Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres

2003 ◽  
Vol 65A (4) ◽  
pp. 489-497 ◽  
Author(s):  
Anat Perets ◽  
Yaacov Baruch ◽  
Felix Weisbuch ◽  
Gideon Shoshany ◽  
Gera Neufeld ◽  
...  
Keyword(s):  
Controlled Release ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Three Dimensional ◽  
Fibroblast Growth

scholarly journals Stabilization and controlled-release of basic fibroblast growth factor by water-insoluble aluminum salts of cyclodextrin sulfates.

1994 ◽  
Vol 9 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Kazuhiro Fukunaga ◽  
Kimihiro Ishimura ◽  
Shigeki Hijikata ◽  
Ryouichi Sonoda ◽  
Tetsumi Irie ◽  
...  
Keyword(s):  
Controlled Release ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Aluminum Salts

scholarly journals Porous Silk Fibroin Microspheres Sustainably Releasing Bioactive Basic Fibroblast Growth Factor

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1280 ◽  
Author(s):  
Jing Qu ◽  
Lu Wang ◽  
Longxing Niu ◽  
Jiaming Lin ◽  
Qian Huang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Silk Fibroin ◽  
Basic Fibroblast Growth Factor ◽  
Three Dimensional ◽  
Burst Release ◽  
Fibroblast Growth ◽  
Cell Viability Assay ◽  
Promote Cell Proliferation

Basic fibroblast growth factor (bFGF) plays a significant role in stimulating cell proliferation. It remains a challenge in the field of biomaterials to develop a carrier with the capacity of continuously releasing bioactive bFGF. In this study, porous bFGF-loaded silk fibroin (SF) microspheres, with inside-out channels, were fabricated by high-voltage electrostatic differentiation, and followed by lyophilization. The embedded bFGF exhibited a slow release mode for over 13 days without suffering burst release. SEM observations showed that incubated L929 cells could fully spread and produce collagen-like fibrous matrix on the surface of SF microspheres. CLSM observations and the results of cell viability assay indicated that bFGF-loaded microspheres could significantly promote cell proliferation during five to nine days of culture, compared to bFGF-unloaded microspheres. This reveals that the bFGF released from SF microspheres retained obvious bioactivity to stimulate cell growth. Such microspheres sustainably releasing bioactive bFGF might be applied to massive cell culture and tissue engineering as a matrix directly, or after being combined with three-dimensional scaffolds.

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