scholarly journals Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment

2003 ◽  
Vol 67A (2) ◽  
pp. 531-537 ◽  
Author(s):  
Kyung Mi Woo ◽  
Victor J. Chen ◽  
Peter X. Ma
Keyword(s):  
Protein Adsorption ◽  
Cell Attachment

Controlled radical polymerization of hydrophilic and zwitterionic brush-like polymers from silk fibroin surfaces

2020 ◽  
Vol 8 (45) ◽  
pp. 10392-10406
Author(s):  
Danielle L. Heichel ◽  
Ngoc Chau H. Vy ◽  
Shawn P. Ward ◽  
Douglas H. Adamson ◽  
Kelly A. Burke
Keyword(s):  
Protein Adsorption ◽  
Radical Polymerization ◽  
Silk Fibroin ◽  
Cell Attachment ◽  
Controlled Radical Polymerization

Silk fibroin films were modified with zwitterionic and hydrophilic brush-like polymers via surface-initiated ATRP, resulting in surfaces that reduced protein adsorption and cell attachment.

Tailoring Polymer Surfaces for Controlled Cell Behavior

1998 ◽  
Vol 530 ◽  
Author(s):  
A.M. Mayes ◽  
D.J. Irvine ◽  
L.G. Griffith
Keyword(s):  
Protein Adsorption ◽  
Surface Segregation ◽  
Cell Attachment ◽  
Model Systems ◽  
Protein Resistance ◽  
Small Proteins ◽  
Novel Approach ◽  
Poly Ethylene ◽  
Comb Copolymer ◽  
Adhesion Ligand

AbstractAn ideal surface for many biomaterials applications would resist nonspecific protein adsorption while at the same time providing a means for specifically signaling cells to guide survival, growth, migration, and differentiation. This work was directed towards the investigation of model systems and clinically-applicable materials which provide both of these surface requirements. Model systems were prepared by chemically grafting end-functionalized star poly(ethylene oxide) (PEO) to surfaces. The end-grafted polymers provide a means for cell-signaling through coupling of peptides to the free chain ends. Protein adsorption on star vs. linear grafted layers was compared. To further understand these results, neutron reflectivity studies were carried out in situ for solvated PEO surfaces to determine the concentration profiles of the swollen grafted layers. Surprisingly, grafted PEO layers which resist protein adsorption have low concentrations of polymer segments throughout the swollen layer. We find that dense star architectures which might be expected to impart improved protein resistance in fact allow small proteins to adsorb. For clinical materials, a novel approach to the surface modification of poly(lactide) (PLA) has been taken by surface segregating a comb copolymer containing a PLA backbone and poly(ethylene glycol) teeth. The ends of the teeth provide sites for surface tethering of peptide ligands. Comb surfaces without tethered ligands are cell adhesion resistant, indicating strong protein adsorption resistance. By then incorporating an adhesion ligand, modulation of cell morphology on comb surfaces has been demonstrated. Finally, the surface segregation of the comb to the surface of PLA was shown via cell attachment assays and XPS measurements.

scholarly journals Bacterial Adhesion at Synthetic Surfaces

1999 ◽  
Vol 65 (11) ◽  
pp. 4995-5002 ◽  
Author(s):  
D. Cunliffe ◽  
C. A. Smart ◽  
C. Alexander ◽  
E. N. Vulfson
Keyword(s):  
Molecular Weight ◽  
Protein Adsorption ◽  
Bacterial Adhesion ◽  
Cell Attachment ◽  
Systematic Investigation ◽  
Bacterial Attachment ◽  
E Coli ◽  
Glass Substrates ◽  
Cell Adsorption

ABSTRACT A systematic investigation into the effect of surface chemistry on bacterial adhesion was carried out. In particular, a number of physicochemical factors important in defining the surface at the molecular level were assessed for their effect on the adhesion ofListeria monocytogenes, Salmonella typhimurium,Staphylococcus aureus, and Escherichia coli. The primary experiments involved the grafting of groups varying in hydrophilicity, hydrophobicity, chain length, and chemical functionality onto glass substrates such that the surfaces were homogeneous and densely packed with functional groups. All of the surfaces were found to be chemically well defined, and their measured surface energies varied from 15 to 41 mJ · m−2. Protein adsorption experiments were performed with3H-labelled bovine serum albumin and cytochromec prior to bacterial attachment studies. Hydrophilic uncharged surfaces showed the greatest resistance to protein adsorption; however, our studies also showed that the effectiveness of poly(ethyleneoxide) (PEO) polymers was not simply a result of its hydrophilicity and molecular weight alone. The adsorption of the two proteins approximately correlated with short-term cell adhesion, and bacterial attachment for L. monocytogenes and E. coli also correlated with the chemistry of the underlying substrate. However, for S. aureus and S. typhimurium a different pattern of attachment occurred, suggesting a dissimilar mechanism of cell attachment, although high-molecular-weight PEO was still the least-cell-adsorbing surface. The implications of this for in vivo attachment of cells suggest that hydrophilic passivating groups may be the best method for preventing cell adsorption to synthetic substrates provided they can be grafted uniformly and in sufficient density at the surface.

Phosphorylcholine-containing polyurethanes for the control of protein adsorption and cell attachment via photoimmobilized laminin oligopeptides

1999 ◽  
Vol 10 (9) ◽  
pp. 931-955 ◽  
Author(s):  
L. Ruiz ◽  
E. Fine ◽  
J. Vörös ◽  
S.A. Makohliso ◽  
D. Léonard ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Cell Attachment

scholarly journals Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies

Biomaterials ◽  
2007 ◽  
Vol 28 (5) ◽  
pp. 851-860 ◽  
Author(s):  
C WITTMER ◽  
J PHELPS ◽  
W SALTZMAN ◽  
P VANTASSEL
Keyword(s):  
Protein Adsorption ◽  
Cell Attachment ◽  
Multilayer Films
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