scholarly journals Relationship between increasing concentrations of two carcinogens and statistical image descriptors offocimorphology in the cell transformation assay

2016 ◽  
Vol 37 (6) ◽  
pp. 709-720
Author(s):  
Giulia Callegaro ◽  
Raffaella Corvi ◽  
Susan Salovaara ◽  
Chiara Urani ◽  
Federico M. Stefanini
Keyword(s):  
Cell Transformation ◽  
Transformation Assay ◽  
Image Descriptors

Objective scoring of transformed foci in BALB/c 3T3 cell transformation assay by statistical image descriptors

2013 ◽  
Vol 27 (6) ◽  
pp. 1905-1912 ◽  
Author(s):  
C. Urani ◽  
R. Corvi ◽  
G. Callegaro ◽  
F.M. Stefanini
Keyword(s):  
Cell Transformation ◽  
Transformation Assay ◽  
Image Descriptors

An Optimised Data Analysis for the Balb/c3T3 Cell Transformation Assay and its Application to Metal Compounds

2007 ◽  
Vol 20 (4) ◽  
pp. 673-684 ◽  
Author(s):  
J. Ponti ◽  
B. Munaro ◽  
M. Fischbach ◽  
S. Hoffmann ◽  
E. Sabbioni
Keyword(s):  
Data Analysis ◽  
Cell Transformation ◽  
T Test ◽  
Alternative Methods ◽  
Metal Compounds ◽  
Exact Test ◽  
Fisher's Exact Test ◽  
Transformation Assay ◽  
Carcinogenic Potential ◽  
Fisher’S Exact Test

The Balb/c3T3 cell transformation assay (CTA) is an available in vitro system to detect the carcinogenic potential of chemicals. Currently, the European Centre for the Validation of Alternative Methods (ECVAM) is validating this test, assessing its reliability and relevance. Its endpoint is the formation of type III foci, which is, when using clone A31-1-1, a very rare event that usually does not occur at all for negative controls. The carcinogenic potential of a compound tested is assessed by comparing the number of foci in treated and untreated cells. The objective of the present work is to optimise the data analysis for this endpoint by applying the most commonly used approach by a t-test and the Fisher's exact test as an alternative approach. For this purpose selected metal compounds classified as carcinogenic (NaAsO2, CdCl2 cisPt), as suspected carcinogenic (C6H5)4AsCl, CH3HgCl), or as compounds without evidence of carcinogenic properties in humans ((NH4)2PtCl6, NaVO3) as well as a non-carcinogenic (AgNO3) were analysed. Our evaluation revealed that the t-test approach, which assumes normality of data, is not appropriate. The results demonstrated that the statistical analysis by Fisher's exact test better reflects the data properties and greatly facilitates the interpretation of Balb/c3T3 CTA data regarding carcinogenic potential.

Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens

2014 ◽  
pp. 343-362 ◽  
Author(s):  
Kiyoshi Sasaki ◽  
Anna Huk ◽  
Naouale El Yamani ◽  
Noriho Tanaka ◽  
Maria Dusinska
Keyword(s):  
Cell Transformation ◽  
Transformation Assay ◽  
Genotoxic Carcinogens

SHE Cell Transformation Assay as a Predictive System for Carcinogenicity of PAH and Non-Genotoxic Carcinogens

1996 ◽  
Vol 10 (1-4) ◽  
pp. 307-314 ◽  
Author(s):  
Veronique Cruciani ◽  
Halima Bessi ◽  
Stephanie Alexandre ◽  
Marie-Jose Durand ◽  
Claudine Rast ◽  
...  
Keyword(s):  
Cell Transformation ◽  
Transformation Assay ◽  
Genotoxic Carcinogens

An interlaboratory evaluation of the Syrian hamster embryo cell transformation assay using eighteen coded chemicals

1988 ◽  
Vol 2 (2) ◽  
pp. 103-116 ◽  
Author(s):  
C.A. Jones ◽  
E. Huberman ◽  
M.F. Callaham ◽  
A. Tu ◽  
W. Halloween ◽  
...  
Keyword(s):  
Syrian Hamster ◽  
Cell Transformation ◽  
Embryo Cell ◽  
Transformation Assay ◽  
Hamster Embryo Cell
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