Tissue engineered cartilage using human articular chondrocytes immortalized by HPV-16 E6 and E7 genes

2006 ◽  
Vol 76A (3) ◽  
pp. 512-520 ◽  
Author(s):  
Wei-Hung Chen ◽  
Wen-Fu Lai ◽  
Win-Ping Deng ◽  
Wen K. Yang ◽  
Wen-Cheng Lo ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Human Articular Chondrocytes ◽  
Hpv 16 ◽  
E6 And E7 ◽  
Engineered Cartilage

scholarly journals Human articular chondrocytes immortalized by HPV-16 E6 and E7 genes:

2002 ◽  
Vol 10 (11) ◽  
pp. 879-889 ◽  
Author(s):  
B. Grigolo ◽  
L. Roseti ◽  
S. Neri ◽  
P. Gobbi ◽  
P. Jensen ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Human Articular Chondrocytes ◽  
Hpv 16 ◽  
E6 And E7

Collagen Expression in Tissue Engineered Cartilage of Aged Human Articular Chondrocytes in a Rotating Bioreactor

2003 ◽  
Vol 26 (4) ◽  
pp. 319-330 ◽  
Author(s):  
S. Marlovits ◽  
B. Tichy ◽  
M. Truppe ◽  
D. Gruber ◽  
W. Schlegel
Keyword(s):  
Electron Microscopy ◽  
Collagen Type ◽  
Articular Chondrocytes ◽  
Three Dimensional ◽  
Collagen Type I ◽  
Human Articular Chondrocytes ◽  
Type I ◽  
Transmission Electron ◽  
Low Shear Stress ◽  
Engineered Cartilage

This study describes the culture and three-dimensional assembly of aged human articular chondrocytes under controlled oxygenation and low shear stress in a rotating-wall vessel. Chondrocytes cultured in monolayer were released and placed without any scaffold as a single cell suspension in a rotating bioreactor for 12 weeks. Samples were analyzed with immunohistochemistry, molecular biology and electron microscopy. During serial monolayer cultures chondrocytes dedifferentiated to a “fibroblast-like” structure and produced predominantly collagen type I. When these dedifferentiated cells were transferred to the rotating bioreactor, the cells showed a spontaneous aggregation and formation of solid tissue during the culture time. Expression of collagen type II and other components critical for the extracellular cartilage matrix could be detected. Transmission electron microscopy revealed a fine network of randomly distributed collagen fibrils. This rotating bioreactor proves to be a useful tool for providing an environment that enables dedifferentiated chondrocytes to redifferentiate and produce a cartilage-specific extracellular matrix.

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