Nanosecond pulsed electric field incorporation technique to predict molecular mechanisms of teratogenicity and developmental toxicity of estradiol-17β on medaka embryos

2017 ◽  
Vol 38 (5) ◽  
pp. 714-723 ◽  
Author(s):  
Akemi Yamaguchi ◽  
Hiroshi Ishibashi ◽  
Susumu Kono ◽  
Midori Iida ◽  
Masaya Uchida ◽  
...  
Keyword(s):  
Electric Field ◽  
Molecular Mechanisms ◽  
Developmental Toxicity ◽  
Pulsed Electric Field ◽  
Nanosecond Pulsed Electric Field ◽  
Estradiol 17Β

scholarly journals Upregulation of PDGF Mediates Robust Liver Regeneration after Nanosecond Pulsed Electric Field Ablation by Promoting the HGF/c-Met Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Junjie Qian ◽  
Jianpeng Liu ◽  
Liangjie Hong ◽  
Haohao Lu ◽  
Danjing Guo ◽  
...  
Keyword(s):  
Liver Function ◽  
Electric Field ◽  
Liver Regeneration ◽  
Molecular Mechanisms ◽  
Pulsed Electric Field ◽  
Serum Levels ◽  
Nanosecond Pulsed Electric Field ◽  
The Impact ◽  
Proliferation Of Hepatocytes

Nanosecond pulsed electric field (nsPEF) has emerged as a promising tool for hepatocellular carcinoma ablation recently. However, little is known about how nsPEF affects liver regeneration while being applied to eliminate liver lesions. Besides, the impact of nsPEF ablation on liver function should also be taken into consideration in the process. In this paper, we study the impact of nsPEF ablation on liver function by the measurement of serum levels of AST and ALT as well as liver regeneration and relevant molecular mechanisms in vivo. We found that mouse liver function exhibited a temporary injury without weight loss after ablation. In addition, local hepatic nsPEF ablation promoted significant proliferation of hepatocytes of the whole liver with an increase in HGF level. Moreover, the proliferation of hepatocytes was dramatically inhibited by the inhibitor of c-Met. Of interest, the periablational area is characterized by high level of PDGF and a large amount of activated hepatic stellate cells. Furthermore, neutralizing PDGF was able to significantly inhibit liver regeneration, the increased HGF level, and the accumulation of activated HSCs. Our findings demonstrated that nsPEF not only was a safe ablation approach but also could stimulate the regeneration of the whole liver through the activation of the HGF/c-Met pathway by upregulation of PDGF within the periablational zone.

scholarly journals Excitation of murine cardiac myocytes by nanosecond pulsed electric field

2019 ◽  
Vol 30 (3) ◽  
pp. 392-401 ◽  
Author(s):  
Jan E. Azarov ◽  
Iurii Semenov ◽  
Maura Casciola ◽  
Andrei G. Pakhomov
Keyword(s):  
Electric Field ◽  
Cardiac Myocytes ◽  
Pulsed Electric Field ◽  
Nanosecond Pulsed Electric Field

scholarly journals Safety and Efficacy of Nanosecond Pulsed Electric Field Treatment of Sebaceous Gland Hyperplasia

2020 ◽  
Vol 46 (6) ◽  
pp. 803-809 ◽  
Author(s):  
Girish S. Munavalli ◽  
Brian D. Zelickson ◽  
Mona M. Selim ◽  
Suzanne L. Kilmer ◽  
Thomas E. Rohrer ◽  
...  
Keyword(s):  
Electric Field ◽  
Sebaceous Gland ◽  
Pulsed Electric Field ◽  
Safety And Efficacy ◽  
Nanosecond Pulsed Electric Field ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

Nanosecond Pulsed Electric Field Lab‐on‐Chip Integrated in Super‐Resolution Microscope for Cytoskeleton Imaging

2019 ◽  
Vol 5 (3) ◽  
pp. 1900669 ◽  
Author(s):  
Daniel Havelka ◽  
Djamel Eddine Chafai ◽  
Ondrej Krivosudský ◽  
Anastasiya Klebanovych ◽  
František Vostárek ◽  
...  
Keyword(s):  
Electric Field ◽  
Super Resolution ◽  
Pulsed Electric Field ◽  
Lab On Chip ◽  
Nanosecond Pulsed Electric Field ◽  
On Chip

scholarly journals Concepts and Capabilities of In-House Built Nanosecond Pulsed Electric Field (nsPEF) Generators for Electroporation: State of Art

2020 ◽  
Vol 10 (12) ◽  
pp. 4244
Author(s):  
Paulius Butkus ◽  
Arūnas Murauskas ◽  
Sonata Tolvaišienė ◽  
Vitalij Novickij
Keyword(s):  
Electric Field ◽  
High Frequency ◽  
Pulsed Electric Field ◽  
Cell Permeabilization ◽  
Pulse Generators ◽  
Nanosecond Pulsed Electric Field ◽  
Forming Characteristics ◽  
State Of Art ◽  
Frequency Pulse

Electroporation is a pulsed electric field triggered phenomenon of cell permeabilization, which is extensively used in biomedical and biotechnological context. There is a growing scientific demand for high-voltage and/or high-frequency pulse generators for electropermeabilization of cells (electroporators). In the scope of this article we have reviewed the basic topologies of nanosecond pulsed electric field (nsPEF) generators for electroporation and the parametric capabilities of various in-house built devices, which were introduced in the last two decades. Classification of more than 60 various nsPEF generators was performed and pulse forming characteristics (pulse shape, voltage, duration and repetition frequency) were listed and compared. Lastly, the trends in the development of the electroporation technology were discussed.

scholarly journals Effects of Nanosecond Pulsed Electric Field on Intracellular NADH Autofluorescence: A Comparison between Normal and Cancer Cells

ACS Omega ◽  
2017 ◽  
Vol 2 (6) ◽  
pp. 2916-2924 ◽  
Author(s):  
Kamlesh Awasthi ◽  
Takakazu Nakabayashi ◽  
Liming Li ◽  
Nobuhiro Ohta
Keyword(s):  
Electric Field ◽  
Cancer Cells ◽  
Pulsed Electric Field ◽  
Nanosecond Pulsed Electric Field

Nanosecond pulsed electric field induced changes in cell surface charge density

Micron ◽  
2017 ◽  
Vol 100 ◽  
pp. 45-49 ◽  
Author(s):  
Diganta Dutta ◽  
Xavier-Lewis Palmer ◽  
Anthony Asmar ◽  
Michael Stacey ◽  
Shizhi Qian
Keyword(s):  
Cell Surface ◽  
Electric Field ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Pulsed Electric Field ◽  
Cell Surface Charge ◽  
Nanosecond Pulsed Electric Field ◽  
Induced Changes
Sign in / Sign up

Export Citation Format

Share Document