Optical sectioning and high resolution visualization of trabecular meshwork using Bessel beam assisted light sheet fluorescence microscopy

2019 ◽  
Vol 12 (12) ◽  
Author(s):  
C. S. Suchand Sandeep ◽  
Sreelatha Sarangapani ◽  
Xun J. J. Hong ◽  
Tin Aung ◽  
Mani Baskaran ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Trabecular Meshwork ◽  
Bessel Beam ◽  
Light Sheet ◽  
Optical Sectioning ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Fast multi-directional DSLM for confocal detection without striping artifacts

2020 ◽  
Author(s):  
Pietro Ricci ◽  
Giuseppe Sancataldo ◽  
Vladislav Gavryusev ◽  
Alessandra Franceschini ◽  
Marie Caroline Müllenbroich ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Light Sheet ◽  
Optical Sectioning ◽  
Single Plane ◽  
Image Recording ◽  
Optical Deflector ◽  
Scattering Effects ◽  
Photo Damage ◽  
Light Sheet Fluorescence Microscopy ◽  
Confocal Detection

AbstractIn recent years light-sheet fluorescence microscopy (LSFM) has become a cornerstone technology for neuroscience, improving quality and capabilities of 3D imaging. By selectively illuminating a single plane, it provides intrinsic optical sectioning and fast image recording, while minimizing out of focus fluorescence background, sample photo-damage and photo-bleaching. However, images acquired with LSFM are often affected by light absorption or scattering effects, leading to un-even illumination and striping artifacts. In this work we present an optical solution to this problem, via fast multi-directional illumination of the sample, based on an acousto-optical deflector (AOD). We demonstrate that this pivoting system is compatible with confocal detection in digital scanned laser light-sheet fluorescence microscopy (DSLM) by using a pivoted elliptical-Gaussian beam. We tested its performance by acquiring signals emitted by specific fluorophores in several mouse brain areas, comparing the pivoting beam illumination and a traditional static one, measuring the point spread function response and quantifying the striping reduction. We observed real-time shadow suppression, while preserving the advantages of confocal detection for image contrast.

Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging

2018 ◽  
Vol 16 (11) ◽  
pp. 111801 ◽  
Author(s):  
Suhui Deng Suhui Deng ◽  
Yiping Xiao Yiping Xiao ◽  
Jie Hu Jie Hu ◽  
Jianfang Chen Jianfang Chen ◽  
Yuhao Wang Yuhao Wang ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Bessel Beam ◽  
Light Sheet ◽  
Sidelobe Suppression ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Improving axial resolution of Bessel beam light-sheet fluorescence microscopy by photobleaching imprinting

2020 ◽  
Vol 28 (7) ◽  
pp. 9464 ◽  
Author(s):  
Bo Xiong ◽  
Xiaofei Han ◽  
Jiamin Wu ◽  
Hao Xie ◽  
Qionghai Dai
Keyword(s):  
Fluorescence Microscopy ◽  
Bessel Beam ◽  
Light Sheet ◽  
Axial Resolution ◽  
Light Sheet Fluorescence Microscopy

Light Sheet Fluorescence Microscopy of the Trabecular Meshwork in Rodent Eyes

2020 ◽  
Author(s):  
C. S. Suchand Sandeep ◽  
Veluchamy Amutha Barathi ◽  
Tin Aung ◽  
Mani Baskaran ◽  
Murukeshan Vadakke Matham
Keyword(s):  
Fluorescence Microscopy ◽  
Trabecular Meshwork ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy

scholarly journals Single-objective high-resolution light sheet fluorescence microscopy for standard biological sample geometries

2021 ◽  
Author(s):  
Knut Drescher ◽  
Stoyan Yordanov ◽  
Konstantin Neuhaus ◽  
Raimo Hartmann ◽  
Francisco Diaz-Pascual ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Biological Sample ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy ◽  
Single Objective

High resolution 3D imaging of primary and secondary tumor spheroids using multicolor multi-angle Light Sheet Fluorescence Microscopy (LSFM)

2019 ◽  
Author(s):  
Stylianos E. Psycharakis ◽  
Evangelos Liapis ◽  
Athanasios Zacharopoulos ◽  
Mariam-Eleni Oraiopoulou ◽  
Chrysoula Aivalioti ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
3D Imaging ◽  
Light Sheet ◽  
Tumor Spheroids ◽  
Secondary Tumor ◽  
Light Sheet Fluorescence Microscopy
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