Casual cell surface remodeling using biocompatible lipid-poly(ethylene glycol)(n): Development of stealth cells and monitoring of cell membrane behavior in serum-supplemented conditions

2004 ◽  
Vol 70A (2) ◽  
pp. 179-185 ◽  
Author(s):  
Hyeyoung A. Chung ◽  
Koich Kato ◽  
Chika Itoh ◽  
Syunsuke Ohhashi ◽  
Teruyuki Nagamune
Keyword(s):  
Cell Membrane ◽  
Cell Surface ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Membrane Behavior ◽  
Poly Ethylene ◽  
Surface Remodeling

scholarly journals Haemolytic activity of stonustoxin from stonefish (Synanceja horrida) venom: pore formation and the role of cationic amino acid residues

1997 ◽  
Vol 325 (3) ◽  
pp. 685-691 ◽  
Author(s):  
Desong CHEN ◽  
R. Manjunatha KINI ◽  
Raymond YUEN ◽  
Hoon Eng KHOO
Keyword(s):  
Cell Membrane ◽  
Ethylene Glycol ◽  
Haemolytic Activity ◽  
Side Chains ◽  
Poly Ethylene Glycol ◽  
Arginine Residues ◽  
Poly Ethylene ◽  
Cationic Residues ◽  
Positively Charged

Stonustoxin (SNTX) is a two-subunit protein toxin purified from the venom of the stonefish (Synanceja horrida), which induces potent haemolytic activity. We examined the pore-forming property of this non-enzymic protein by an osmotic protection assay. SNTX-induced haemolysis was completely prevented by osmotic protectants of adequate size [poly(ethylene) glycol 3000; molecular diameter approx. 3.2 nm]. Uncharged molecules of smaller size, such as raffinose and poly(ethylene) glycol 1000–2000, failed to protect against cell lysis. These findings indicate that SNTX induces the formation of hydrophilic pores in the cell membrane, which results in the lysis of erythrocytes. Since cationic residues contribute significantly to the cytolytic activity of several other pore-forming toxins, we examined the role of positively charged lysine and arginine residues in the haemolytic activity of SNTX. SNTX lost its haemolytic activity when the positively charged side chains of lysine residues were neutralized or converted into negatively charged side chains upon carbamylation or succinylation respectively. The haemolytic activity of SNTX was also inhibited by the modification of positively charged arginine residues using 2,3-butanedione. The loss of haemolysis showed strong correlation with the number of Lys or Arg residues modified. CD analyses, however, showed that the conformation of SNTX was not significantly affected by these chemical modifications. Further, the haemolytic activity of SNTX was competitively inhibited by various negatively charged lipids, such as phosphatidylserine, cardiolipin and monosialogangliosides. These results indicate that SNTX induces potent haemolytic activity through the formation of pores in the cell membrane, and that cationic residues play a crucial role in its cytolytic mechanism.

Protein cell-surface display through in situ enzymatic modification of proteins with a poly(Ethylene glycol)-lipid

2013 ◽  
Vol 110 (10) ◽  
pp. 2785-2789 ◽  
Author(s):  
Urara Tomita ◽  
Satoshi Yamaguchi ◽  
Yasukazu Maeda ◽  
Kazuki Chujo ◽  
Kosuke Minamihata ◽  
...  
Keyword(s):  
Cell Surface ◽  
Ethylene Glycol ◽  
Surface Display ◽  
Cell Surface Display ◽  
Enzymatic Modification ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Protein Cell

Optimizing Cell−Surface Interactions by Photografting of Poly(ethylene glycol)

Langmuir ◽  
2000 ◽  
Vol 16 (6) ◽  
pp. 2756-2765 ◽  
Author(s):  
V. H. Thom ◽  
G. Altankov ◽  
Th. Groth ◽  
K. Jankova ◽  
G. Jonsson ◽  
...  
Keyword(s):  
Cell Surface ◽  
Ethylene Glycol ◽  
Surface Interactions ◽  
Poly Ethylene Glycol ◽  
Cell Surface Interactions ◽  
Poly Ethylene

scholarly journals Drug Release by Direct Jump from Poly(ethylene-glycol-b-ε-caprolactone) Nano-Vector to Cell Membrane

Molecules ◽  
2016 ◽  
Vol 21 (12) ◽  
pp. 1643 ◽  
Author(s):  
Ugo Till ◽  
Laure Gibot ◽  
Anne-Françoise Mingotaud ◽  
Jérôme Ehrhart ◽  
Luc Wasungu ◽  
...  
Keyword(s):  
Drug Release ◽  
Cell Membrane ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Cell surface pH imaging using poly(ethylene glycol)-phospholipid: its potential as the core structure of membrane anchored-probes

2019 ◽  
Vol 153 (6) ◽  
pp. 254-260
Author(s):  
Ryuichi Ohgaki ◽  
Yuji Teramura ◽  
Daichi Hayashi ◽  
Shushi Nagamori ◽  
Madoka Takai ◽  
...  
Keyword(s):  
Cell Surface ◽  
Ethylene Glycol ◽  
Core Structure ◽  
Poly Ethylene Glycol ◽  
Surface Ph ◽  
The Core ◽  
Ph Imaging ◽  
Poly Ethylene
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