Digital holographic microtomography for high-resolution refractive index mapping of live cells

2012 ◽  
Vol 6 (5) ◽  
pp. 416-424 ◽  
Author(s):  
Jing-Wei Su ◽  
Wei-Chen Hsu ◽  
Cheng-Ying Chou ◽  
Chen-Hao Chang ◽  
Kung-Bin Sung
Keyword(s):  
Refractive Index ◽  
High Resolution ◽  
Live Cells ◽  
Index Mapping

Scanning Ion Conductance Microscopy for High-Resolution Topography of Soft Samples Including Live Cells

2011 ◽  
Vol 17 (S2) ◽  
pp. 236-237
Author(s):  
G De Filippi ◽  
C Moore
Keyword(s):  
High Resolution ◽  
Live Cells ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Soft Samples

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

scholarly journals Label-Free Digital Holo-tomographic Microscopy Reveals Virus-Induced Cytopathic Effects in Live Cells

mSphere ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Artur Yakimovich ◽  
Robert Witte ◽  
Vardan Andriasyan ◽  
Fanny Georgi ◽  
Urs F. Greber
Keyword(s):  
Refractive Index ◽  
Vaccinia Virus ◽  
Virus Type ◽  
Live Cells ◽  
Refractive Index Gradient ◽  
Label Free ◽  
Virus Infections ◽  
Cytopathic Effects ◽  
Infected Cells ◽  
Index Gradient

ABSTRACTCytopathic effects (CPEs) are a hallmark of infections. CPEs are difficult to observe due to phototoxicity from classical light microscopy. We report distinct patterns of virus infections in live cells using digital holo-tomographic microscopy (DHTM). DHTM is label-free and records the phase shift of low-energy light passing through the specimen on a transparent surface with minimal perturbation. DHTM measures the refractive index (RI) and computes the refractive index gradient (RIG), unveiling optical heterogeneity in cells. We find that vaccinia virus (VACV), herpes simplex virus (HSV), and rhinovirus (RV) infections progressively and distinctly increased RIG. VACV infection, but not HSV and RV infections, induced oscillations of cell volume, while all three viruses altered cytoplasmic membrane dynamics and induced apoptotic features akin to those caused by the chemical compound staurosporine. In sum, we introduce DHTM for quantitative label-free microscopy in infection research and uncover virus type-specific changes and CPE in living cells with minimal interference.IMPORTANCEThis study introduces label-free digital holo-tomographic microscopy (DHTM) and refractive index gradient (RIG) measurements of live, virus-infected cells. We use DHTM to describe virus type-specific cytopathic effects, including cyclic volume changes of vaccinia virus infections, and cytoplasmic condensations in herpesvirus and rhinovirus infections, distinct from apoptotic cells. This work shows for the first time that DHTM is suitable to observe virus-infected cells and distinguishes virus type-specific signatures under noninvasive conditions. It provides a basis for future studies, where correlative fluorescence microscopy of cell and virus structures annotate distinct RIG values derived from DHTM.

scholarly journals High-resolution wetness index mapping: A useful tool for regional scale wetland management

2018 ◽  
Vol 48 ◽  
pp. 89-96 ◽  
Author(s):  
Thomas P. Higginbottom ◽  
C.D. Field ◽  
A.E. Rosenburgh ◽  
A. Wright ◽  
E. Symeonakis ◽  
...  
Keyword(s):  
High Resolution ◽  
Wetland Management ◽  
Regional Scale ◽  
Wetness Index ◽  
Index Mapping

Preparation of Patternable High Resolution and High Refractive Index Materials for AR/VR

2020 ◽  
pp. 286
Author(s):  
Jiro Hikida ◽  
Hiroki Chisaka ◽  
Yoichiro Ijima ◽  
Yuehchun Liao ◽  
Kenri Konno ◽  
...  
Keyword(s):  
Refractive Index ◽  
High Resolution ◽  
High Refractive Index

Microwave Photonic Interrogation of a High-Speed and High-Resolution Multipoint Refractive Index Sensor

2021 ◽  
pp. 1-1
Author(s):  
Guangying Wang ◽  
Baoliang Liao ◽  
Yuan Cao ◽  
Tuan Guo ◽  
Xinhuan Feng ◽  
...  
Keyword(s):  
Refractive Index ◽  
High Resolution ◽  
High Speed ◽  
Refractive Index Sensor ◽  
Microwave Photonic

Enhancement of Differential Interference Contrast Images of Live Cells Obtained With Digital Cameras

1999 ◽  
Vol 5 (S2) ◽  
pp. 1112-1113
Author(s):  
M.V. Parthasarathy
Keyword(s):  
High Resolution ◽  
Contrast Enhancement ◽  
Dynamic Range ◽  
Ccd Camera ◽  
Video Camera ◽  
Live Cells ◽  
Photographic Emulsion ◽  
Digital Cameras ◽  
Structural Detail ◽  
Ccd Cameras

The usefulness of Differential Contrast Interference (DIC) light microscopy for observing fine details within transparent specimens is well known. However, when viewed by the eye or by recording with photographic emulsion, fine structural detail at the limit of resolution is often not visible because of lack of contrast. To overcome this problem, electronic contrast enhancement capabilities of video cameras have been used to enhance structural details that would otherwise be invisible. The technique, commonly referred to as VE-DIC (Video Enhanced DIC), uses first analog contrast enhancement of the image with a video camera followed by a real-time digital image processor to further enhance the image with. We are exploring the feasibility of achieving fine structural detail of live cells by directly acquiring digital images of them with a high resolution CCD camera.High resolution cooled slow-scan 12-bit CCD cameras are well suited for DIC microscopy because of their greater dynamic range than video CCD cameras that are normally 8-bits or lower.

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