scholarly journals Detection of oral squamous cell carcinoma with ex vivo fluorescence confocal microscopy: Sensitivity and specificity compared to histopathology

2020 ◽  
Vol 13 (9) ◽  
Author(s):  
Veronika Shavlokhova ◽  
Christa Flechtenmacher ◽  
Sameena Sandhu ◽  
Maximilian Pilz ◽  
Michael Vollmer ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Sensitivity And Specificity ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Fluorescence Confocal Microscopy

scholarly journals Features of oral squamous cell carcinoma in ex vivo fluorescence confocal microscopy

2020 ◽  
Author(s):  
Veronika Shavlokhova ◽  
Christa Flechtenmacher ◽  
Sameena Sandhu ◽  
Michael Vollmer ◽  
Jürgen Hoffmann ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Fluorescence Confocal Microscopy

scholarly journals Deep Learning on Oral Squamous Cell Carcinoma Ex Vivo Fluorescent Confocal Microscopy Data: A Feasibility Study

2021 ◽  
Vol 10 (22) ◽  
pp. 5326
Author(s):  
Veronika Shavlokhova ◽  
Sameena Sandhu ◽  
Christa Flechtenmacher ◽  
Istvan Koveshazi ◽  
Florian Neumeier ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Diagnostic Methods ◽  
Automated Classification ◽  
Cancerous Tissue

Background: Ex vivo fluorescent confocal microscopy (FCM) is a novel and effective method for a fast-automatized histological tissue examination. In contrast, conventional diagnostic methods are primarily based on the skills of the histopathologist. In this study, we investigated the potential of convolutional neural networks (CNNs) for automatized classification of oral squamous cell carcinoma via ex vivo FCM imaging for the first time. Material and Methods: Tissue samples from 20 patients were collected, scanned with an ex vivo confocal microscope immediately after resection, and investigated histopathologically. A CNN architecture (MobileNet) was trained and tested for accuracy. Results: The model achieved a sensitivity of 0.47 and specificity of 0.96 in the automated classification of cancerous tissue in our study. Conclusion: In this preliminary work, we trained a CNN model on a limited number of ex vivo FCM images and obtained promising results in the automated classification of cancerous tissue. Further studies using large sample sizes are warranted to introduce this technology into clinics.

Thallium-201 SPECT predicts response to intensity-modulated radiation therapy for recurrent oral squamous cell carcinoma

2007 ◽  
Vol 46 (05) ◽  
pp. 169-174
Author(s):  
J. K. Lee ◽  
W. S. Liu ◽  
Y. S. Lin ◽  
J. T. Liu ◽  
L. P. Chang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Radiation Therapy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Sensitivity And Specificity ◽  
Squamous Cell ◽  
Photon Emission ◽  
Thallium 201 ◽  
Emission Computed Tomography ◽  
Cutoff Value

SummaryThe aim of this study was designed to investigate the usefulness of thallium-201 single photon emission computed tomography (SPECT) in predicting response of intensitymodulated radiation therapy (IMRT) for recurrent oral squamous cell carcinoma (OSCC). Patients, methods: Thirty-one patients with histologically proven recurrent OSCC were recruited. Before IMRT, all patients underwent SPECT at 15 min and 120 min after intravenous injection 111 MBq of thallous chloride (201Tl). Tumour uptake was measured with the semiquantitative early ratio (ER), delayed ratio (DR), and retention index (RI). The patients were classified into responder (complete response and partial response) and non-responder (stable disease and progressive disease) groups according to the tumour response evaluated by the Response Evaluation Criteria in Solid Tumors guidelines. Results: As comparing DR and RI between the two groups, both parameters were significantly higher in the responder group. When a DR of 1.75 was used as a cutoff value for the responders, the receiver operating characteristic (ROC) analyses revealed sensitivity and specificity of 79% and 75%, respectively. At a cutoff value for the responders of a RI of 78.5%, the ROC analyses showed sensitivity and specificity of 95% and 83%, respectively. Conclusion: Our results suggest that 201Tl-SPECT, as measured by the DR or RI has clinical potential in predicting response of IMRT for patients with recurrent OSCC. The ROC curve analyses further suggest that RI may be superior to DR in distinguishing responders from non-responders.

