scholarly journals Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

2019 ◽  
Vol 12 (5) ◽  
Author(s):  
Anton Y. Sdobnov ◽  
Maxim E. Darvin ◽  
Johannes Schleusener ◽  
Jürgen Lademann ◽  
Valery V. Tuchin
Keyword(s):  
Quantitative Analysis ◽  
Bound Water ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Optical Clearing ◽  
Confocal Raman ◽  
Hydrogen Bound

Confocal Raman microscopy combined with optical clearing for identification of inks in multicolored tattooed skinin vivo

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4990-4999 ◽  
Author(s):  
Maxim E. Darvin ◽  
Johannes Schleusener ◽  
Franziska Parenz ◽  
Olaf Seidel ◽  
Christoph Krafft ◽  
...  
Keyword(s):  
Human Skin ◽  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Optical Clearing ◽  
Confocal Raman

Tattoo ink pigments were non-invasively identified in multicolored tattooed human skinin vivousing optical clearing enhanced confocal Raman microscopy.

Confocal Raman microscopy supported by optical clearing treatment of the skin—influence on collagen hydration

2017 ◽  
Vol 50 (28) ◽  
pp. 285401 ◽  
Author(s):  
Anton Yu Sdobnov ◽  
Valery V Tuchin ◽  
Juergen Lademann ◽  
Maxim E Darvin
Keyword(s):  
Raman Microscopy ◽  
Confocal Raman Microscopy ◽  
Optical Clearing ◽  
Confocal Raman

Depth profiles of hydrogen bound water molecule types and their relation to lipid and protein interaction in the human stratum corneum in vivo

The Analyst ◽  
2016 ◽  
Vol 141 (22) ◽  
pp. 6329-6337 ◽  
Author(s):  
ChunSik Choe ◽  
Jürgen Lademann ◽  
Maxim E. Darvin
Keyword(s):  
Hydrogen Bonding ◽  
Water Molecule ◽  
Gaussian Function ◽  
Bound Water ◽  
Confocal Raman Microscopy ◽  
Depth Profiles ◽  
Confocal Raman ◽  
Hydrogen Bound ◽  
Water Profile

Confocal Raman microscopy has been used to measure the depth-dependent profiles of hydrogen bound water molecule types in human SCin vivousing the Gaussian function-based deconvolution procedure of the HWN region. The hydrogen bonding state of the water profile in the SC has been determined.

Single Layer Graphene for Estimation of Axial Spatial Resolution in Confocal Raman Microscopy Depth Profiling

2018 ◽  
Vol 91 (1) ◽  
pp. 1049-1055 ◽  
Author(s):  
Carol Korzeniewski ◽  
Jay P. Kitt ◽  
Saheed Bukola ◽  
Stephen E. Creager ◽  
Shelley D. Minteer ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Depth Profiling ◽  
Single Layer ◽  
Raman Microscopy ◽  
Single Layer Graphene ◽  
Confocal Raman Microscopy ◽  
Layer Graphene ◽  
Confocal Raman

scholarly journals Real-Time Drug Release Imaging From Nanocomposite Drug and Polymer Coatings

2008 ◽  
Vol 2 (2) ◽  
Author(s):  
Jinping Dong ◽  
Greg Haugstad ◽  
Chris Frethem ◽  
John Foley ◽  
Bob Hoerr ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Polymer Coatings ◽  
Raman Microscopy ◽  
Prolonged Release ◽  
Confocal Raman Microscopy ◽  
Release Process ◽  
Force Microscopy ◽  
Confocal Raman ◽  
Model Drug

The ElectroNanospray process (Nanocopoeia, Inc) transforms drugs and polymers into many nanoscale material states including powders, liquids, encapsulated particles, and coatings. This allows application of polymers and drugs to the surface of medical devices such as coronary stents in a single-stage process. A model drug delivery system consisting of a polymer matrix (arborescent polyisobutylene-polystyrene, or arbIBS) and either dexamethasone or sirolimus was studied by various characterization techniques. Modification of ElectroNanospray process parameters resulted in surface coatings with rich morphologies that are revealed by SEM, Atomic Force Microscopy (AFM) and Confocal Raman Microscopy were employed to monitor the drug release process in situ, through which the mechanism of the drug-eluting process may be proposed. A Confocal Raman microscope fitted with underwater objective was used to image arbIBS∕drug films incubated in phosphate-buffered saline over 12h and at various film depths. Drug migrated from more concentrated areas into the surrounding polymer and toward the surface, beginning as early as 5min after placing the sample in buffer and continuing throughout the 12h period. High drug levels remained in the more concentrated areas at the end of incubation, suggesting the potential for prolonged release. SEM and AFM images taken from samples post incubation showed the appearance of nanoscale pores ∼100nm in diameter in areas corresponding in size and distribution to the Confocal Raman planar image areas of increased drug concentration. Confocal Raman microscopy offers a powerful new technique for demonstrating real-time drug release from therapeutic medical device coatings.

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