scholarly journals Holographic imaging of unlabelled sperm cells for semen analysis: a review

2014 ◽  
Vol 8 (10) ◽  
pp. 779-789 ◽  
Author(s):  
Giuseppe Di Caprio ◽  
Maria Antonietta Ferrara ◽  
Lisa Miccio ◽  
Francesco Merola ◽  
Pasquale Memmolo ◽  
...  
Keyword(s):  
Semen Analysis ◽  
Sperm Cells ◽  
Holographic Imaging

scholarly journals Unlabeled Semen Analysis by Means of the Holographic Imaging

10.5772/67552 ◽  
2017 ◽  
Author(s):  
Giuseppe Coppola ◽  
Maria Antonietta Ferrara ◽  
Giuseppe Di Caprio ◽  
Gianfranco Coppola ◽  
Brian Dale
Keyword(s):  
Semen Analysis ◽  
Holographic Imaging

scholarly journals Effect of Tiger Nut Meal on Some Sex Hormones and Sperm Cells in Androgen-induced Benign Prostate Hyperplasia in Adult Male Wistar Rats

2020 ◽  
pp. 74-82
Author(s):  
D. I. Izunwanne ◽  
J. N. Egwurugwu ◽  
C. L. Emegano
Keyword(s):  
Benign Prostate Hyperplasia ◽  
Semen Analysis ◽  
Reproductive Function ◽  
Reproductive Hormones ◽  
Male Rats ◽  
Estradiol Valerate ◽  
Sperm Cells ◽  
Prostate Hyperplasia ◽  
Ameliorative Effect ◽  
Benign Prostate

Introduction: It is generally believed that Cyperus esculentus (tiger nut) has some fertility boosting effects. However, scientific validation of some of the fertility boosting potentials as well as the ameliorative effect of tiger nut on Benign Prostate Hyperplasia (BPH) is lacking. Objective: The aim of the project is to determine the effect of tiger nuts in reproductive function in rats induced with Benign Prostate Hyperplasia (BPH). Methods: A total of sixty (60) male rats weighing between 160 – 200 g were used in this study. They were divided into six groups of ten rats per group. Benign prostate hyperplasia was induced in three groups of the rats (as stated in methodology) with 30 mg/kg sub-cutaneous injections of hormones containing dihydrotestosterone (DHT) and estradiol valerate dissolved in olive oil in the ratio of 10:1 (three times in a week, one day interval). Administration of tiger nut meal commenced immediately and lasted for two months. At the end of administration, assay of reproductive hormones (FSH, LH and Testosterone) was done. Comprehensive semen analysis was also done including (count, motility & morphology). Results: The study showed that the induction of BPH resulted in a very significant reduction of FSH while the administration of the tiger nut meal did not show any significant effect on FSH (P 0.05). The same trend was also observed on the effect of the tiger nut meal on LH level. For testosterone, it was observed that after the initial decrease as a result of the induction of infertility, 20% tiger nut meal administration significantly increased the testosterone level to 2.10±0.06 ng/ml from 0.30±0.01 ng/ml in the infertility-induced group. The effect of tiger nut meal on semen analyses namely, normal sperm cells and sperm motility showed a significant increase (P<0.05) following the induction of infertility. The effect of the tiger nut meal on sperm morphological toxicities were also examined. Sperm abnormalities like sperm cells with twisted tails were examined. Conclusion: The administration of tiger nut significantly ameliorated the abnormalities and thus, restored the morphology of the sperm cells such that it can enhance fertility.

Semen Analysis Profile on Varicocele Patients at Dr. Ramelan Navy Hospital Surabaya during Period 2015

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
TIFFANY WONGSODIHARJO
Keyword(s):  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Semen Analysis ◽  
Secondary Data ◽  
Married Couples ◽  
Sperm Cells ◽  
Descriptive Research ◽  
Normal Limits

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>: Infertility has affected about 10–15 percent married couples. Almost half of infertility cases have been attributed to men with low sperm motility (asthenozoospermia), low sperm count (oligozoospermia) and abnormal sperm morphology (teratozoospermia). Sperm motility is one of the major determinants of male fertility and is required for successful fertilization. Semen analysis is a method to know the quality of semen. Semen analysis consists of macroscopic and microscopic examination of sperm cells, which are concentration, motility and morphology of sperm cells. </p><p><strong>Objective</strong>:<strong> </strong>This study aimed to obtain the semen analysis profile on varicocele patients at DR. Ramelan Navy Hospital Surabaya during period 2015.</p><strong>Method</strong>: This study used descriptive research design and quantitative method, by using the secondary data were collected from medical records during January – December 2015. <strong>Result</strong>: In this study the semen analysis of 36 patients with varicocele, 3 patients (8,33%) with oligozoospermia, 10 patients (27,78%) with asthenozoospermia, 20 patients (55,55%) with oligoasthenozoospermia, 3 patients (8,33%) with extreme oligoasthenozoospermia. <strong>Conclusion</strong>: This study has concluded that all varicocele patients have abnormalities in semen analysis (none of the sperm quality results within normal limits).

