Human endothelial cell attachment and proliferation on a novel vascular graft prototype

2004 ◽  
Vol 71A (4) ◽  
pp. 615-624 ◽  
Author(s):  
Menno L. W. Knetsch ◽  
Yvette B. J. Aldenhoff ◽  
Martijn Schraven ◽  
Leo H. Koole
Keyword(s):  
Endothelial Cell ◽  
Vascular Graft ◽  
Cell Attachment ◽  
Human Endothelial Cell ◽  
Endothelial Cell Attachment

Serum enhancement of human endothelial cell attachment to and spreading on collagens I and IV does not require serum fibronectin or vitronectin

1990 ◽  
Vol 95 (2) ◽  
pp. 255-262
Author(s):  
W.D. Norris ◽  
J.G. Steele ◽  
G. Johnson ◽  
P.A. Underwood
Keyword(s):  
Endothelial Cell ◽  
Culture Medium ◽  
Type I Collagen ◽  
Cell Attachment ◽  
Cell Spreading ◽  
Human Endothelial Cell ◽  
Collagen Type Iv ◽  
Type I ◽  
Type Iv ◽  
Endothelial Cell Attachment

The initial attachment and spreading of endothelial cells from human umbilical artery onto type I collagen, type IV collagen or gelatin substrata was shown to be enhanced by inclusion of serum in the culture medium. To test whether this serum effect was mediated by adsorption of serum fibronectin or vitronectin onto the collagen, these adhesive glycoproteins were selectively removed from the serum prior to addition to the culture medium. The stimulatory effect of serum on human endothelial cell spreading on collagens I and IV was also observed with serum from which either fibronectin or vitronectin, or both, had been selectively removed. The stimulatory effect for cell spreading on gelatin was diminished by selective removal of serum fibronectin, but unaffected by removal of vitronectin. Human endothelial cell attachment and spreading onto tissue culture plastic was abolished by removal of vitronectin from the serum in the culture medium. These results emphasize that the native structure of collagens is required for serum-enhancement of human endothelial cell attachment and spreading on native collagen types I and IV, and show that on these substrata the stimulated adhesion and spreading are not dependent upon adsorption of serum fibronectin or vitronectin onto the collagen substratum.

scholarly journals Bone Sialoprotein Mediates Human Endothelial Cell Attachment and Migration and Promotes Angiogenesis

2000 ◽  
Vol 86 (8) ◽  
pp. 885-891 ◽  
Author(s):  
Akeila Bellahcène ◽  
Karine Bonjean ◽  
Berthold Fohr ◽  
Neal S. Fedarko ◽  
Frank A. Robey ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Attachment ◽  
Bone Sialoprotein ◽  
Human Endothelial Cell ◽  
Endothelial Cell Attachment ◽  
And Migration

Naturally Produced Extracellular Matrix Is an Excellent Substrate for Canine Endothelial Cell Proliferation and Resistance to Shear Stress on PTFE Vascular Grafts

1997 ◽  
Vol 78 (05) ◽  
pp. 1392-1398 ◽  
Author(s):  
A Schneider ◽  
M Chandra ◽  
G Lazarovici ◽  
I Vlodavsky ◽  
G Merin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Attachment ◽  
Thrombus Formation ◽  
Endothelial Cell Proliferation ◽  
Ptfe Graft ◽  
Vascular Prostheses ◽  
Endothelial Cell Attachment

SummaryPurpose: Successful development of a vascular prosthesis lined with endothelial cells (EC) may depend on the ability of the attached cells to resist shear forces after implantation. The present study was designed to investigate EC detachment from extracellular matrix (ECM) precoated vascular prostheses, caused by shear stress in vitro and to test the performance of these grafts in vivo. Methods: Bovine aortic endothelial cells were seeded inside untreated polytetrafluoro-ethylene (PTFE) vascular graft (10 X 0.6 cm), PTFE graft precoated with fibronectin (FN), or PTFE precoated with FN and a naturally produced ECM (106 cells/graft). Sixteen hours after seeding the medium was replaced and unattached cells counted. The strength of endothelial cell attachment was evaluated by subjecting the grafts to a physiologic shear stress of 15 dynes/cm2 for 1 h. The detached cells were collected and quantitated. PTFE or EC preseeded ECM coated grafts were implanted in the common carotid arteries of dogs. Results: While little or no differences were found in the extent of endothelial cell attachment to the various grafts (79%, 87% and 94% of the cells attached to PTFE, FN precoated PTFE, or FN+ECM precoated PTFE, respectively), the number of cells retained after a shear stress was significanly increased on ECM coated PTFE (20%, 54% and 85% on PTFE, FN coated PTFE, and FN+ECM coated PTFE, respectively, p <0.01). Implantation experiments in dogs revealed a significant increase in EC coverage and a reduced incidence of thrombus formation on ECM coated grafts that were seeded with autologous saphenous vein endothelial cells prior to implantation. Conclusion: ECM coating significantly increased the strength of endothelial cell attachment to vascular prostheses subjected to shear stress. The presence of adhesive macromolecules and potent endothelial cell growth promoting factors may render the ECM a promising substrate for vascular prostheses.

scholarly journals Endothelial cell attachment and shear response on biomimetic polymer-coated vascular grafts

2012 ◽  
Vol 100A (8) ◽  
pp. 2204-2210 ◽  
Author(s):  
Lynn A. Dudash ◽  
Faina Kligman ◽  
Samantha M. Sarett ◽  
Kandice Kottke-Marchant ◽  
Roger E. Marchant
Keyword(s):  
Endothelial Cell ◽  
Cell Attachment ◽  
Vascular Grafts ◽  
Shear Response ◽  
Biomimetic Polymer ◽  
Endothelial Cell Attachment

scholarly journals Comparison of endothelial cell attachment on surfaces of biodegradable polymer-coated magnesium alloys in a microfluidic environment

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0205611 ◽  
Author(s):  
Lumei Liu ◽  
Sang-Ho Ye ◽  
Xinzhu Gu ◽  
Teal Russell ◽  
Zhigang Xu ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Magnesium Alloys ◽  
Biodegradable Polymer ◽  
Cell Attachment ◽  
Endothelial Cell Attachment
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