Depth-sensitive Raman spectroscopy combined with optical coherence tomography for layered tissue analysis

2013 ◽  
Vol 7 (1-2) ◽  
pp. 77-85 ◽  
Author(s):  
Khan M. Khan ◽  
Hemant Krishna ◽  
Shovan K. Majumder ◽  
K. Divakar Rao ◽  
Pradeep K. Gupta
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Tissue Analysis

scholarly journals Improvement of tissue analysis and classification using optical coherence tomography combined with Raman spectroscopy

2015 ◽  
Vol 08 (04) ◽  
pp. 1550006 ◽  
Author(s):  
Chih-Hao Liu ◽  
Ji Qi ◽  
Jing Lu ◽  
Shang Wang ◽  
Chen Wu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Real Time ◽  
Prediction Accuracy ◽  
Tissue Characterization ◽  
Optical Biopsy ◽  
Support Vector ◽  
Optical Coherence ◽  
Tissue Analysis ◽  
Real Time Imaging

Optical coherence tomography (OCT) provides significant advantages of high resolution (approaching the histopathology level) real-time imaging of tissues without use of contrast agents. Based on these advantages, the microstructural features of tumors can be visualized and detected intra-operatively. However, it is still not clinically accepted for tumor margin delineation due to poor specificity and accuracy. In contrast, Raman spectroscopy (RS) can obtain tissue information at the molecular level, but does not provide real-time imaging capability. Therefore, combining OCT and RS could provide synergy. To this end, we present a tissue analysis and classification method using both the slope of OCT intensity signal vs depth and the principle components from the RS spectrum as the indicators for tissue characterization. The goal of this study was to understand prediction accuracy of OCT and combined OCT/RS method for classification of optically similar tissues and organs. Our pilot experiments were performed on mouse kidneys, livers, and small intestines (SIs). The prediction accuracy with five-fold cross validation of the method has been evaluated by the support vector machine (SVM) method. The results demonstrate that tissue characterization based on the OCT/RS method was superior compared to using OCT structural information alone. This combined OCT/RS method is potentially useful as a noninvasive optical biopsy technique for rapid and automatic tissue characterization during surgery.

scholarly journals Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1445-1456 ◽  
Author(s):  
Fabian Placzek ◽  
Eliana Cordero Bautista ◽  
Simon Kretschmer ◽  
Lara M. Wurster ◽  
Florian Knorr ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Imaging System ◽  
Ex Vivo ◽  
Large Field ◽  
Optical Coherence ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Muscle Invasive

Characterization of bladder biopsies, using a combined fiber optic probe-based optical coherence tomography and Raman spectroscopy imaging system that allows a large field-of-view imaging and detection and grading of cancerous bladder lesions.

Biophotonic sensors on a silicon chip for Raman spectroscopy and optical coherence tomography

2012 ◽  
Author(s):  
Markus Pollnau ◽  
Nur Ismail ◽  
B. Imran Akca ◽  
Kerstin Wörhoff ◽  
René M. de Ridder
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Optical Coherence

scholarly journals Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue

2015 ◽  
Vol 6 (6) ◽  
pp. 2022 ◽  
Author(s):  
Jason R. Maher ◽  
Oranat Chuchuen ◽  
Marcus H. Henderson ◽  
Sanghoon Kim ◽  
Matthew T. Rinehart ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Confocal Raman Spectroscopy ◽  
Confocal Raman ◽  
Analyte Detection
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