Proton HR-MAS MR spectroscopy of oral squamous cell carcinoma tissues: an ex vivo study to identify malignancy induced metabolic fingerprints

Metabolomics ◽  
2010 ◽  
Vol 7 (2) ◽  
pp. 278-288 ◽  
Author(s):  
Shatakshi Srivastava ◽  
Raja Roy ◽  
Vivek Gupta ◽  
Ashish Tiwari ◽  
Anand N. Srivastava ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Mr Spectroscopy ◽  
Ex Vivo ◽  
Ex Vivo Study

Ex vivo confocal microscopy features of cutaneous squamous cell carcinoma

2018 ◽  
Vol 11 (4) ◽  
pp. e201700318 ◽  
Author(s):  
Daniela Hartmann ◽  
Sebastian Krammer ◽  
Mario R. Bachmann ◽  
Leonie Mathemeier ◽  
Thomas Ruzicka ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Cutaneous Squamous Cell Carcinoma

scholarly journals Estimation of serum matrix metalloproteinases among patients of oral squamous cell carcinoma

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Nakhshab Choudhry ◽  
Sana Sarmad ◽  
Noor Ul Ain Waheed ◽  
Aamir Jamal Gondal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Matrix Metalloproteinases ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Sensitivity And Specificity ◽  
Squamous Cell ◽  
Venous Blood ◽  
Serum Levels ◽  
Control Group ◽  
Creative Commons

Objective: To estimate the serum levels of matrix metalloproteinases in oral squamous cell carcinoma patients and in healthy subjects. Methods: In this observational study, biopsy diagnosed oral squamous cell carcinoma patients (n= 38) were recruited from Mayo Hospital, Lahore during 2016 to 2017. Age and gender matched Controls (n= 38) were also included. Venous blood sample of each participant was drawn, serum separated and the levels of matrix metalloproteinases were measured by multiplex ELISA. Results: Serum levels of MMP-1, -8, -10, -12 and -13 in OSCC patients showed statistically significant increase as compared to control group (p < 0.01). The MMP-12 predicted the presence of OSCC with highest AUC of 0.836 (95% CI [0.733 to 0.911]) for sensitivity and specificity of 80% and 78.9%, respectively for a cut-off value of 16.13 pg/ml. Conclusions: MMP-12 has been found to have significant sensitivity and specificity to qualify as a diagnostic biomarker. How to cite this:Choudhry N, Sarmad S, Waheed NA, Gondal AJ. Estimation of serum matrix metalloproteinases among patients of oral squamous cell carcinoma. Pak J Med Sci. 2019;35(1):252-256. doi: https://doi.org/doi.org/10.12669/pjms.35.1.68 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Increased Phosphatidylserine on Blood Cells in Oral Squamous Cell Carcinoma

2019 ◽  
Vol 98 (7) ◽  
pp. 763-771 ◽  
Author(s):  
Y. Liu ◽  
B. Li ◽  
T.L. Hu ◽  
T. Li ◽  
Y. Zhang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Confocal Microscopy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Blood Cells ◽  
Therapeutic Strategy ◽  
Stage Iii ◽  
Healthy Controls ◽  
Rapid Coagulation

The specific function of phosphatidylserine (PS) in the context of the development of a hypercoagulable state among individuals with oral squamous cell carcinoma (OSCC) is uncertain. The goal of this study was therefore to assess the exposure of PS on microparticles (MPs) as well as on endothelial and blood cells and to assess procoagulant activity (PCA) as a function of the stage of OSCC progression. We recruited patients with OSCC ( n = 63) as well as healthy controls ( n = 26) to participate in this study. PS exposure was then assessed via confocal microscopy and flow cytometry, revealing that patients with stage III/IV OSCC exhibited higher frequencies of PS-exposing blood cells, MPs, and serum-cultured endothelial cells (ECs) than did patients with stage I/II OSCC or healthy controls. When we conducted functional coagulation assays, we discovered that PS+blood cells, MPs, and serum-cultured ECs from patients with stage III/IV OSCC mediated more rapid coagulation and more substantial production of FXa, thrombin, and fibrin as compared with controls. When samples were treated with the PS antagonist lactadherin, this resulted in an 80% disruption of PCA. Strikingly, when pre- and postoperative samples were compared from patients with stage III/IV OSCC undergoing resective surgery, PCA was significantly reduced in the postoperative samples. After stimulating ECs with inflammatory cytokines, we found by confocal microscopy that they expose PS on their cell membranes, thus generating FVa and FXa binding sites and mediating the formation of fibrin. Together our findings provide evidence that PS+blood cells and MPs are important mediators of the development of a hypercoagulable and prothrombotic state among individuals afflicted by advanced-stage OSCC. As such, a PS blockade may be a viable therapeutic strategy for treating such patients.

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