(s):  
Jianjing Lin ◽  
Li Wang ◽  
Jianhao Lin ◽  
Qiang Liu
Keyword(s):  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Transforming Growth Factor ◽  
Double Emulsion ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Self Healing ◽  
Tissue Formation ◽  
Extraction Technology

Articular cartilage (AC) damage is quite common, but due to AC’s poor self-healing ability, the damage can easily develop into osteoarthritis (OA). To solve this problem, we developed a microsphere/hydrogel system that provides two growth factors that promote cartilage repair: transforming growth factor-β3 (TGF-β3) to enhance cartilage tissue formation and ghrelin synergy TGF-β to significantly enhance the chondrogenic differentiation. The hydrogel and microspheres were characterized in vitro, and the biocompatibility of the system was verified. Double emulsion solvent extraction technology (w/o/w) is used to encapsulate TGF-β3 and ghrelin into microspheres, and these microspheres are encapsulated in a hydrogel to continuously release TGF-β3 and ghrelin. According to the chondrogenic differentiation ability of mesenchymal stem cells (MSCs) in vitro, the concentrations of the two growth factors were optimized to promote cartilage regeneration.

scholarly journals Impact of dexamethasone concentration on cartilage tissue formation from human synovial derived stem cells in vitro

2018 ◽  
Vol 70 (2) ◽  
pp. 819-829 ◽  
Author(s):  
Ryota Chijimatsu ◽  
Masato Kobayashi ◽  
Kosuke Ebina ◽  
Toru Iwahashi ◽  
Yosuke Okuno ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cartilage Tissue ◽  
Tissue Formation

scholarly journals Combinatorial screening of biochemical and physical signals for phenotypic regulation of stem cell–based cartilage tissue engineering

2020 ◽  
Vol 6 (21) ◽  
pp. eaaz5913 ◽  
Author(s):  
Junmin Lee ◽  
Oju Jeon ◽  
Ming Kong ◽  
Amr A. Abdeen ◽  
Jung-Youn Shin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Articular Cartilage ◽  
Wnt Signaling Pathway ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Design Strategies ◽  
Rgd Peptides

Despite great progress in biomaterial design strategies for replacing damaged articular cartilage, prevention of stem cell-derived chondrocyte hypertrophy and resulting inferior tissue formation is still a critical challenge. Here, by using engineered biomaterials and a high-throughput system for screening of combinatorial cues in cartilage microenvironments, we demonstrate that biomaterial cross-linking density that regulates matrix degradation and stiffness—together with defined presentation of growth factors, mechanical stimulation, and arginine-glycine-aspartic acid (RGD) peptides—can guide human mesenchymal stem cell (hMSC) differentiation into articular or hypertrophic cartilage phenotypes. Faster-degrading, soft matrices promoted articular cartilage tissue formation of hMSCs by inducing their proliferation and maturation, while slower-degrading, stiff matrices promoted cells to differentiate into hypertrophic chondrocytes through Yes-associated protein (YAP)–dependent mechanotransduction. in vitro and in vivo chondrogenesis studies also suggest that down-regulation of the Wingless and INT-1 (WNT) signaling pathway is required for better quality articular cartilage-like tissue production.

Cartilaginous Tissue Formation with an Elastic PLCL Scaffold and Human Adipose Tissue-Derived Stromal Cells

2007 ◽  
Vol 342-343 ◽  
pp. 385-388
Author(s):  
So Eun Lee ◽  
Young Mee Jung ◽  
Soo Hyun Kim ◽  
Sang Heon Kim ◽  
Jong Won Rhie ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Cartilage Tissue Engineering ◽  
Human Adipose Tissue ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Cartilaginous Tissue

In cartilage tissue engineering, as a cell source, adult stem cells are very attractive for clinical applications. Recent studies suggest that human adipose tissue-derived stromal cells (ASCs) have multilineage potential similar to bone marrow-derived stromal cells (BMSCs). ASCs are obtained from adipose tissue easily isolated by suction-assisted lipectomy in various body parts. Also, as one of major factors of cartilage tissue engineering, scaffolds have an important role in cartilage formation. Poly(L-lactide-co-ε-carprolactone) scaffolds have physiological activity, biodegradability, high cell affinity, and mechano-activity. The object of this study is cartilaginous tissue formation using highly elastic PLCL scaffolds and ASCs in vitro and in vivo. Poly(L-lactide-co-ε-carprolactone) copolymers were synthesized from lactide and ε-carprolactone in the presence of stannous octoate as catalyst. The scaffolds with 85% porosity and 300-500μm pore size were fabricated by gel-pressing method. ASCs were seeded on scaffolds and cultured for 21days in vitro. Cell/polymer constructs were characterized by reverse transcriptase-polymerase chain reaction for confirming differentiation to chondrocytes onto PLCL scaffolds. Also, for examining cartilaginous tissue formation in vivo, ASCs seeded scaffolds which were induced chondrogenesis for 2 weeks were implanted in nude mice subcutaneously for up to 8weeks. Histological studies showed that implants partially developed cartilaginous tissue within lacunae. And there was an accumulation of sulfated glycoaminoglycans. Immunohistochemical analysis revealed that implants were positively stained for specific extracellular matrix. These results indicate that ASCs and PLCL scaffols could be used to cartilage tissue engineering.

