Gender difference in hepatic toxicity of titanium dioxide nanoparticles after subchronic oral exposure in Sprague‐Dawley rats

2019 ◽  
Vol 39 (5) ◽  
pp. 807-819 ◽  
Author(s):  
Zhangjian Chen ◽  
Di Zhou ◽  
Shupei Zhou ◽  
Guang Jia
Keyword(s):  
Titanium Dioxide ◽  
Gender Difference ◽  
Titanium Dioxide Nanoparticles ◽  
Oral Exposure ◽  
Hepatic Toxicity ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Effect of oral exposure to titanium dioxide nanoparticles on lipid metabolism in Sprague-Dawley rats

Nanoscale ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 5973-5986 ◽  
Author(s):  
Zhangjian Chen ◽  
Shuo Han ◽  
Pai Zheng ◽  
Di Zhou ◽  
Shupei Zhou ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Lipid Metabolism ◽  
Titanium Dioxide Nanoparticles ◽  
Oral Exposure ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

The present study investigated the effect of oral exposure to TiO2 NPs on lipid metabolism by serum lipidomics.

Serum metabolomic signatures of Sprague-Dawley rats after oral administration of titanium dioxide nanoparticles

NanoImpact ◽  
2020 ◽  
Vol 19 ◽  
pp. 100236
Author(s):  
Zhangjian Chen ◽  
Shuo Han ◽  
Di Zhou ◽  
Pai Zheng ◽  
Shupei Zhou ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Oral Administration ◽  
Titanium Dioxide Nanoparticles ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Assessment of Titanium Dioxide Nanoparticles (TiO2-NPs) Induced Hepatotoxicity and Ameliorative Effects of Cinnamomum cassia in Sprague-Dawley Rats

2017 ◽  
Vol 182 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Muhammad Shakeel ◽  
Farhat Jabeen ◽  
Rehana Iqbal ◽  
Abdul Shakoor Chaudhry ◽  
Sadia Zafar ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Sprague Dawley ◽  
Tio2 Nps ◽  
Cinnamomum Cassia ◽  
Sprague Dawley Rats

scholarly journals Landscape of Lipidomic Metabolites in Gut-liver Axis of Sprague-Dawley Rats after Oral Exposure to Titanium Dioxide Nanoparticles

2020 ◽  
Author(s):  
Zhangjian Chen ◽  
Shuo Han ◽  
Pai Zheng ◽  
Jiahe Zhang ◽  
Shupei Zhou ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Lipid Metabolism ◽  
Liver Toxicity ◽  
Titanium Dioxide Nanoparticles ◽  
Food Additives ◽  
Antibacterial Properties ◽  
Oral Exposure ◽  
Sprague Dawley ◽  
Serum Samples ◽  
Lipid Metabolism Disorder

Abstract Background: The application of titanium dioxide nanoparticles (TiO2 NPs) as food additives poses a risk of oral exposure that may lead to adverse health effects. Even though the substantial evidence supported liver as the target organ of TiO2 NPs via oral exposure, the mechanism of liver toxicity remains largely unknown. Since the liver is a key organ for lipid metabolism, this study focused on the landscape of lipidomic metabolites in gut-liver axis of Sprague Dawley (SD) rats exposed to TiO2 NPs at 0, 2, 10, 50 mg/kg body weight per day for 90 days.Results: TiO2 NPs (50 mg/kg) caused slight hepatotoxicity and changed lipidomic signatures of main organs or systems in the gut-liver axis including liver, serum and gut. The cluster profile from the above biological samples all pointed to the same key metabolic pathway and metabolites, which was glycerophospholipid metabolism and Phosphatidylcholines (PCs), respectively. In addition, absolute quantitative lipidomics verified the changes of three PCs concentrations, including PC(16:0/20:1), PC(18:0/18:0) and PC(18:2/20:2) in the serum samples after treatment of TiO2 NPs (50 mg/kg). The contents of malondialdehyde (MDA) in serum and liver increased significantly, which were positively correlated with most differential lipophilic metabolites.Conclusions: The gut was presumed to be the original site of oxidative stress and disorder of lipid metabolism, which resulted in hepatotoxicity through the gut-liver axis. Lipid peroxidation may be the initial step of lipid metabolism disorder induced by TiO2 NPs. Most nanomaterials (NMs) have oxidation induction and antibacterial properties, so the toxic pathway revealed in the present study may be primary and universal.

Evaluation of Reproductive Development Following Intravenous and Oral Exposure to DEHP in Male Neonatal Rats

2003 ◽  
Vol 22 (3) ◽  
pp. 159-174 ◽  
Author(s):  
Jon N. Cammack ◽  
Randy D. White ◽  
Donovan Gordon ◽  
Jerome Gass ◽  
Lawrence Hecker ◽  
...  
Keyword(s):  
Sperm Count ◽  
Liver Weight ◽  
Oral Exposure ◽  
Seminiferous Tubules ◽  
Primary Group ◽  
Sprague Dawley ◽  
Intravenous Injections ◽  
Sprague Dawley Rats ◽  
Recovery Group ◽  
Ethylhexyl Phthalate