Metabolic activity of sperm cells: correlation with sperm cell concentration, viability and motility in the rabbit

Zygote ◽  
2016 ◽  
Vol 24 (5) ◽  
pp. 707-713 ◽  
Author(s):  
Maria Sabés-Alsina ◽  
Núria Planell ◽  
Sílvia Gil ◽  
Oriol Tallo-Parra ◽  
Maria José Maya-Soriano ◽  
...  
Keyword(s):  
Metabolic Rate ◽  
Metabolic Activity ◽  
Semen Quality ◽  
Semen Analysis ◽  
Rank Correlation ◽  
Semen Parameters ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Spearman Rank Correlation Analysis ◽  
Status Information

SummaryThe resazurin reduction test (RRT) is a useful technique to assess the metabolic rate of sperm cells. RRT depends on the ability of metabolically active cells to reduce the non-fluorescent dye resazurin to the fluorescent resorufin. The aim of this study was to develop a vital fluorometric method to evaluate metabolic activity of rabbit sperm cells. Twenty-five rabbit males were included in the study. Viability and morphology, motility and metabolic activity were evaluated using an eosin-nigrosin staining, a computer-assisted semen analysis (CASA) and the RRT, respectively. Spearman rank correlation analysis was used to determine the correlation between RRT and semen parameters. After evaluation, a concentration of 10 × 106 sperm cells/ml was selected for further experiments with RRT. No significant correlation was found between the RRT results and the motility parameters. However, after RRT a significant positive correlation between relative fluorescence units and the percentage of alive spermatozoa (r = 0.62; P = 0.001) and a negative one with the percentage of sperm cells with acrosomic abnormalities (r = −0.45; P < 0.05) were detected.The vital assessment of metabolic rate of sperm cells by RRT could provide more information about semen quality than other routine semen analysis, correlating with sperm viability and acrosome status information.

Ultrastructure of Testicular Spermatozoon of the Fish Oreochromis Niloticus

1988 ◽  
Vol 46 ◽  
pp. 278-279
Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis
Keyword(s):  
Electron Microscope ◽  
Oreochromis Niloticus ◽  
Osmium Tetroxide ◽  
Sperm Cells ◽  
Adult Fish ◽  
Testicular Sperm ◽  
Thin Sections ◽  
Important Fish ◽  
Testicular Spermatozoon ◽  
Diamond Knife

Tilapia, Oreochromis niloticus, is an economically important fish in Saudi Arabia. Elucidation of reproductive biology of this species is necessary for successful breeding program. In this paper we describe fine structure of testicular sperm cells in O, niloticus.Testes from young adult fish were fixed in gluteraldehyde (2%) and osmium tetroxide (1%), both in cacodyl ate buffer. Specimens were processed in the conventional way for electron microscopy and thin sections of tissues (obtained by cutting the blocks with a diamond knife) were stained by ura- nyl acetate and lead citrate. These were examined in a Carl Zeiss electron microscope operated at 40 kV to 60 kV. Sperm cells were obtained from testes by squeezing them in cacodyl ate buffer. They were fixed in gluteraldehyde (2%) in the same buffer, air dried, gold coated and then examined in a Philips scanning electron microscope (SEM) operated at 25kV.The spermatozoon of O. niloticus is consisting of head, midpiece and tail (Fig. 1).

Performance and applications of the holography electron microscope

1993 ◽  
Vol 51 ◽  
pp. 1078-1079
Author(s):  
Akira Tonomura
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Phase Measurement ◽  
Electron Holography ◽  
Imaging Method ◽  
High Contrast ◽  
Magnetic Lens ◽  
High Brightness ◽  
Holographic Imaging ◽  
Dynamic Observation

Electron holography is a two-step imaging method. However, the ultimate performance of holographic imaging is mainly determined by the brightness of the electron beam used in the hologram-formation process. In our 350kV holography electron microscope (see Fig. 1), the decrease in the inherently high brightness of field-emitted electrons is minimized by superposing a magnetic lens in the gun, for a resulting value of 2 × 109 A/cm2 sr. This high brightness has lead to the following distinguished features. The minimum spacing (d) of carrier fringes is d = 0.09 Å, thus allowing a reconstructed image with a resolution, at least in principle, as high as 3d=0.3 Å. The precision in phase measurement can be as high as 2π/100, since the position of fringes can be known precisely from a high-contrast hologram formed under highly collimated illumination. Dynamic observation becomes possible because the current density is high.

PATTERN OF 17-KETOSTEROID URINARY EXCRETION IN INFERTILE MEN

1963 ◽  
Vol 42 (1) ◽  
pp. 97-100
Author(s):  
Mirjam Furuhjelm ◽  
Birgit Johnson ◽  
C.-G. Lagergren
Keyword(s):  
Urinary Excretion ◽  
Sperm Cells ◽  
Infertile Men ◽  
Abnormal Cells

ABSTRACT The pattern of the 17-ketosteroid excretion determined according to Johnsen (1956) in a group of 33 sterile men was compared with that in another group of 14 men with proved fertility, and no difference was found. No correlation between 17-ketosteroid excretion and the concentration of sperm cells or the percentage of abnormal cells could be detected. The results are discussed.

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