The impact of cell fixation on coherent anti-stokes Raman scattering signal intensity in neuronal and glial cell lines

2018 ◽  
Vol 12 (1) ◽  
pp. e201800203 ◽  
Author(s):  
Svitlana M. Levchenko ◽  
Xiao Peng ◽  
Liwei Liu ◽  
Junle Qu
Keyword(s):  
Raman Scattering ◽  
Glial Cell ◽  
Cell Lines ◽  
Signal Intensity ◽  
Anti Stokes ◽  
The Impact ◽  
Scattering Signal ◽  
Cell Fixation

Time-Domain Coherent Anti-Stokes Raman Scattering Signal Detection for Terahertz Vibrational Spectroscopy Using Chirped Femtosecond Pulses

2010 ◽  
Vol 3 (7) ◽  
pp. 072401 ◽  
Author(s):  
Masahiko Tani ◽  
Toshiyuki Koizumi ◽  
Hisashi Sumikura ◽  
Mariko Yamaguchi ◽  
Kohji Yamamoto ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Signal Detection ◽  
Vibrational Spectroscopy ◽  
Time Domain ◽  
Femtosecond Pulses ◽  
Anti Stokes ◽  
Scattering Signal

scholarly journals Optimal coherent control of coherent anti-Stokes Raman scattering: Signal enhancement and background elimination

2012 ◽  
Vol 136 (14) ◽  
pp. 144114 ◽  
Author(s):  
Fang Gao ◽  
Feng Shuang ◽  
Junhui Shi ◽  
Herschel Rabitz ◽  
Haifeng Wang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Coherent Control ◽  
Signal Enhancement ◽  
Background Elimination ◽  
Anti Stokes ◽  
Scattering Signal

scholarly journals A Preliminary Investigation into the Impact of a Pesticide Combination on Human Neuronal and Glial Cell Lines In Vitro

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e42768 ◽  
Author(s):  
Michael D. Coleman ◽  
John D. O'Neil ◽  
Elizabeth K. Woehrling ◽  
Oscar Bate Akide Ndunge ◽  
Eric J. Hill ◽  
...  
Keyword(s):  
Glial Cell ◽  
Cell Lines ◽  
Preliminary Investigation ◽  
The Impact

scholarly journals PC12 and THP-1 Cell Lines as Neuronal and Microglia Model in Neurobiological Research

2021 ◽  
Vol 11 (9) ◽  
pp. 3729
Author(s):  
Katarzyna Balon ◽  
Benita Wiatrak
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Cell Lines ◽  
Inflammatory Factor ◽  
Research Model ◽  
Differentiated Cells ◽  
Undifferentiated Cells ◽  
Primary Cell Lines ◽  
Neurobiological Research ◽  
The Impact

Models based on cell cultures have become a useful tool in modern scientific research. Since primary cell lines are difficult to obtain and handle, neoplasm-derived lines like PC12 and THP-1 offer a cheap and flexible solution for neurobiological studies but require prior differentiation to serve as a neuronal or microglia model. PC12 cells constitute a suitable research model only after differentiation by incubation with nerve growth factor (NGF) and THP-1 cells after administering a differentiation factor such as phorbol 12-myristate-13-acetate (PMA). Still, quite often, studies are performed on these cancer cells without differentiation. The study aimed to assess the impact of PC12 or THP-1 cell differentiation on sensitivity to harmful factors such as Aβ25-35 (0.001–5 µM) (considered as one of the major detrimental factors in the pathophysiology of Alzheimer’s disease) or lipopolysaccharide (1–100 µM) (LPS; a pro-inflammatory factor of bacterial origin). Results showed that in most of the tests performed, the response of PC12 and THP-1 cells induced to differentiation varied significantly from the effect in undifferentiated cells. In general, differentiated cells showed greater sensitivity to harmful factors in terms of metabolic activity and DNA damage, while in the case of the free radicals, the results were heterogeneous. Obtained data emphasize the importance of proper differentiation of cell lines of neoplastic origin in neurobiological research and standardization of cell culture handling protocols to ensure reliable results.

scholarly journals Selective Imaging of Microplastic and Organic Particles in Flow by Multimodal Coherent Anti-Stokes Raman Scattering and Two-Photon Excited Autofluorescence Analysis

2021 ◽  
Vol 93 (12) ◽  
pp. 5234-5240
Author(s):  
Tomoko Takahashi ◽  
Krzysztof Pawel Herdzik ◽  
Konstantinos Nikolaos Bourdakos ◽  
James Arthur Read ◽  
Sumeet Mahajan
Keyword(s):  
Raman Scattering ◽  
Two Photon ◽  
Organic Particles ◽  
Anti Stokes
Sign in / Sign up

Export Citation Format

Share Document