Visualization photofragmentation‐induced rhodamine B release from gold nanorod delivery system by combination two‐photon luminescence imaging with correlation spectroscopy

2020 ◽  
Vol 13 (4) ◽  
Author(s):  
Shiqing Dong ◽  
Xiuqin Chen ◽  
Hongqin Yang ◽  
Xiaoqiong Tang ◽  
Jianling Chen ◽  
...  
Keyword(s):  
Delivery System ◽  
Rhodamine B ◽  
Correlation Spectroscopy ◽  
Gold Nanorod ◽  
Luminescence Imaging ◽  
Two Photon

Two-photon fluorescence excitation and related techniques in biological microscopy

2005 ◽  
Vol 38 (2) ◽  
pp. 97-166 ◽  
Author(s):  
Alberto Diaspro ◽  
Giuseppe Chirico ◽  
Maddalena Collini
Keyword(s):  
Optical Microscopy ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Basic Principles ◽  
Fluorescence Correlation ◽  
Two Photon ◽  
Photon Excitation ◽  
Biological Studies ◽  
Laser Sources ◽  
Saturation Effects

1. Introduction 982. Historical background of two-photon effects 992.1 2PE 1002.2 Harmonic generation 1002.3 Fluorescence correlation spectroscopy 1003. Basic principles of two-photon excitation of fluorescent molecules and implications for microscopy and spectroscopy 1013.1 General considerations 1013.2 Fluorescence intensity under the 2PE condition 1033.3 Optical consequences of 2PE 1043.4 Saturation effects in 2PE 1083.5 Fluorescence correlation spectroscopy 1093.5.1 Autocorrelation analysis 1103.5.2 Photon-counting histogram analysis 1124. Two-photon-excited probes 1155. Design considerations for a 2PE fluorescence microscope 1195.1 General aspects 1195.2 Descanned and non-descanned 2PE imaging 1215.3 Lens objectives and pulse broadening 1225.4 Laser sources 1255.5 Example of a practical realization 1276. Applications 1346.1 Biological applications of 2PE 1346.1.1 Brain images 1346.1.2 Applications on the kidney 1396.1.3 Mammalian embryos 1396.1.4 Applications to immuno-response 1416.1.5 Myocytes 1416.1.6 Retina 1426.1.7 DNA imaging 1436.1.8 FISH applications 1446.2 2PE imaging of single molecules 1446.3 FCS applications 1486.4 Signals from nonlinear interactions 1517. Conclusions 1538. Acknowledgements 1549. References 155This review is concerned with two-photon excited fluorescence microscopy (2PE) and related techniques, which are probably the most important advance in optical microscopy of biological specimens since the introduction of confocal imaging. The advent of 2PE on the scene allowed the design and performance of many unimaginable biological studies from the single cell to the tissue level, and even to whole animals, at a resolution ranging from the classical hundreds of nanometres to the single molecule size. Moreover, 2PE enabled long-term imaging of in vivo biological specimens, image generation from deeper tissue depth, and higher signal-to-noise images compared to wide-field and confocal schemes. However, due to the fact that up to this time 2PE can only be considered to be in its infancy, the advantages over other techniques are still being evaluated. Here, after a brief historical introduction, we focus on the basic principles of 2PE including fluorescence correlation spectroscopy. The major advantages and drawbacks of 2PE-based experimental approaches are discussed and compared to the conventional single-photon excitation cases. In particular we deal with the fluorescence brightness of most used dyes and proteins under 2PE conditions, on the optical consequences of 2PE, and the saturation effects in 2PE that mostly limit the fluorescence output. A complete section is devoted to the discussion of 2PE of fluorescent probes. We then offer a description of the central experimental issues, namely: choice of microscope objectives, two-photon excitable dyes and fluorescent proteins, choice of laser sources, and effect of the optics on 2PE sensitivity. An inevitably partial, but vast, overview of the applications and a large and up-to-date bibliography terminate the review. As a conclusive comment, we believe that 2PE and related techniques can be considered as a mainstay of the modern biophysical research milieu and a bright perspective in optical microscopy.