Di-(2-ethylhexyl)phthalate (DEHP) was administered to 3- to 5-day-old male Sprague-Dawley rats by daily intravenous injections of 60, 300, or 600 mg/kg/day or by daily oral gavage of 300 or 600 mg/kg/day for 21 days. Histopathological evaluation and organ weight measurements were performed on some animals after 21 days of dosing (primary group) and later on the recovery group animals that were held without further treatment until sexual maturity at approximately 90 days of age. No effects of any type were observed in animals treated intravenously with 60 mg/kg/day. Testicular changes, consisting of a partial depletion of the germinal epithelium and/or decrease in diameter of seminiferous tubules, were present in all animals of the 300- and 600-mg/kg/day groups after the 21-day dosing period. Testes weight decreased and liver weight increased in these animals. Testes changes were dose-related and generally more severe among animals dosed orally versus intravenously. In the recovery animals, a residual DEHP-induced decrease in seminiferous tubule diameter was present in the testis of several animals dosed orally at 300 and 600 mg/kg/day, but not in animals dosed intravenously. There was no germinal cell depletion or Sertoli cell alteration observed in any dose group at any time. Notably, no effects on sperm count, sperm morphology, or sperm motility were observed at 90 days of age in any of the groups.

scholarly journals Protective effects of dietary fibre against manganese-induced neurobehavioral aberrations in rats

2012 ◽  
Vol 63 (3) ◽  
pp. 263-270 ◽  
Author(s):  
Xiu-Quan Shi ◽  
Wei Yan ◽  
Ke-Yue Wang ◽  
Qi-Yuan Fan ◽  
Yan Zou
Keyword(s):  
Cerebral Cortex ◽  
Animal Feed ◽  
Oral Exposure ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Adult Rats ◽  
Neurobehavioral Performance ◽  
Sprague Dawley Rats ◽  
Applied Dose ◽  
Mn Concentrations

We tested the hypothesis that dietary fi bre (DF) has protective effects against manganese (Mn)-induced neurotoxicity. Forty-eight one-month old Sprague-Dawley rats were randomly divided into six groups: control, 16 % DF, Mn (50 mg kg-1 body weight), Mn+ 4 % DF, Mn+ 8 % DF, and Mn+ 16 % DF. After oral administration of Mn (as MnCl2) by intragastric tube during one month, we determined Mn concentrations in the blood, liver, cerebral cortex, and stool and tested neurobehavioral functions. Administration of Mn was associated with increased Mn concentration in the blood, liver, and cerebral cortex and increased Mn excretion in the stool. Aberrations in neurobehavioral performance included increases in escape latency and number of errors and decrease in step-down latency. Irrespective of the applied dose, the addition of DF in forage decreased tissue Mn concentrations and increased Mn excretion rate in the stool by 20 % to 35 %. All neurobehavioral aberrations were also improved. Our fi ndings show that oral exposure to Mn may cause neurobehavioral abnormalities in adult rats that could be effi ciently alleviated by concomitant supplementation of DF in animal feed.

Exploration of cytotoxic and genotoxic endpoints following sub-chronic oral exposure to titanium dioxide nanoparticles

2019 ◽  
Vol 35 (9) ◽  
pp. 577-592 ◽  
Author(s):  
Srijita Chakrabarti ◽  
Danswrang Goyary ◽  
Sanjeev Karmakar ◽  
Pronobesh Chattopadhyay
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Flow Cytometric Analysis ◽  
Raw 264.7 Cells ◽  
Oral Exposure ◽  
Chromosomal Breakage ◽  
Flow Cytometric ◽  
Higher Doses

Health hazards of titanium dioxide nanoparticles (TiO2-NPs) have raised severe concerns because of the paucity of information regarding the toxic effects among the population. In the present research, the in vitro and in vivo cytotoxic potential of TiO2-NPs were evaluated using flow cytometric techniques. Further, in vitro and in vivo genotoxic endpoints were estimated by means of comet, micronucleus (MN), and chromosomal aberration (CA) assays. In vitro analysis was performed at the concentration range of 10–100 µg/mL using murine RAW 264.7 cells. In vivo experiments were conducted on Albino mice (M/F) by exposing them to 200 and 500 mg/kg TiO2-NPs for 90 days. Decreased percentage of cell viability with higher doses of TiO2-NPs was evident in both in vitro and in vivo flow cytometric analysis. Further, an impaired cell cycle (G0/G1, S, and G2/M) was reflected in the present investigation following the exposure to TiO2-NPs. Increased comet scores such as tail length, % DNA in tail, tail moment, and olive moment were also observed with the higher doses of TiO2-NPs in vitro and in vivo comet assays. Finally, the in vivo MN and CA assays revealed the formation of MN and chromosomal breakage following the exposure to TiO2-NPs.

scholarly journals Dose-dependent acceleration in the delayed effects of neonatal oral exposure to low-dose 17α-ethynylestradiol on reproductive functions in female Sprague-Dawley rats

2015 ◽  
Vol 40 (6) ◽  
pp. 727-738 ◽  
Author(s):  
Mariko Shirota ◽  
Jun Kawashima ◽  
Tomohiro Nakamura ◽  
Junichi Kamiie ◽  
Kinji Shirota ◽  
...  
Keyword(s):  
Low Dose ◽  
Oral Exposure ◽  
Sprague Dawley ◽  
Delayed Effects ◽  
Sprague Dawley Rats ◽  
Dose Dependent
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