Proanthocyanidin: A natural crosslinking reagent for stabilizing collagen matrices

2003 ◽  
Vol 65A (1) ◽  
pp. 118-124 ◽  
Author(s):  
Bo Han ◽  
Jason Jaurequi ◽  
Bao Wei Tang ◽  
Marcel E. Nimni
Keyword(s):  
Collagen Matrices ◽  
Crosslinking Reagent

Biofilm-formation ability of S. aureus on collagen matrices

2020 ◽  
Vol 26 (5) ◽  
pp. 26-31
Author(s):  
O. Iungin ◽  
L. Maistrenko ◽  
P. Rebrykova ◽  
I. Duka
Keyword(s):  
Biofilm Formation ◽  
Collagen Matrices

scholarly journals Dendritic Fibroblasts in Three-dimensional Collagen Matrices

2003 ◽  
Vol 14 (2) ◽  
pp. 384-395 ◽  
Author(s):  
Frederick Grinnell ◽  
Chin-Han Ho ◽  
Elisa Tamariz ◽  
David J. Lee ◽  
Gabriella Skuta
Keyword(s):  
Dimensional Space ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Rho Kinase ◽  
Matrix Remodeling ◽  
Dependent Manner ◽  
Collagen Matrices ◽  
Form And Function ◽  
Metabolic Coupling ◽  
Dendritic Network

Cell motility determines form and function of multicellular organisms. Most studies on fibroblast motility have been carried out using cells on the surfaces of culture dishes. In situ, however, the environment for fibroblasts is the three-dimensional extracellular matrix. In the current research, we studied the morphology and motility of human fibroblasts embedded in floating collagen matrices at a cell density below that required for global matrix remodeling (i.e., contraction). Under these conditions, cells were observed to project and retract a dendritic network of extensions. These extensions contained microtubule cores with actin concentrated at the tips resembling growth cones. Platelet-derived growth factor promoted formation of the network; lysophosphatidic acid stimulated its retraction in a Rho and Rho kinase-dependent manner. The dendritic network also supported metabolic coupling between cells. We suggest that the dendritic network provides a mechanism by which fibroblasts explore and become interconnected to each other in three-dimensional space.

Toward reconstruction of the subcutaneous fat layer with the use of adipose-derived stromal cell–seeded collagen matrices

Cytotherapy ◽  
2014 ◽  
Vol 16 (12) ◽  
pp. 1700-1708 ◽  
Author(s):  
Katharina Werner ◽  
Michael G. Jakubietz ◽  
Rafael G. Jakubietz ◽  
Karsten Schmidt ◽  
Christian Muhr ◽  
...  
Keyword(s):  
Stromal Cell ◽  
Subcutaneous Fat ◽  
Collagen Matrices

Influence of Collagen Status on Microstructures of Porous Collagen/TCP Composites

2007 ◽  
Vol 330-332 ◽  
pp. 495-498
Author(s):  
Chao Zou ◽  
Wen Jian Weng ◽  
Xu Liang Deng ◽  
Kui Cheng ◽  
Pi Yi Du ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
Tricalcium Phosphate ◽  
Freeze Drying ◽  
Alkali Treatment ◽  
Test Results ◽  
Collagen Matrices ◽  
The Difference ◽  
Porous Composites ◽  
Better Than

Two starting collagens, sponge and floc collagen, were used to prepare collagen/tricalcium phosphate (TCP) composites. The resulting composites were porous and had 200μm pore size. However, there was a difference in the microstructure of the pore walls for the composites derived from the two collagens, the pore walls in sponge collagen/TCP composite were still porous and had 200 nm micropores size, TCP particles were trapped in collagen matrices. While floc collagen/TCP composite had smooth and dense walls in which TCP particles were embedded. The difference could be attributed to the starting collagen with different status. Sponge collagen has a soft structure, which easily becomes disassembled fibrils during alkali treatment, the disassembled fibrils are integrated again to form a dense morphology for pore walls after freeze-drying. While floc collagen has already a low disassembly degree, the alkali treatment could not be able to separate the fibrils, this remains as micropores in pore walls after freeze-drying. Both porous composites are significant in bone tissue engineering or regeneration. MTT test results showed the two composites had good cytocompatibility, and sponge collagen/TCP composite was somewhat better than floc collagen/TCP composite, which could result from that micropores derived roughness in pore walls of sponge collagen/TCP composite is suitable for cell growth.

Methyl 4-mercaptobutyrimidate as a cleavable crosslinking reagent and its application to the Escherichia coli 30S ribosome

Biochemistry ◽  
1973 ◽  
Vol 12 (17) ◽  
pp. 3266-3273 ◽  
Author(s):  
Robert R. Traut ◽  
Alex Bollen ◽  
Tung-Tien Sun ◽  
John W. B. Hershey ◽  
John Sundberg ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Crosslinking Reagent
Sign in / Sign up

Export Citation Format

Share Document