l-Arginine ameliorates oxidative stress in alloxan-induced experimental diabetes mellitus

2004 ◽  
Vol 24 (2) ◽  
pp. 93-97 ◽  
Author(s):  
M. A. El-Missiry ◽  
A. I. Othman ◽  
M. A. Amer
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Experimental Diabetes Mellitus

The effect of melatonin on oxidative stress and apoptosis in experimental diabetes mellitus-related ovarian injury

2016 ◽  
Vol 32 (5) ◽  
pp. 421-426 ◽  
Author(s):  
Umit Nayki ◽  
Didem Onk ◽  
Gurhan Balci ◽  
Cenk Nayki ◽  
Alper Onk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Experimental Diabetes Mellitus

scholarly journals The effect of photobiomodulation therapy of some indices of rats’ blood cells functional state under experimental diabetes mellitus

2021 ◽  
Vol 15 (3) ◽  
pp. 3-16
Author(s):  
O. I. Karmash ◽  
◽  
M. Ya. Liuta ◽  
N. V. Yefimenko ◽  
N. O. Sybirna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Catalase Activity ◽  
Experimental Diabetes ◽  
Blood System ◽  
No Synthase ◽  
Photobiomodulation Therapy ◽  
Antioxidant Systems ◽  
Experimental Diabetes Mellitus ◽  
Inducible No Synthase

Background. Diabetes mellitus is a chronic endocrine-metabolic disease caused by an absolute or relative insulin deficiency. During diabetes, there are perfect conditions for the development of oxidative stress: the content of substrates for oxidation increases, the content of natural antioxidants decreases and the activity of antioxidant systems is suppressed. It is known that photobiomodulation therapy produce antioxidant and antihyperglycemic effects. Here we investigated its influence on blood system functioning. Materials and Methods. The study was performed on male Wistar rats. Experimental diabetes mellitus was induced by the intraperitoneal injection of streptozotocin. Leukocyte formula was calculated using blood smears stained by Romanowsky–Giemsa. Catalase activity was determined spectrophotometrically. Affinity of hemoglobin to oxygen was evaluated by spectrophotometric method in Ivanov’s modification by drawing oxygenation curves. The protoporphyrin content in whole blood was measured by analyzing its fluorescence spectra. The content of NO2-, total and inducible NO synthase activity was determined spectrophotometrically. Results. Under the action of photobiomodulation therapy on healthy animals, there was a shift of oxygenation curves to the left and a decrease of P50, whereas under irradiation of rats with diabetes, there was a shift of oxygenation curves to the right and increase in P50 compared to indices in nonirradiated animals. During diabetes, there was a decrease in protoporphyrin content compared to control, but there was a tendency to increase under photobiomodulation. Photobiomodulation therapy of rats with diabetes increased catalase activity in erythrocyte hemolysates. We revealed significant changes in leukocyte formula under photobiomodulation. The total NO synthase activity in leukocytes of rats with diabetes was higher compared to healthy animals, but decreased under the action of photobiomodulation. We found an increase in inducible NO synthase activity in leukocytes of rats with diabetes and in leukocytes of irradiated healthy animals. An increase in NO2- content in leukocytes of rats with diabetes was detected. Under photobiomodulation, NO2- content was significantly lower in rats with diabetes. Conclusion. Photobiomodulation therapy produces a corrective action on blood system during diabetes, in particular, it improves oxygen release from hemoglobin and prevents hypoxia. Simultaneously with the increase in tissue oxygen saturation, a decrease in NO synthase activity and nitrite content along with an increase in catalase activity prevents the development of oxidative stress.

Correction of oxidative stress in experimental diabetes mellitus by means of natural antioxidants

2021 ◽  
Author(s):  
Talat Saatov ◽  
Mukhammadjon Mustafakulov ◽  
Sanobarkhon Irgasheva ◽  
Zafar Ibragimov ◽  
Elvira Ibragimova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Natural Antioxidants ◽  
Experimental Diabetes Mellitus

scholarly journals Peroxisomal Enzymes in the Liver of Rats With Experimental Diabetes Mellitus Type 2

2014 ◽  
pp. S585-S591 ◽  
Author(s):  
L. TURECKÝ ◽  
V. KUPČOVÁ ◽  
E. UHLÍKOVÁ ◽  
V. MOJTO
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Diabetes Mellitus Type 2 ◽  
Experimental Diabetes ◽  
Beta Oxidation ◽  
Experimental Diabetes Mellitus

Diabetes mellitus is relatively frequently associated with fatty liver disease. Increased oxidative stress probably plays an important role in the development of this hepatopathy. One of possible sources of reactive oxygen species in liver is peroxisomal system. There are several reports about changes of peroxisomal enzymes in experimental diabetes, mainly enzymes of fatty acid oxidation. The aim of our study was to investigate the possible changes of activities of liver peroxisomal enzymes, other than enzymes of beta-oxidation, in experimental diabetes mellitus type 2. Biochemical changes in liver of experimental animals suggest the presence of liver steatosis. The changes of serum parameters in experimental group are similar to changes in serum of patients with non-alcoholic fatty liver disease. We have shown that diabetes mellitus influenced peroxisomal enzymes by the different way. Despite of well-known induction of peroxisomal beta-oxidation, the activities of catalase, aminoacid oxidase and NADH-cytochrome b5 reductase were not significantly changed and the activities of glycolate oxidase and NADP-isocitrate dehydrogenase were significantly decreased. The effect of diabetes on liver peroxisomes is probably due to the increased supply of fatty acids to liver in diabetic state and also due to increased oxidative stress. The changes of metabolic activity of peroxisomal compartment may participate on the development of diabetic hepatopathy.

Sign in / Sign up

Export Citation Format

Share Document