Influence of laser and material parameters on two photon absorption in Rhodamine B and Rhodamine 6G solutions in MeOH

Optik ◽  
2019 ◽  
Vol 183 ◽  
pp. 835-841 ◽  
Author(s):  
Rabia Ahmad ◽  
M. Shahid Rafique ◽  
Ali Ajami ◽  
Shazia Bashir ◽  
Wolfgang Husinsky ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Rhodamine 6G ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Material Parameters ◽  
Two Photon

Single Particle Studies on Two-Photon Photoluminescence of Gold Nanorod–Nanosphere Heterodimers

2016 ◽  
Vol 120 (21) ◽  
pp. 11621-11630 ◽  
Author(s):  
Monalisa Garai ◽  
Taishi Zhang ◽  
Nengyue Gao ◽  
Hai Zhu ◽  
Qing-Hua Xu
Keyword(s):  
Single Particle ◽  
Gold Nanorod ◽  
Two Photon

scholarly journals Two-photon absorption-induced photoacoustic imaging of Rhodamine B dyed polyethylene spheres using a femtosecond laser

2013 ◽  
Vol 21 (19) ◽  
pp. 22410 ◽  
Author(s):  
Gregor Langer ◽  
Klaus-Dieter Bouchal ◽  
Hubert Grün ◽  
Peter Burgholzer ◽  
Thomas Berer
Keyword(s):  
Femtosecond Laser ◽  
Rhodamine B ◽  
Photoacoustic Imaging ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon

Elucidation of the mechanism and energy barrier for anesthetic triggered membrane fusion in model membranes

2019 ◽  
Vol 97 (6) ◽  
pp. 474-482
Author(s):  
Trinh T. Nguyen ◽  
David T. Cramb
Keyword(s):  
Transition State ◽  
Membrane Fusion ◽  
Energy Barrier ◽  
Activation Barrier ◽  
Correlation Spectroscopy ◽  
Model Membranes ◽  
General Anesthetics ◽  
New Class ◽  
Two Photon ◽  
Photon Excitation

Membrane fusion is vital for cellular function and is generally mediated via fusogenic proteins and peptides. The mechanistic details and subsequently the transition state dynamics of membrane fusion will be dependent on the type of the fusogenic agent. We have previously established the potential of general anesthetics as a new class of fusion triggering agents in model membranes. We employed two-photon excitation fluorescence cross-correlation spectroscopy (TPE-FCCS) to report on vesicle association kinetics and steady-state fluorescence dequenching assays to monitor lipid mixing kinetics. Using halothane to trigger fusion in 110 nm diameter dioleoylphosphatidylcholine (DOPC) liposomes, we found that lipid rearrangement towards the formation of the fusion stalk was rate limiting. The activation barrier for halothane induced membrane fusion in 110 nm vesicles was found to be ∼40 kJ mol−1. We calculated the enthalpy and entropy of the transition state to be ∼40 kJ mol−1 and ∼180 J mol−1 K−1, respectively. We have found that the addition of halothane effectively lowers the energy barrier for membrane fusion in less curved vesicles largely due to entropic advantages.

LASER INDUCED THERMAL LENS EFFECT IN RHODAMINE B — SIGNATURE OF RESONANT TWO PHOTON ABSORPTION

1995 ◽  
Vol 09 (22) ◽  
pp. 1471-1477 ◽  
Author(s):  
C. V. BINDHU ◽  
S. S. HARILAL ◽  
RIJU C. ISSAC ◽  
GEETHA K. VARIER ◽  
V. P. N. NAMPOORI ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Laser Power ◽  
Photon Absorption ◽  
Thermal Lensing ◽  
Absorption Process ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Photon Process ◽  
Thermal Lens Effect ◽  
Made In

Measurement of thermal lensing signal as a function of laser power made in Rhodamine B solutions in methanol give clear evidence of two photon absorption process within certain concentration ranges when 488 nm Ar + laser beam is used as the pump source. Only one photon process is found to occur when 514 nm and 476 nm beams are used as the pump.

scholarly journals Stability of Positively Charged Nanoemulsion Formulation Containing Steroidal Drug for Effective Transdermal Application

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Stephanie Da Costa ◽  
Mahiran Basri ◽  
Norashikin Shamsudin ◽  
Hamidon Basri
Keyword(s):  
Particle Size ◽  
Delivery System ◽  
Correlation Spectroscopy ◽  
Good Stability ◽  
Ph Values ◽  
Transdermal Application ◽  
Oil In Water ◽  
Steroid Drugs ◽  
Positively Charged ◽  
Dermal Application

This paper emphasizes the formation of a positively charged nanoemulsion system for steroid drugs (hydrocortisone). It is believed that positively charged nanoemulsion provides more effective penetration of the skin. Therefore in our study we focused on the incorporation of phytosphingosine which serves as a positively charged cosurfactant in the nanoemulsion system. Negatively charged nanoemulsions were formulated mainly for comparison. Freshly prepared formulations were formed with particle size less than 300 nm and showed good stability over time. The oil-in-water nanoemulsion also showed good viscosity, conductivity, and pH values. From TEM micrograph, particle size showed consistent results with the measurement using photon correlation spectroscopy. It was concluded that both positively and negatively charged nanoemulsions showed good stability and have great potential in transdermal delivery system. Though, further investigation of the drug release and drug penetration of both positively and negatively charged nanoemulsions will be studied to further prove the efficacy of nanoemulsion with hydrocortisone as a delivery system for dermal application.

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