scholarly journals Probiotics Composition Harbors Against Osteoarthritis and Inflammation through GABA in Mice

2022 ◽  
Author(s):  
Uzma Amin ◽  
Rong Jiang ◽  
Shahid Masood Raza ◽  
Li Liang ◽  
Naibo Feng ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Cruciate Ligament ◽  
Cartilage Tissue ◽  
Protective Effects ◽  
Chondrocyte Maturation ◽  
Joint Axis ◽  
Age Related ◽  
And Cartilage

Abstract Background: Osteoarthritis (OA) is an age-related disease with multifactorial etiology and its prevalence growing globally. The role of Gut microbiota is inevitable concerning musculoskeletal disease and health. A method of controlling inflammation and cartilage destruction through changes in gut microbiota is proposed. Previously reported data lack the specific approach to microbial clusters and biomarkers in understanding the interactions between host and microbiome.Method: We adopted a novel approach to elaborate the positive influence of S. thermophilus and L. pentosus to treat Anterior cruciate ligament transection (ACLT) induced OA in vivo. For in vitro analysis Human Chondrocyte Cell Line (C28/I2) was used to analyze chondrogenic effect of microbes and GABA. Tukey’s multiple-comparisons test or Two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli test were used to statistically analyze the data.Results: The gut microbiota-joint axis promoted chondrogenesis and inhibited catabolism. Selected bacteria produced GABA as postbiotic. This study is the first to represent the chondrogenic and protective effects of γ-aminobutyric acid (GABA) on human chondrocytes and cartilage tissue in mice. Oral administration of it down-regulated cartilage degradation in OA-induced mice and decreased inflammation.Conclusion: We speculated the positive results from GABA and probiotics producing GABA against OA. GABA may have functional roles in chondrocyte maturation /differentiation. This data provides a foundation for further studies to elucidate the role of GABA producing microbes and GABA in the regulation of cartilaginous cell proliferation. These findings open future horizons to understand the gut-joint axis and for the treatment of OA. Thus probiotic / GABA therapy could act as a nutraceutical modulator for OA.

scholarly journals A Pilot Study of Seamless Regeneration of Bone and Cartilage in Knee Joint Regeneration Using Honeycomb TCP

Materials ◽  
2021 ◽  
Vol 14 (23) ◽  
pp. 7225
Author(s):  
Kiyofumi Takabatake ◽  
Hidetsugu Tsujigiwa ◽  
Aki Yoshida ◽  
Takayuki Furumatsu ◽  
Hotaka Kawai ◽  
...  
Keyword(s):  
Knee Joint ◽  
Tissue Regeneration ◽  
Transforming Growth Factor ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Defect Model ◽  
Regeneration Of Bone ◽  
Bone Tissue Formation ◽  
And Cartilage

The knee joint is a continuous structure of bone and cartilage tissue, making it difficult to regenerate using artificial biomaterials. In a previous study, we succeeded in developing honeycomb tricalcium phosphate (TCP), which has through-and-through holes and is able to provide the optimum microenvironment for hard tissue regeneration. We demonstrated that TCP with 300 μm pore diameters (300TCP) induced vigorous bone formation, and that TCP with 75 μm pore diameters (75TCP) induced cartilage formation. In the present study, we regenerated a knee joint defect using honeycomb TCP. 75TCP and 300TCP were loaded with transforming growth factor (TGF)-β alone or bone morphogenic protein (BMP)-2+TGF-β with or without Matrigel and transplanted into knee joint defect model rabbits. 75TCP showed no bone or cartilage tissue formation in any of the groups with TGF-β alone and BMP-2+TGF-β with/without Matrigel. However, for 300TCP and BMP-2+TGF-β with or without Matrigel, vigorous bone tissue formation was observed in the TCP holes, and cartilage tissue formation in the TCP surface layer was continuous with the existing cartilage. The cartilage area in the TCP surface was larger in the group without Matrigel (with BMP-2+TGF-β) than in the group with Matrigel (with BMP-2+TGF-β). Therefore, honeycomb TCP can induce the seamless regeneration of bone and cartilage in a knee joint.

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