In vivo skin optical clearing by glycerol solutions: mechanism

2009 ◽  
Vol 3 (1-2) ◽  
pp. 44-52 ◽  
Author(s):  
Xiang Wen ◽  
Zongzhen Mao ◽  
Zhenzhen Han ◽  
Valery V. Tuchin ◽  
Dan Zhu
Keyword(s):  
Optical Clearing

scholarly journals Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents

2020 ◽  
Vol 245 (18) ◽  
pp. 1629-1636
Author(s):  
Ruiming Kong ◽  
Wenjuan Wu ◽  
Rui Qiu ◽  
Lei Gao ◽  
Fengxian Du ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Light Attenuation ◽  
Anterior Segment ◽  
Posterior Segment ◽  
Glycerol Solution ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Imaging Depth

Optical coherence tomography has become an indispensable diagnostic tool in ophthalmology for imaging the retina and the anterior segment of the eye. However, the imaging depth of optical coherence tomography is limited by light attenuation in tissues due to optical scattering and absorption. In this study of rabbit eye both ex vivo and in vivo, optical coherence tomography imaging depth of the anterior and posterior segments of the eye was extended by using optical clearing agents to reduce multiple scattering. The sclera, the iris, and the ciliary body were clearly visualized by direct application of glycerol at an incision on the conjunctiva, and the posterior boundary of sclera and even the deeper tissues were detected by submerging the posterior segment of eye in glycerol solution ex vivo or by retro-bulbar injection of glycerol in vivo. The ex vivo rabbit eyes recovered to their original state in 60 s after saline-wash treatment, and normal optical coherence tomography images of the posterior segment of the sample eyes proved the self-recovery of in vivo performance. Signal intensities of optical coherence tomography images obtained before and after glycerol treatment were compared to analysis of the effect of optical clearing. To the best of our knowledge, this is the first study for imaging depth extension of optical coherence tomography in both the anterior and posterior segments of eye by using optical clearing agents.

Integrated effects of fractional laser microablation and sonophoresis on skin immersion optical clearing in vivo

2020 ◽  
Vol 13 (7) ◽  
Author(s):  
Elina A. Genina ◽  
Alexey N. Bashkatov ◽  
Georgy S. Terentyuk ◽  
Valery V. Tuchin
Keyword(s):  
Fractional Laser ◽  
Optical Clearing

The role of optical clearing to enhance the applications of in vivo OCT and photodynamic therapy: Towards PDT of pigmented melanomas and beyond

2021 ◽  
pp. 569-576
Author(s):  
Layla Pires ◽  
Michelle Barreto Requena ◽  
Valentin Demidov ◽  
Ana Gabriela Salvio ◽  
I. Alex Vitkin ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Optical Clearing

Kinetics of optical clearing of human skin studied in vivo using portable Raman spectroscopy

2020 ◽  
Vol 17 (10) ◽  
pp. 105601
Author(s):  
Qingyu Lin ◽  
Ekaterina N Lazareva ◽  
Vyacheslav I Kochubey ◽  
Yixiang Duan ◽  
Valery V Tuchin
Keyword(s):  
Raman Spectroscopy ◽  
Human Skin ◽  
Optical Clearing ◽  
Portable Raman Spectroscopy ◽  
Kinetics Of ◽  
Portable Raman

Dynamics of optical clearing of human skin in vivo

2000 ◽  
Author(s):  
Svetlana P. Chernova ◽  
Natal'ya V. Kuznetsova ◽  
Alexander B. Pravdin ◽  
Valery V. Tuchin
Keyword(s):  
Human Skin ◽  
Optical Clearing
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